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Efficacy of dietary exogenous enzyme supplementation on growth performance, antioxidant activity, and digestive enzymes of common carp (Cyprinus carpio) fry

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Abstract

Fishmeal (FM) is the main source of protein in fish diets, but its supply is stable. So, available plant protein sources could be used instead of FM in aquafeeds. These plants-source proteins may negatively affect feed intake and/or absorption of nutrients due to bad digestion. Therefore, the efficacy of a dietary exogenous enzymes mixture (Hostazyme X; HX) on growth performance, antioxidant capacity, and digestive enzymes in common carp, Cyprinus carpio, was assessed. Dietary HX was included in practical diets at levels of 0.0 (control), 0.1, 0.5, 1.0, and 2.0 g/kg diet. Fish (0.84 ± 0.003 g) were fed on the tested diets up to apparent satiation twice daily in triplicate groups for 8 weeks. The fish performance was significantly improved (P < 0.05) by increasing dietary HX up to 1.0 g/kg diet after which fish growth and feed intake were almost the same. Also, intestinal amylase, lipase, and protease significantly increased (P < 0.05) due to HX supplementation leading to increased feed intake and overall growth performance. Likewise, the antioxidant activity of common carp was stimulated by HX supplementation in a dose-related manner, where the activities of superoxide dismutase, catalase, and glutathione peroxidase were significantly higher (P < 0.05), while malondialdehyde value was significantly lower (P < 0.05) in HX-fed fish groups than those fed the control diet. In conclusion, dietary HX could be used as a feed supplement to confer better growth performance and health of common carp fry with an optimal level of 1.0 g/kg diet.

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References

  • Abdel-Tawwab M, Monier MN (2018) Stimulatory effect of dietary taurine on growth performance, digestive enzymes activity, antioxidant capacity, and tolerance of common carp, Cyprinus carpio L., fry to salinity stress. Fish Physiol Biochem 44:639–649

    Article  CAS  Google Scholar 

  • Abdel-Tawwab M, Khattab YA, Ahmad MH, Shalaby AM (2006) Compensatory growth, feed utilization, whole-body composition, and hematological changes in starved juvenile Nile Tilapia, Oreochromis niloticus (L.). J Appl Aquac 18:17–36

    Article  Google Scholar 

  • Acamovic T (2001) Commercial application of enzyme technology for poultry production. Worlds Poult Sci J 57:225–242

    Article  Google Scholar 

  • Adeoye AA, Yomla R, Jaramillo-Torres A et al (2016) Combined effects of exogenous enzymes and probiotic on Nile tilapia (Oreochromis niloticus) growth, intestinal morphology and microbiome. Aquaculture 463:61–70. https://doi.org/10.1016/j.aquaculture.2016.05.028

    Article  CAS  Google Scholar 

  • Aksnes A, Njaa LR (1981) Catalase, glutathione peroxidase and superoxide dismutase in different fish species. Comp Biochem Physiol B Comp Biochem 69:893–896

    Article  Google Scholar 

  • Ali Zamini A, Kanani HG, Azam Esmaeili A et al (2014) Effects of two dietary exogenous multi-enzyme supplementation, Natuzyme® and beta-mannanase (Hemicell®), on growth and blood parameters of Caspian salmon (Salmo trutta caspius). Comp Clin Pathol 23:187–192. https://doi.org/10.1007/s00580-012-1593-4

    Article  CAS  Google Scholar 

  • AOAC (1995) Association of Official Methods of analytical chemist Official Method Analysis, 16th edn. Arlington, VA, USA

  • Bedford MR, Schulze H (1998) Exogenous enzymes for pigs and poultry. Nutr Res Rev 11:91–114

    Article  CAS  Google Scholar 

  • Ben Ameur W, De Lapuente J, El Megdiche Y, Barhoumi B, Trabelsi S, Camps L, Serret J, Ramos-López D, Gonzalez-Linares J, Driss MR, Borràs M (2012) Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte lagoon (Tunisia). Mar Pollut Bull 64:241–251. https://doi.org/10.1016/j.marpolbul.2011.11.026

    Article  CAS  PubMed  Google Scholar 

  • Bernfeld P (1955) Enzymes of carbohydrate metabolism. Methods Enzymol 1:149–158

    Article  CAS  Google Scholar 

  • Bogut I, Opacak A, Stevic I (1995) The influence of polyzymes added to the food on the growth of carp fingerlings (Cyprinus carpio L.). Aquaculture 129:252

    Article  Google Scholar 

  • Boyd CE, Tucker CS (1998) Pond aquaculture water quality management. Springer Science & Business Media, Berlin

    Book  Google Scholar 

  • Buege JA, Aust SD (2004) [30] Microsomal lipid peroxidation. Methods Enzymol 52:302–310. https://doi.org/10.1016/s0076-6879(78)52032-6

    Article  Google Scholar 

  • Burgos-Aceves MA, Cohen A, Smith Y, Faggio C (2016) Estrogen regulation of gene expression in the teleost fish immune system. Fish Shellfish Immunol 58:42–49. https://doi.org/10.1016/j.fsi.2016.09.006

    Article  CAS  PubMed  Google Scholar 

  • Cahu C, Rønnestad I, Grangier V, Zambonino Infante JL (2004) Expression and activities of pancreatic enzymes in developing sea bass larvae (Dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein; involvement of cholecystokinin. Aquaculture 238:295–308. https://doi.org/10.1016/j.aquaculture.2004.04.013

    Article  CAS  Google Scholar 

  • Carter CG, Houlihan DF, Mccarthy ID (1992) Feed utilization efficiencies of Atlantic salmon (Salmo salar L.) parr: effect of a single supplementary enzyme. Comp Biochem Physiol A Physiol. https://doi.org/10.1016/0300-9629(92)90548-5

    Article  Google Scholar 

  • Carter CG, Houlihan DF, Buchanan B, Mitchell AI (1994) Growth and feed utilization efficiencies of seawater Atlantic salmon, Salmo salar L., fed a diet containing supplementary enzymes. Aquac Res 25:37–46

    Article  Google Scholar 

  • Castillo S, Gatlin DM (2015) Dietary supplementation of exogenous carbohydrase enzymes in fish.pdf. Aquaculture 435:286–292

    Article  CAS  Google Scholar 

  • Chen J, Zhou X, Feng L et al (2009) Effects of glutamine on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var. Jian). Aquaculture 288:285–289. https://doi.org/10.1016/J.AQUACULTURE.2008.10.053

    Article  CAS  Google Scholar 

  • Dawood MAO, Koshio S (2016) Recent advances in the role of probiotics and prebiotics in carp aquaculture: a review. Aquaculture 454:243–251. https://doi.org/10.1016/J.AQUACULTURE.2015.12.033

    Article  CAS  Google Scholar 

  • Dawood M, Mohsen M, El-dakar A et al (2019) Effectiveness of exogenous digestive enzymes supplementation on the performance of rabbitfish (Siganus rivulatus). Slov Vet Res 56:409–419. https://doi.org/10.26873/SVR-779-2019

    Article  Google Scholar 

  • Drew MD, Racz VJ, Gauthier R, Thiessen DL (2005) Effect of adding protease to coextruded flax:pea or canola:pea products on nutrient digestibility and growth performance of rainbow trout (Oncorhynchus mykiss). Anim Feed Sci Technol 119:117–128. https://doi.org/10.1016/j.anifeedsci.2004.10.010

    Article  CAS  Google Scholar 

  • Dytham C (2011) Choosing and using statistics: a biologist’s guide. Wiley, Hoboken

    Google Scholar 

  • FAO (2014) Food and Agriculture Organization of the United Nations. Global Aquaculture Production 1950–2012. FAO, Rome

    Google Scholar 

  • Farhangi M, Carter CG (2007) Effect of enzyme supplementation to dehulled lupin-based diets on growth, feed efficiency, nutrient digestibility and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquac Res 38:1274–1282

    Article  CAS  Google Scholar 

  • Fauconneau B, Cowey CB, Mackie AM, Bell JG (1985) Protein synthesis and protein deposition in fish. Nutrition and feeding in fish, pp 17–46

  • Felix N, Selvaraj S (2004) Enzymes for sustainable aquaculture. Aquacult Asia 9:5–6

    Google Scholar 

  • Francis G, Makkar HPS, Becker K (2001) Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199:197–227

    Article  CAS  Google Scholar 

  • Gatlin DM III, Barrows FT, Brown P et al (2007) Expanding the utilization of sustainable plant products in aquafeeds: a review. Aquac Res 38:551–579

    Article  CAS  Google Scholar 

  • Ghomi MR, Shahriari R, Langroudi HF et al (2012) Effects of exogenous dietary enzyme on growth, body composition, and fatty acid profiles of cultured great sturgeon Huso huso fingerlings. Aquac Int 20:249–254

    Article  CAS  Google Scholar 

  • Gill C (2007) World feed panorama survey. Feed Int 28:5–9

    Google Scholar 

  • Hlophe-Ginindza SN, Moyo NAG, Ngambi JW, Ncube I (2016) The effect of exogenous enzyme supplementation on growth performance and digestive enzyme activities in Oreochromis mossambicus fed kikuyu-based diets. Aquac Res 47:3777–3787

    Article  CAS  Google Scholar 

  • Hoseinifar SH, Ahmadi A, Khalili M et al (2017) The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics. Aquac Res 48:5447–5454. https://doi.org/10.1111/are.13359

    Article  CAS  Google Scholar 

  • Jiang T-T, Feng L, Liu Y et al (2014) Effects of exogenous xylanase supplementation in plant protein-enriched diets on growth performance, intestinal enzyme activities and microflora of juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Nutr 20:632–645

    Article  CAS  Google Scholar 

  • Krajcovicova-Kudlackova M, Ursinyova M, Blazicek P, Spustova V, Ginter E, Hladikova V, Klvanova J (2003) Free radical disease prevention and nutrition. Bratisl Lek Listy 104:64–68

    CAS  PubMed  Google Scholar 

  • Lee JW, Choi YC, Kim R, Lee SK (2015) Multiwall carbon nanotube-induced apoptosis and antioxidant gene expression in the gills, liver, and intestine of Oryzias latipes. Biomed Res Int 2015:1–10. https://doi.org/10.1155/2015/485343

    Article  CAS  Google Scholar 

  • Liew HJ, Fazio A, Faggio C, Blust R, de Boeck G (2015) Cortisol affects metabolic and ionoregulatory responses to a different extent depending on feeding ration in common carp, Cyprinus carpio. Comp Biochem Physiol A Mol Integr Physiol 189:45–57. https://doi.org/10.1016/J.CBPA.2015.07.011

    Article  CAS  PubMed  Google Scholar 

  • Lin S, Mai K, Tan B (2007) Effects of exogenous enzyme supplementation in diets on growth and feed utilization in tilapia, Oreochromis niloticus x O. aureus. Aquac Res 38:1645–1653

    Article  CAS  Google Scholar 

  • Lushchak VI, Bagnyukova TV, Lushchak V et al (2005) Hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (Cyprinus carpio) tissues. Int J Biochem Cell Biol 37:1319–1330

    Article  CAS  Google Scholar 

  • Mallick N, Mohn FH (2000) Reactive oxygen species: response of algal cells. J Plant Physiol 157:183–193

    Article  CAS  Google Scholar 

  • Martínez-Álvarez RM, Morales AE, Sanz A (2005) Antioxidant defenses in fish: biotic and abiotic factors. Rev Fish Biol Fish 15:75–88. https://doi.org/10.1007/s11160-005-7846-4

    Article  Google Scholar 

  • McCord JM, Fridovich I (1969) Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244:6049–6055

    CAS  PubMed  Google Scholar 

  • National Research Council (NRC) (1993) Nutrient requirements of fish. National Academy Press, Washington, DC

  • NRC (2011) Nutrient requirements of fish and shrimp. National academies press

  • Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358

    Article  CAS  Google Scholar 

  • Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  PubMed  Google Scholar 

  • Papada E, Kaliora AC, Gioxari A, Papalois A, Forbes A (2014) Anti-inflammatory effect of elemental diets with different fat composition in experimental colitis. Br J Nutr 111:1213–1220

    Article  CAS  Google Scholar 

  • Ross NW, Firth KJ, Wang A, Burka JF, Johnson SC (2000) Changes in hydrolytic enzyme activities of naive Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation. Dis Aquat Org 41:43–51. https://doi.org/10.3354/dao041043

    Article  CAS  PubMed  Google Scholar 

  • Rzeuski R, Chlubek D, Machoy Z (1998) Interactions between fluoride and biological free radical reactions. Fluoride 31:43–45

    CAS  Google Scholar 

  • Saputra F, Shiu Y-L, Chen Y-C, Puspitasari AW, Danata RH, Liu CH, Hu SY (2016) Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus). Fish & shellfish immunology 58:397–405. https://doi.org/10.1016/j.fsi.2016.09.046

    Article  CAS  Google Scholar 

  • Shihabi ZK, Bishop C (1971) Simplified turbidimetric assay for lipase activity. Clin Chem 17:1150–1153

    Article  CAS  Google Scholar 

  • Soltan MA (2009) Effect of dietary fish meal replacement by poultry by-product meal with different grain source and enzyme supplementation on performance, feces recovery, body composition and nutrient balance of Nile tilapia. Pak J Nutr 8:395–407

    Article  CAS  Google Scholar 

  • Tacon AGJ, Metian M (2009) Fishing for aquaculture: non-food use of small pelagic forage fish—a global perspective. Rev Fish Sci 17:305–317

    Article  Google Scholar 

  • Tacon AGJ, Hasan MR, Metian M (2011) Demand and supply of feed ingredients for farmed fish and crustaceans: trends and prospects. FAO Fisheries and Aquaculture technical paper I

  • Taylor P, Onders RJ (2006) Reservoir ranching of paddlefish, Polyodon spathula : results of a public opinion survey in Kentucky. J Appl Aquac 18:17–36. https://doi.org/10.1300/J028v18n03

    Article  Google Scholar 

  • Vahjen W, Osswald T, Schäfer K, Simon O (2007) Comparison of a xylanase and a complex of non starch polysaccharide-degrading enzymes with regard to performance and bacterial metabolism in weaned piglets. Arch Anim Nutr 61:90–102

    Article  CAS  Google Scholar 

  • Wilhelm DF (1996) Fish antioxidant defenses--a comparative approach. Braz J Med Biol Res Revista brasileira de pesquisas medicas e biologicas 29:1735–1742

    Google Scholar 

  • Wu P, Liu Y, Jiang W-D et al (2017) A comparative study on antioxidant system in fish hepatopancreas and intestine affected by choline deficiency: different change patterns of varied antioxidant enzyme genes and Nrf2 signaling factors. PLoS One 12:e0169888

    Article  Google Scholar 

  • Xiao J, Wang J, Xing F et al (2014) Zeaxanthin dipalmitate therapeutically improves hepatic functions in an alcoholic fatty liver disease model through modulating MAPK pathway. PLoS One 9:e95214

    Article  Google Scholar 

  • Xiao W, Jiang W, Feng L et al (2019) Effect of dietary enzyme-treated soy protein on the immunity and antioxidant status in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Res. https://doi.org/10.1111/are.14016

    Article  CAS  Google Scholar 

  • Ye J, Wang K, Li F, Sun Y (2011) Single or combined effects of fructo-and mannan oligosaccharide supplements and Bacillus clausii on the growth, feed utilization, body composition, digestive enzyme activity, innate immune response and lipid metabolism of the Japanese flounder Paralichthy. Aquac Nutr 17:e902–e911

    Article  Google Scholar 

  • Yildirim YB, Turan F (2010) Effects of exogenous enzyme supplementation in diets on growth and feed utilization in African catfish, Clarias gariepinus. J Anim Vet Adv 9:327–331

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to express their gratitude to the director, NOREL-Misr Animal Nutrition, Egypt for providing Hostazyme X used in the present work. The author thanks Prof. Mohsen Abdel-Tawwab, Department of Fish Biology and Ecology, Central Laboratory for Aquaculture Research, Agricultural Research Center for his help during the running of this study.

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  1. Department of Fish Biology and Ecology, Central Laboratory for Aquaculture Research, Agricultural Research Center, Abbassa, Abo-Hammad, Sharqia, Egypt

    Mohamed N. Monier

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Correspondence to Mohamed N. Monier.

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Monier, M.N. Efficacy of dietary exogenous enzyme supplementation on growth performance, antioxidant activity, and digestive enzymes of common carp (Cyprinus carpio) fry. Fish Physiol Biochem 46, 713–723 (2020). https://doi.org/10.1007/s10695-019-00745-z

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