Fish Physiology and Biochemistry

, Volume 44, Issue 4, pp 1087–1097 | Cite as

Effects of dietary organic, inorganic, and nanoparticulate selenium sources on growth, hemato-immunological, and serum biochemical parameters of common carp (Cyprinus carpio)

  • Sadegh Saffari
  • Saeed Keyvanshokooh
  • Mohammad Zakeri
  • Seyed Ali Johari
  • Hossein Pasha-Zanoosi
  • Mansour Torfi Mozanzadeh


An 8-week feeding trial was conducted to compare the effects of supplementing (0.7 mg kg−1) different dietary selenium (Se) sources including organic [selenomethionine (SeMet)], inorganic [sodium selenite (Na2SeO3)], and nanoparticulate Se (nano-Se) on physiological responses of common carp, Cyprinus carpio juveniles (9.7 ± 0.1 g). Basal diet without Se supplementation used as control. Fish fed nano-Se supplemented diet had the highest weight gain (97.2 ± 10.8%) and feed efficiency ratio (42.4 ± 0.8%). Intestinal villi height was significantly taller in fish fed nano-Se diet than in the control group in both foregut and midgut sections. Serum glutathione peroxidase and superoxide dismutase activities were significantly higher in nano-Se and SeMet groups than in control and sodium selenite groups. Fish fed Se-supplemented diets had greater red blood cell counts and hematocrit and hemoglobin values than the control group (P < 0.05). Nano-Se and SeMet groups showed a significant increase in white blood cell counts, neutrophil percentage, and serum lysozyme activity than the other groups. Fish fed nano-Se diet had the highest serum hemolytic activity, total immunoglobulin, and total protein and albumin contents, as well as the lowest serum total cholesterol and low density lipoprotein levels (P < 0.05). Overall, significant improvements in growth performance, feed utilization, intestinal morphology, and hemato-immunological and serum biochemical parameters of common carp juveniles suggest nano-Se as an efficient source for providing dietary Se in this species.


Selenium Antioxidant enzymes Intestinal villus Immune response Serum biochemical parameters 


Funding information

This work was funded by Khorramshahr University of Marine Science and Technology (Grant No. 222945).


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Sadegh Saffari
    • 1
  • Saeed Keyvanshokooh
    • 1
  • Mohammad Zakeri
    • 1
  • Seyed Ali Johari
    • 2
  • Hossein Pasha-Zanoosi
    • 3
  • Mansour Torfi Mozanzadeh
    • 4
  1. 1.Department of Fisheries, Faculty of Marine Natural ResourcesKhorramshahr University of Marine Science and TechnologyKhorramshahrIran
  2. 2.Department of Fisheries, Faculty of Natural ResourcesSanandajIran
  3. 3.Department of Physical Oceanography, Faculty of Marine SciencesKhorramshahr University of Marine Science and TechnologyKhorramshahrIran
  4. 4.South Iran Aquaculture Research CentreAhwazIran

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