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Protection of teprenone against hypoxia and reoxygenation stress in stomach and intestine of Lateolabrax maculatus

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Abstract

Teprenone (geranylgeranylacetone) is one kind of safe and effective agent in gastrointestinal mucosa, which have been widely used in human and veterinary, but rarely used in aquaculture animals. In this study, Lateolabrax maculatus, an important economic fish species in southern China, was taken as the object of study to investigate the protective effect of teprenone on intestinal stress. The present study was designed to investigate the potential mechanism underlying the protection offered by teprenone to protect the gastrointestinal tract against hypoxia and reoxygenation injury of L. maculatus. (a) For oxidative stress parameters, SOD, CAT, and T-AOC in control group were higher than those in teprenone group. MDA content was significantly higher than that in teprenone group at N and 12h time points in intestine (P < 0.05), and at 12, 24, and 48 h time points in stomach. (b) For immune-associated proteins, LZM activity in the control group was lower than that in the teprenone group, and the difference between the two groups in stomach and intestine was significant at 12.48 h and 6.48 h time points, respectively (P < 0.05). Compared with time point N, the content of HSP70 in the control group increased at 0 h in intestine. At 0–48 h, intestine HSP70 content in the control group showed a gradually decreasing trend, which was higher than that in the teprenone group. (c) For apoptosis-related factors, the activity of Cyt-C, caspase9, and caspase3 increased first and then decreased in both groups. The content of Cyt-C in the control group was significantly higher than that in the teprenone group at N-3.6 h, and 3.48 h time points in stomach and intestine, respectively (P<0.05). The activity of caspase9 and caspase3 was higher than that in the teprenone group at N-48 h. Results indicated that acute hypoxia and reoxygenation cause the expression levels of oxidative stress and apoptosis-related factors in the stomach and intestine increased first and then decreased within 0–48 h. Acute hypoxia and reoxygenation also that causes the level of nonspecific immunity decreased first and then increased. A total of 400-mg/kg treatment of teprenone can protect stomach and intestinal tissues to a certain extent. It can effectively protect oxidative stress and apoptosis within 0–48 h after acute hypoxia and reoxygenation and enhance non-specific immunity.

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Acknowledgments

The authors are grateful to all laboratory members for the experimental material preparation.

Funding

This study was supported by Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS (2017YB15), Central Public-interest Scientific Institution Basal Research Fund, CAFS (2019XT0403), and the Central Government directs Special Funds for Local Science and Technology Development (Z135050009017). Project supported by Technology projects of Yangzhou City, Jiangsu province, China (YZ2018006). The experiments comply with current laws of China.

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  1. YongXu Sun and HongBiao Dong equally contributed to the work.

Authors and Affiliations

  1. Key Lab. of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China

    YongXu Sun, HongBiao Dong, WenHao Wang, YaFei Duan, Mujiao Xie, QingSong Liu, Hua Li & JiaSong Zhang

  2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    YongXu Sun & WenHao Wang

  3. Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518001, China

    AiJun Zhan

  4. Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, China

    JiaSong Zhang

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Sun, Y., Dong, H., Zhan, A. et al. Protection of teprenone against hypoxia and reoxygenation stress in stomach and intestine of Lateolabrax maculatus. Fish Physiol Biochem 46, 575–584 (2020). https://doi.org/10.1007/s10695-019-00732-4

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