Abstract
Aloe vera is a traditional medicinal plant; however, its use in fish is fairly recent. We evaluated the effects of dietary A. vera on stress, innate immunity, and energy metabolism in pacu inoculated with Aeromonas hydrophila. For 7 days, 192 fish were fed with diets supplemented with 0% (control), 0.5%, 1.0%, and 2.0% of the plant extract and then inoculated with bacteria and sampled 3, 6, and 24 h later. All concentrations of A. vera reduced basal levels of cortisol, and 1.0% reduced cortisol levels more intensely 3 h after inoculation. A. vera increased the basal respiratory activity of leukocytes/RAL (0.5 and 1.0%), increased the serum levels of lysozyme (1.0 and 2.0%) 6 h after inoculation, and increased the activity of the complement system after 3 h. Spleen somatic index/SSI increased with 1.0 and 2.0% A. vera. A. vera also promoted metabolic effects. It increased basal levels of lipids in the liver and muscle, as well as hepatosomatic index (1.0%) and, 3 h after inoculation, prevented the reduction of serum triglyceride (1.0%) and reduced the mesenteric fat (1.0%). Bacterial inoculation increased RAL from 3 to 24 h and lysozyme levels at 24 h, increased serum cholesterol at 24 h, and decreased serum triglyceride from 3 to 24 h, regardless of A. vera. We concluded that A. vera offered for only 7 days had stress-reducing effects, stimulated innate immunity, protected triglyceride levels in blood, lipid depots in the liver and muscle, and directed the energy mobilization to visceral depots.
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References
Agius C, Roberts RJ (2003) Melano-macrophage centres and their role in fish pathology. J Fish Dis 26:499–509
Ahlawat KS, Khatkar BS (2011) Processing, food applications and safety of aloe vera products: a review. J Food Sci Technol 48:525–533
Alinejad-Mofrad S, Foadoddini M, Saadatjoo SA, Shayesteh M (2015) Improvement of glucose and lipid profile status with Aloe vera in pre-diabetic subjects: a randomized controlled-trial. J Diabetes Metab Disord 14:22
Alishahi M, Ranjbar MM, Ghorbanpour M, Peyghan R, Mesbah M, Razijalali M (2010) Effects of dietary Aloe vera on some specific and nonspecific immunity in the common carp (Cyprinus carpio). Int J Vet Res 4:189–195
Alishahi M, Dezfuly ZT, Mohammadian T, Mesbah M (2017) Effects of Aloe Vera crude extract on growth performance and some hemato-immunological indices of Oncorhynchus mykiss in farm scale. Iran J Vet Med 10:383–394
Anderson DP, Siwicki AK (1995) Basic haematology and serology for fish health programs, diseases in Asian aquaculture II, Manila: Fish Health Section, Asian Fisheries Society 185–202
Awad E, Awaad (2017) A role of medicinal plants on growth performance and immune status in fish. Fish Shellfish Immunol 67:40–54
Bairwa MK, Jakhar J, Reddy AD (2012) Animal and plant originated immunostimulants used in aquaculture. J Nat Prod Plant Resour 2:397–400
Biller-Takahashi JD, Takahashi LS, Saita MV, Gimbo RY, Urbinati EC (2013) Leucocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus. Braz J Biol 73:425–429
Boshra H, Li J, Sunyer JO (2006) Recent advances on the complement system of teleost fish. Fish Shellfish Immunol 20:239–262
Bulfon C, Volpatti D, Galeotti M (2015) Current research on the use of plant-derived products in farmed fish. Aquac Res 46:513–551
Choudhary M, Kochhar A, Sangha J (2014) Hypoglycemic and hypolipidemic effect of Aloe vera L. in non-insulin dependent diabetics. J Food Sci Technol 51:90–96
Citarasu G (2010) Herbal biomedicines: a new opportunity for aquaculture industry. Aquac Int 18:403–414
Demers NE, Bayne CJ (1997) The immediate effects of stress on hormones and plasma lysozyme in rainbow trout. Dev Comp Immunol 21:363–373
Dotta G, Andrade JIA, Gonçalves ILT, Brum A, Mattos JJ, Maraschin M, Martins ML (2014) Leukocyte phagocytosis and lysozyme activity in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis. Fish Shellfish Immunol 39:280–284
FAO (2018). The State of World Fisheries and Aquaculture. Available at: http://www.fao.org/3/I9540EN/i9540en.pdf
Farahi A, Kasiri M, Sudagar M, Soleimani IM, Zorriehzahra SMJ (2012) Effect of dietary supplementation of Melisa officialis and Aloe vera on haematological traits, lipid oxidation of carcass and performance in rainbow trout (Oncorhynchus mykiss). Online J Anim Feed Res 1:1–5
Ferreira MW, Santos RM, Silva AR, Marino ED, Makimoto SS, Barbosa GRC, Andrade GB (2019) Mortality in pacus (Piaractus mesopotamicus) caused by Pantoea agglomerans and Pseudomonas aeruginosa in excavated tank. Acta Sci Vet 47:379
Ferriani VPL, Barbosa JE, Carvalho IF (1990) Serum hemolytic classical and alternative pathways of complement in infancy - age-related-changes. Acta Paediatr Scand 79:322–327
Folch J, Less M, Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–503
Frings CS, Fendly TW, Dunn RT, Quenn CA (1972) Improved determination of total lipids by the sulpho-phospho-vanilin reaction. Clin Chem 18:673–674
Gabriel NN, Qiang J, Ma XY, He J, Xu P, Liu K (2015a) Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge. Fish Physiol Biochem 41:1321–1332
Gabriel NN, Qiang J, He J, Ma XY, Kpundeh MD, Xu P (2015b) Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Fish Shellfish Immunol 44:504–514
Gabriel NN, Wilhelm MR, Habte-Tsion H-M, Chimwamurombe P, Omoregie E, Iipinge LN, Shimooshili K (2019) Effect of dietary Aloe vera polysaccharides supplementation on growth performance, feed utilization, hemato-biochemical parameters, and survival at low pH in African catfish (Clarias gariepinus) fingerlings. Int Aquat Res 11:57–72
Galina J, Yin G, Ardó L, Jeney Z (2009) The use of immunostimulating herbs in fish. An overview of research. Fish Physiol Biochem 35:669–676
Garcia-Abiado MA, Mbahinzireki G, Rinchard J, Lee KJ, Dabrowski K (2004) Effect of diets containing gossypol on blood parameters and spleen structure in tilapia, Oreochromis sp., reared in a recirculating system. J Fish Dis 27:359–368
Gogal RM, Smith BJ, Robertson JL, Smith SA, Holladay SD (1999) Tilapia (Oreochromis niloticus) dosed with azathrioprine display immune effects similar to those seen in mammals, including apoptosis. Vet Immunol Immunopathol 68:209–227
Haghighi M, Rohani MS, Samadi M, Tavoli M, Eslami M, Yusefi R (2014) Study of effects Aloe vera extract supplemented feed on hematological and immunological indices of rainbow trout (Oncorhynchus mykiss). Int J Adv Biol Biomed Res 2:2143–2154
Hai NV (2015) The use of medicinal plants as immunostimulants in aquaculture: a review. Aquaculture 446:88–96
Hamman JH (2008) Composition and applications of Aloe vera leaf gel. Molecules 13:1599–1616
Harikrishnan R, Balasundaram C, Heo M-S (2011) Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture 317:1–15
Havixbeck JJ, Barreda DR (2015) Neutrophil development, migration, and function in teleost fish. Biology 4:715–734
Im SA, Oh ST, Song S, Kim MR, Kim DS, Woo SS, Jo TH, Park YI, Lee CK (2005) Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. Int Immunopharmacol 5:271–279
Kim KH, Hwang YJ, Bai SC (1999) Resistance to Vibrio alginolyticus in juvenile rockfish Sebastes schlegeli / fed diets containing different doses of aloe. Aquaculture 180:13–21
Lieke T, Meinelt T, Hoseinifar SH, Pan B, Straus DL, Steinberg CEW (2019) Sustainable aquaculture requires environmental-friendly treatment strategies for fish diseases. Rev Aquac:1–23
Luta G, McAnalley BH (2005) Aloe vera: chemical composition and methods used to determine its presence in commercial products. GlycoSci Nutr 6(4):1–12
Mahdavi M, Hajimoradloo A, Ghorbani R (2013) Effect of Aloe vera extract on growth parameters of common carp (Cyprinus carpio). World J Medical Sci 9:55–60
Mehrabi Z, Firouzbakhsh F, Rahimi-Mianji G, Paknejad H (2019) Immunostimulatory effect of Aloe vera (Aloe barbadensis) on non-specific immune response, immune gene expression, and experimental challenge with Saprolegnia parasitica in rainbow trout (Oncorhynchus mykiss). Aquaculture 503:330–338
Miranda CD, Godoy FA, Lee MR (2018) Current status of the use of antibiotics and the antimicrobial resistance in the Chilean salmon farms. Front Microbiol 9:1284
Moghaddam SB, Haghighi M, Rohani MS, Hamidi M, Ghasemi M (2017) The effects of different levels of Aloe vera extract on some of the hematological and non-specific immune parameters in Siberian sturgeon (Acipenser baerii). Iran J Fish Sci 16:1234–1247
Nguyen GT, Green ER, Mecsas J (2017) Neutrophils to the ROScue: mechanisms of NADPH oxidase activation and bacterial resistance. Front Cell Infect Microbiol 7:373
Palermo FA, Cocci P, Angeletti M, Felici A, Polzonetti-Magni AM, Mosconi G (2013) Dietary Aloe vera components’ effects on cholesterol lowering and estrogenic responses in juvenile goldfish, Carassius auratus. Fish Physiol Biochem 39:851–861
Parrish CC (1999) Determination of total lipid, lipid classes, and fatty acids in aquatic samples, in lipids in freshwater ecosystems. Springer, New York, pp.4–20
Perry SF, Walsh PJ, Mommsen TP, Moon TW (1988) Metabolic consequences of hypercapnia in the rainbow trout Salmo gairdneri: β-adrenergic effects. Gen Comp Endocrinol 69:439–447
Polhill RB, Pruitt KM, Johnston RB (1978) Kinetic assessment of alternative complement pathway activity in a hemolytic system, 1. Experimental and mathematical analyses. J Immunol 121:363–370
Pothuraju R, Sharma RK, Onteru SK, Shaik SS, Hussain A (2016) Hypoglycemic and hypolipidemic effects of Aloe vera extract preparations: a review. Phytother Res 30:200–207
Rajasekaran R, Ravi K, Sivagnanam K, Subramanian S (2006) Beneficial effects of aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clin Exp Pharmacol Physiol 33:232–237
Rajeswari R, Umadevi M, Rahale CS, Pushpa R, Selvavenkadesh S, Kumar KPS, Bhowmik D (2014) Aloe vera: the miracle plant its medicinal and traditional uses in India. J Pharmacogn Phytochem 1:118–124
Reverter M, Bontemps N, Lecchini D, Banaigs B, Sasal P (2014) Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture 433:50–61
Rohani MS, Haghighi M, Moghaddam SB (2017) Study on nanoparticles of Aloe vera extract on growth performance, survival rate and body composition in Siberian sturgeon (Acipenser baerii). Iran J Fish Sci 16:457–468
Sánchez-Machado DI, López-Cervantes J, Sendón R, Sanches-Silva A (2017) Aloe vera: ancient knowledge with new frontiers. Trends Food Sci Technol 61:94–102
Saurabh S, Sahoo PK (2008) Lysozyme: an important defence molecule of fish innate immune system. Aquac Res 39:223–239
Sebastião FA, Furlan LR, Hashimoto DT, Pilarski F (2015) Identification of bacterial fish pathogens in Brazil by direct colony PCR and 16S rRNA gene sequencing. Adv Microbiol 5:409–424
Sheridan MA (1994) Regulation of lipid metabolism in poikilothermic vertebrates. J Comp Biochem Physiol B 107:495–508
Valladão GMR, Gallani SU, Pilarski F (2018) South American fish for continental aquaculture. Rev Aquac 10:351–369
Vaseeharan B, Thaya R (2014) Medicinal plant derivatives as immunostimulants: an alternative to chemotherapeutics and antibiotics in aquaculture. Aquac Int 22:1079–1091
Wang W, Sun J, Liu C, Xue Z (2017) Application of immunostimulants in aquaculture: current knowledge and future perspectives. Aquac Res 48:1–23
WHO, World Health Organization (1999) WHO monographs on selected medicinal plants, vol. 1. Geneva: WHO Publications
WHO World Health Organization (2016) Global principles for the containment of antimicrobial resistance in animals intended for food: report of a WHO consultation with the participation of the food and agriculture Organization of the United Nations and the office international des epizooties. Geneva: World Health Organization; Available at: http://www.who.int/foodsafety/publications/containment-amr/en/ (Accessed 13 Sep 2019)
Yada T, Tort L (2016) Stress and disease resistance: immune system and immunoendocrine interactions. Fish Physiol - Biol Stress Fish 35:365–403
Zanuzzo FS, Biller-Takahashi JD, Urbinati EC (2012) Aloe vera improves the respiratory activity of leukocytes of matrinxã (Brycon amazonicus) during the transport stress. Rev Bras Zootec 41:2299–2302
Zanuzzo FS, Urbinati EC, Rise ML, Hall JR, Nash GW, Gamperl AK (2015a) Aeromonas salmonicida induced immune gene expression in Aloe vera fed steelhead trout Oncorhynchus mykiss (Walbaum). Aquaculture 435:1–9
Zanuzzo FS, Zaiden SF, Senhorini JA, Marzocchi-Machado CM, Urbinati EC (2015b) Aloe vera bath improved physical and humoral protection in breeding stock of matrinxã (Brycon amazonicus) after induced spawning. Fish Shellfish Immunol 45:132–140
Zanuzzo FS, Sabioni RE, Montoya LNF, Favero G, Urbinati EC (2017) Aloe vera enhances the innate immune response of pacu (Piaractus mesopotamicus) after transport stress and combined heat killed Aeromonas hydrophila infection. Fish Shellfish Immunol 65:198–205
Acknowledgments
We thank the Centro de Aquicultura da UNESP (CAUNESP) for supplying the fish for the research and the Laboratório de Microbiologia e Parasitologia de Organismos Aquáticos for supplying the bacteria inoculum.
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The present research has been granted by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [process number: 142171/2016-6].
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All authors contributed to the design of the experiment. The experimental series were conducted by R.W.S.A and supervised by E.C.U. Assays and data analysis were performed by R.W.S.A. with the help from E.C.U. The manuscript was written by both authors.
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de Assis, R.W.S., Urbinati, E.C. Physiological activity of Aloe vera in pacu (Piaractus mesopotamicus) inoculated with Aeromonas hydrophila. Fish Physiol Biochem 46, 1421–1430 (2020). https://doi.org/10.1007/s10695-020-00800-0
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DOI: https://doi.org/10.1007/s10695-020-00800-0