Skip to main content
SpringerLink
Log in

Effects of ribwort plantain (Plantago lanceolata) extract on blood parameters, immune response, antioxidant enzyme activities, and growth performance in rainbow trout (Oncorhynchus mykiss)

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

In this study, we examined changes occurred in blood parameters, immune responses, antioxidant enzyme activities, and growth performance of rainbow trout (Oncorhynchus mykiss) administered with ribwort plantain (RP) through feed. Fish (mean weight 36.56 ± 1.99 g) were fed a diet supplemented with an aqueous methanolic extract of RP at variable doses, 0 (control), 1 (RP1), 2 (RP2), and 3 g kg−1 (RP3) for 90 days. The final weight, weight gain, and specific growth rate were significantly increased in RP1, RP2, and RP3 treatment groups compared to that of the control. Among examined blood parameters, hemoglobin value in RP1 group (9.77 ± 0.10 g dl−1) only was significantly high on the 30th day of the study. When immune response parameters were evaluated, we observed that oxidative radical production and lysozyme activities were affected positively in experimental groups (P < 0.05). The highest oxidative radical production was determined in fish of RP3 group. Glutathione peroxidase and glucose 6 phosphate dehydrogenase were increased in RP3 group compared to control and other treatment groups. Based on these results, it is concluded that ribwort plantain promotes growth, enhances immune responses and antioxidant enzyme activities in rainbow trout, and therefore, may be used in aquaculture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Acar Ü (2018) Sarı kantaron (Hypericum perforatum) yağının sazan yavrularının (Cyprinus carpio) büyüme performansı ve bazı kan parametreleri üzerine etkisi Alınteri Zirai Bilimler Dergisi 33:21–27

  • Almabrok AA, Amhamed ID, Mohamed GA, Bilen S, Altief TAS (2018) Effect of Tilia tomentosa methanolic extract on growth performance, digestive enzyme activity, immune system and haematological indices of common carp (Cyprinus carpio). Mar Sci Technol Bull 7:12–20

    Article  Google Scholar 

  • Altunoglu YC, Bilen S, Ulu F, Biswas G (2017) Immune responses to methanolic extract of black cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 67:103–109

    Article  Google Scholar 

  • Amhamed ID, Mohamed GA, Almabrok AA, Altıef S, Abdalsalam T, Bilen S (2018) Efficacy of dietary Chenopodium album extract on some health parameters, digestive enzymes and growth performance in juvenile Cyprinus carpio Alinteri Journal of Agriculture Sciences 33

  • Aydın, A., Bashimov, G., and Yaykaşlı, M. (2018). The Structural and Economic Analysis of the Rainbow Trout Farming: Case of Erzurum Province. Marine Science and Technology Bulletin, 7(1): 4-11.

  • Baytop T (1999) Therapy with medicinal plants in Turkey (past and present) Publication of the Istanbul University 312

  • Bilen S, Altief TAS, Özdemir KY, Salem MOA, Terzi E, Güney K (2019a) Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem:1–11

  • Bilen S, Altunoglu YC, Ulu F, Biswas G (2016) Innate immune and growth promoting responses to caper (Capparis spinosa) extract in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 57:206–212. https://doi.org/10.1016/j.fsi.2016.08.040

    Article  CAS  PubMed  Google Scholar 

  • Bilen S, Bilen A, Önal U (2015) The effects of oxygen supplementation on growth and survival of rainbow trout (Oncorhynchus mykiss) in different stocking densities. Iran J Fish Sci 14:538–545

    Google Scholar 

  • Bilen S, Bulut M (2010) Effects of Laurel (Laurus nobilis) on the non-specific immune responses of rainbow trout (Oncorhynchus mykiss, Walbaum). J Anim Vet Adv 9:1275–1279

    Article  CAS  Google Scholar 

  • Bilen S, Filogh AM, Ali AB, Kenanoğlu ON, Zoral MA (2019b) Effect of common mallow (Malva sylvestris) dietary supplementation on growth performance, digestive enzyme activities, haemotological and immune responses of common carp (Cyprinus carpio). Aquac Int:1–12

  • Bilen S, Kızak V, Bilen AM (2013a) Floating fish farm unit (3FU). Is it an appropriate method for salmonid production? Mar Sci Technol Bull 2:9–13

    Google Scholar 

  • Bilen S, Özkan O, Alagöz K, Özdemir KY (2018) Effect of dill (Anethum graveolens) and garden cress (Lepidium sativum) dietary supplementation on growth performance, digestive enzyme activities and immune responses of juvenile common carp (Cyprinus carpio). Aquaculture 495:611–616. https://doi.org/10.1016/j.aquaculture.2018.06.037

    Article  CAS  Google Scholar 

  • Bilen S, Soydaş E, Bilen A (2014) Isırgan otunun (Urtica dioica) metanolik özütünün Japon balıklarının (Carassius auratus) doğal olmayan bağışıklık yanıtı üzerine etkileri Alınteri Zirai Bilimler Dergisi 27:24–29

  • Bilen S, Yılmaz S, Bilen AM (2013b) Influence of tetra (Cotinus coggygria) extract against Vibrio anguillarum infection in koi carp, Cyprinus carpio with reference to haematological and immunological changes. Turk J Fish Aquat Sci 13:517–522

    Article  Google Scholar 

  • Blaxhall P, Daisley K (1973) Routine haematological methods for use with fish blood. J Fish Biol 5:771–781

    Article  Google Scholar 

  • Blazer VS (1992) Nutrition and disease resistance in fish. Annu Rev Fish Dis 2:309–323

    Article  Google Scholar 

  • Britton, C. J. (1963). Disorders of the blood. (Text book) 9th ed. J. & A. Churchill LTD, London

  • Cheng, A. C., Tu, C. W., Chen, Y. Y., Nan, F. H., ve Chen, J. C. (2007). The Immunostimulatory Effects of Sodium Alginate and Iota-Carrageenan on Orange- Spotted Grouper Epinephelus coicoides and Its Resistance Against Vibrio alginolyticus. Fish & Shellfish Immunology, 22: 197-205.

  • Christybapita, D., Divyagnaneswari, M., ve Michael, R. D. (2007). Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus. Fish & shellfish immunology, 23(4): 840-852.

  • Diler Ö, Görmez Ö, Terzioğlu S, Atabay A (2015) Pelin Otu (Artemisia vulgaris L)’Nun Gökkuşaği Alabaliklarinda (Oncorhynchus mykiss, Walbaum) Hastaliklara Karşi Direnç Ve Spesifik Olmayan Bağişiklik Sistemi Üzerine Etkisi. J Aquac Eng Fish Res 4:1–11

    Google Scholar 

  • Drabkin DL, Austin JH (1932) Spectrophotometric studies I. Spectrophotometric constants for common hemoglobin derivatives in human, dog, and rabbit blood. J Biol Chem 98:719–733

    CAS  Google Scholar 

  • Dügenci SK, Arda N, Candan A (2003) Some medicinal plants as immunostimulant for fish. J Ethnopharmacol 88:99–106

    Article  Google Scholar 

  • Ellis AE (1990) Lysozyme assays. In: "Techniques in Fish Immunology, Vol. 1" (J.S. Stolen, T.C. Fletcher, D. P. Anderson, B. S. Roberson & W.B. Van Muiswinkel, Eds.), SOS Publications, Fair Haven (USA), 1990.

  • FAO. (2019). Fishery and Aquaculture Statistics 2016. http://www.fao.org/3/i9942t/I9942T.pdf

  • Fazio F (2019) Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture 500:237–242

    Article  Google Scholar 

  • Ferrazzano GF et al. (2015) Determination of the in vitro and in vivo antimicrobial activity on salivary Streptococci and Lactobacilli and chemical characterisation of the phenolic content of a Plantago lanceolata infusion BioMed research international 2015

  • Gabriel NN, Qiang J, Ma XY, He J, Xu P, Liu K (2015) Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge. Fish Physiol Biochem 41:1321–1332

    Article  CAS  Google Scholar 

  • Gökhan HB (2007) BALIKLARDA SERBEST RADİKALLER VE ANTİOKSİDAN SAVUNMA SİSTEMİ

  • Gupta S, Mishra P (2014) Effect of leaf extract of Eclipta alba on hematology of Clarias gariepinus (Burchell, 1822)

  • Hardie L, Ellis A, Secombes C (1996) In vitro activation of rainbow trout macrophages stimulates inhibition of Renibacterium salmoninarum growth concomitant with augmented generation of respiratory burst products. Dis Aquat Organ 25:175–183

    Article  Google Scholar 

  • Hausmann M et al (2007) In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis. Clin Exp Immunol 148:373–381

    Article  CAS  Google Scholar 

  • Kurt B, Bilge N, Sözmen M, Aydın U, Önyay T, Özaydın I (2018) Effects of Plantago lanceolata L. extract on full-thickness excisional wound healing in a mouse model. Biotech Histochem 93:249–257

    Article  CAS  Google Scholar 

  • Laith A et al (2017) Effect of Excoecaria agallocha on non-specific immune responses and disease resistance of Oreochromis niloticus against Streptococcus agalactiae. Res Vet Sci 112:192–200

    Article  CAS  Google Scholar 

  • Lau D., Mollnau H., Eiserich J. P., Freeman B. A., Daiber A., Gehling U. M., Brummer J., Rudolph V., Munzel T., Heitzer T., Meinertz T., Baldus S. (2005) Myeloperoxidase mediates neutrophil activation by association with CD11b/CD18 integrins. Proceedings of the National Academy of Sciences 102 (2):431-436

  • Lewis SM, Bain BJ, Bates I, Dacie JV (2006) Dacie and Lewis practical haematology. Churchill livingstone

  • Manal I (2016) Detoxification and antioxidant effects of garlic and curcumin in Oreochromis niloticus injected with aflatoxin B 1 with reference to gene expression of glutathione peroxidase (GPx) by RT-PCR. Fish Physiol Biochem 42:617–629

    Article  Google Scholar 

  • Metwally M (2009) Effects of garlic (Allium sativum) on some antioxidant activities in tilapia nilotica (Oreochromis niloticus). World J Fish Mar Sci 1:56–64

    CAS  Google Scholar 

  • Mohamed GA, Amhamed ID, Almabrok AA, Barka ABA, Bilen S, Elbishti RT (2018) Effect of celery (Apium graveolens) extract on the growth, haematology, immune response and digestive enzyme activity of common carp (Cyprinus carpio). Mar Sci Technol Bull 7:51–59

    Article  Google Scholar 

  • Murray, A. L., Ponald, J. P., Alcorn, S. W., Fairgrieve, W. T., Shearer, K. D., ve Roley, D. (2003). Ejects Of Various Feed Supplements Containing Fish Protein Hydrolysate Or Fish Processing By Products On The Innate Immune Functions Of Juvenile Coho Salmon (Oncorhynchus kisutch). Aquaculture, 220: 643-653.

  • Nizioł-Łukaszewska Z, Gaweł-Bęben K, Rybczyńska-Tkaczyk K, Jakubczyk A, Karaś M, Bujak T (2019) Biochemical properties, UV-protecting and fibroblast growth-stimulating activity of Plantago lanceolata L. extracts. Ind Crop Prod 138:111453

    Article  Google Scholar 

  • Nobahar Z, Gholipour-Kanani H, Kakoolaki S, Jafaryan H (2015) Effects of garlic (Allium sativum) and nettle (Urtic adioica) on growth performance and on hematological parameters of beluga (Huso huso) نشریه ایرانی سلامت حیوانات آبزی 1:63-69

  • Özkan OE, Güney K, Gür M, Pattabanoğlu ES, Babat E, Khalifa MM (2017) Essential oil of oregano and savory: chemical composition and antimicrobial activity. Indian J Pharm Educ Res 51:S205–S208

    Article  Google Scholar 

  • Ricker, W.E., 1979, Growth rates and models, in: Hoar, W.S., Randall, D.J. & Brett, J.R. (Eds.), Fish Physiology. Academic Press, New York, USA, pp 677–743.

  • Sahoo P, Kumari J, Mishra B (2005) Non-specific immune responses in juveniles of Indian major carps. J Appl Ichthyol 21:151–155

    Article  Google Scholar 

  • Sato Y et al (2011) In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm 403:136–138

    Article  CAS  Google Scholar 

  • Siwicki AK, Anderson DP, Rumsey GL (1994) Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet Immunol Immunopathol 41:125–139

    Article  CAS  Google Scholar 

  • Sönmez AY, Bilen S, Alak G, Hisar O, Yanık T, Biswas G (2015) Growth performance and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss) juveniles fed diets supplemented with sage, mint and thyme oils. Fish Physiol Biochem 41:165–175

    Article  Google Scholar 

  • Srivastava P, Pandey A (2015) Role of immunostimulants in immune responses of fish and shellfish. Biochem Cell Arch 15:47–73

    Google Scholar 

  • Şahan A, Özütok S, Kurutaş EB (2016) Determination of some hematological parameters and antioxidant capacity in Nile tilapia (Oreochromis niloticus Linnaeus, 1758) fed ginger (Zingiber officinale Roscoe) to Aeromonas hydrophila. Turk J Fish Aquat Sci 16:197–204

    Article  Google Scholar 

  • Tafi AA, Meshkini S, Tukmechi A, Alishahi M, Noori F (2018) Immunological and antistreptococcal effects of Salvia officinalis and Aloe vera extracts supplemented feed in rainbow trout (Oncorhynchus mykiss) Kafkas Üniversitesi Veteriner Fakültesi Dergisi 24

  • Temür C, Uslu S (2019) Effects of plantain (Plantago lanceolata) containing diets of quails on growth performance, carcass characteristic, some blood parameters and mast cell numbers Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 29:114–120

Download references

Author information

Authors and Affiliations

  1. Department of Aquaculture, Kastamonu University, Institute of Science, Kastamonu, Turkey

    Randa Taher A. Elbesthi

  2. Faculty of Fisheries, Department of Basic Science, Kastamonu University, Kuzeykent, 37100, Kastamonu, Turkey

    Keriman Yürüten Özdemir & Adem Yavuz Sönmez

  3. Faculty of Fisheries, Department of Aquaculture, Kastamonu University, Kastamonu, Turkey

    Yiğit Taştan & Soner Bilen

Authors
  1. Randa Taher A. Elbesthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Keriman Yürüten Özdemir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yiğit Taştan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Soner Bilen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Adem Yavuz Sönmez
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adem Yavuz Sönmez.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Elbesthi, R.T.A., Özdemir, K.Y., Taştan, Y. et al. Effects of ribwort plantain (Plantago lanceolata) extract on blood parameters, immune response, antioxidant enzyme activities, and growth performance in rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 46, 1295–1307 (2020). https://doi.org/10.1007/s10695-020-00790-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-020-00790-z

Keywords

Search

Navigation