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Effects of temperature on the transcriptomes of pituitary and liver in Golden Pompano Trachinotus blochii

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Abstract

Fish growth can be modulated dynamically through the brain-pituitary-liver regulation axis. In the present study, whole transcriptomes of the pituitary and liver from Golden Pompano Trachinotus blochii were sequenced in seawater at 20 °C (T_low) and 25 °C (T_high). A total of 187,277,583 paired-end reads were assembled to obtain 100,495 transcripts, corresponding to 83,974 genes. These reads were mapped to T. blochii transcripts, and the mapping accuracy ranged from 80.4 to 94.9%. Two lists of differentially expressed genes were obtained by comparisons of pituitary and liver T_low versus T_high groups, comprising 458 and 205 genes, respectively. Of these, 33 differentially expressed genes were common between the two lists. Twelve GO terms were overrepresented for the 458 differentially expressed genes in the pituitary, and it is noteworthy that the GO term galanin receptor activity (GO: 0004966) related to the modulation of appetite and metabolism, whose genes made up half of all assembled genes in the term. For the 205 differentially expressed genes in the liver, 19 overrepresented GO terms were mainly related to immune regulation, digestion, and protein metabolism. Among the common differentially expressed genes, there were 32 genes that had identical changing trends in both pituitary and liver comparisons. Furthermore, two GO terms inorganic diphosphatase activity and MHC protein complex were overrepresented. These results indicate that the brain could regulate pituitary function through galanin signal mechanism and that the metabolism of liver was further optimized to modulate immunity and growth under different temperatures.

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Acknowledgements

The authors were grateful to all the laboratory members for continuous technical advice and helpful discussion.

Funding

This work was supported by the National Natural Science Foundation of China (NO.31660737), the National Key Research and Development Program of China (No.2018YFD0900704), and Scientific Research Foundation of Hainan University (kyqd1554).

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Authors and Affiliations

  1. State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, 58 Renmin Road, Haikou, 570228, People’s Republic of China

    Zhi Zhou, Yanqiang Li, Guoqing Zhang, Hengzhen Ye & Jian Luo

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  1. Zhi Zhou
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Correspondence to Jian Luo.

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Zhou, Z., Li, Y., Zhang, G. et al. Effects of temperature on the transcriptomes of pituitary and liver in Golden Pompano Trachinotus blochii. Fish Physiol Biochem 46, 63–73 (2020). https://doi.org/10.1007/s10695-019-00695-6

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