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Effects of dietary exogenous xylanase supplementation on growth performance, intestinal health, and carbohydrate metabolism of juvenile large yellow croaker, Larimichthys crocea

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Abstract

An 8-week feeding trial was conducted to evaluate the effects of dietary xylanase supplementation on growth performance, digestive enzyme activity, intestinal morphology parameter, intestinal microbiome diversity, and carbohydrate metabolism for juvenile large yellow croaker (Larimichthys crocea). Four levels of xylanase were added to basal diets (0, 600, 1200, and 1800 U kg−1). The results indicated that fish fed the 1200 U kg−1 xylanase diet had higher weight gain than those fed the 0 and 600 U kg−1 xylanase diet. The highest intestinal folds and microvillous height were observed at fish fed the 1200 U kg−1 xylanase diet. High-throughput sequencing revealed that the majority of reads derived from the large yellow croaker digesta belonged to members of Proteobacteria followed by Chloroflex, Bacteroidetes, Spirochaetae, and Firmicute. Supplementation of xylanase in diets increased the relative abundance of Bacteroides and Gemmatimonadete. The higher hepatic glucokinase (GK) and glucose-6-phosphate dehydrogenase (G6PD) activities were observed in fish fed the xylanase supplementation diet. Accordingly, dietary xylanase supplementation upgraded the relative expressions of gk and g6pd genes in liver. In conclusion, optimum dietary xylanase supplementation (600-1200 U kg−1) could improve the growth performance, optimize the intestinal morphology structure and microbiota constitution, and enhance the ability of carbohydrate utilization of juvenile large yellow croaker.

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Acknowledgments

We would like to thank Qiu, H., Sun, P., and Yuan, Y. for their valuable help with this study.

Funding

This study was supported by China Agriculture Research System-48 (CARS-48), Key Research and Development Plan Project of Zhejiang Province (2018C02037), the Nature Science Foundation of Zhejiang Province (LY17C190002), and the Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University, and by the K. C. Wong Magna Fund and K. C. Wong Education Foundation at Ningbo University.

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  1. Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo, 315211, China

    Jiaxiang Luo, Yi Li, Min Jin, Tingting Zhu, Chenchen Li & Qicun Zhou

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  1. Jiaxiang Luo
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  2. Yi Li
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Luo, J., Li, Y., Jin, M. et al. Effects of dietary exogenous xylanase supplementation on growth performance, intestinal health, and carbohydrate metabolism of juvenile large yellow croaker, Larimichthys crocea. Fish Physiol Biochem 46, 1093–1110 (2020). https://doi.org/10.1007/s10695-020-00774-z

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