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Influence of dietary fermented Saccharomyces cerevisiae on growth performance, oxidative stress parameters, and immune response of cultured Oreochromis niloticus

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Abstract

Our trial was performed to investigate the effect of fully fermented yeast Sacharomyces cerevisiae (Hilyses, ICC Company, Brazil) on the growth performance and immune response of Oreochromis niloticus. In this study, a total of 270 O. niloticus (50.7 ± 0.8 g) were randomly divided into 3 groups in triplicates. The control group was fed on the basal diet while the other two groups were fed on a basal diet supplemented with 0.2% and 0.4% of Hilyses. The trial period extended for 2 months. At the end of the feeding trial, oxidant and antioxidant parameters (MDA, catalase, and glutathione reductase), some innate immunological parameters and immune-related gene expression were measured. Histological examination of liver, spleen, kidney, and intestine was performed. Further, fish groups were challenged against Gram-negative and Gram-positive bacteria; A. hydrophila and L. garvieae. The results revealed significant improvement (p < 0.05) in growth performance and feed utilization in Hilyses-treated groups versus the control group. Blood parameters and liver and kidney functions of Hilyses-supplemented groups were similar to those of the control group. The histological findings of treated groups showed normal tissue structure with multiple focal lymphoid aggregations in the spleen, kidney, and intestine. Both levels of Hilyses successfully enhanced phagocytic activity/index, lysozyme activity, and gene expression of TNF-α, and IL-1β. Fish group fed on 0.4% Hilyses exhibited the highest expression of IL-1β and the least mortality percentages post challenges. Thus, dietary supplementation of Hilyses could promote the growth performance and immunity and increase the resistance of O. niloticus against diseases.

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Authors and Affiliations

  1. Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Nermeen M. Abu-Elala & Nehal A. Younis

  2. Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Huda O. AbuBakr

  3. Department of nutrition and clinical nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Naela M. Ragaa

  4. Research and Development Department, ICC Industrial Comércio Exportação e Importação Ltda., São Paulo, 01451-909, Brazil

    Liliana L. Borges & Melina A. Bonato

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  1. Nermeen M. Abu-Elala
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  2. Nehal A. Younis
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  3. Huda O. AbuBakr
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  4. Naela M. Ragaa
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  5. Liliana L. Borges
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  6. Melina A. Bonato
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Contributions

NMA-E and NAY contributed to the design of the experimental study, growth performance, haematological, immune response evaluation, and challenge test. HOAB determined the oxidant and antioxidant parameters and molecular and gene expression study. NMR contributed to the experimental diet formulation. LLB and MAB supply the study with Hilyses® product. All authors read and revised the manuscript.

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Correspondence to Nermeen M. Abu-Elala.

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The research was conducted in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals approved by the Institutional Animal Use and Care Committee (IACUC), Cairo University, Giza, Egypt. All authors have reviewed the manuscript and approved its submission for publication.

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Abu-Elala, N.M., Younis, N.A., AbuBakr, H.O. et al. Influence of dietary fermented Saccharomyces cerevisiae on growth performance, oxidative stress parameters, and immune response of cultured Oreochromis niloticus. Fish Physiol Biochem 46, 533–545 (2020). https://doi.org/10.1007/s10695-019-00711-9

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