Fish Physiology and Biochemistry

, Volume 44, Issue 3, pp 817–828 | Cite as

Effects of temperature and melatonin on day–night expression patterns of arginine vasotocin and isotocin mRNA in the diencephalon of a temperate wrasse Halichoeres tenuispinis

  • Selma Bouchekioua
  • Sung-Pyo Hur
  • Yuki Takeuchi
  • Young-Don Lee
  • Akihiro Takemura
Article

Abstract

Most wrasses are protogynous species that swim to feed, reproduce during the daytime, and bury themselves under the sandy bottom at night. In temperate and subtropical wrasses, low temperature influences emergence from the sandy bottom in the morning, and induces a hibernation-like state in winter. We cloned and characterized the prohormone complementary DNAs (cDNAs) of arginine vasotocin (AVT) and isotocin (IT) in a temperate wrasse (Halichoeres tenuispinis) and examined the effects of day/night and temperature on their expression in the diencephalon, because these neurohypophysial peptides are related to the sex behavior of wrasses. The full-length cDNAs of pro-AVT and pro-IT were 938 base pairs (154 amino acids) and 759 base pairs (156 amino acids) in length, respectively. Both pro-peptides contained a signal sequence followed by the respective hormones and neurophysin connected by a Gly–Lys–Arg bridge. Reverse-transcription polymerase chain reaction (RT-PCR) revealed that pro-AVT mRNA expression was specifically observed in the diencephalon, whereas pro-IT mRNA expression was seen in the whole brain. Quantitative RT-PCR revealed that the mRNA abundance of pro-AVT and pro-IT was higher at midday (zeitgeber time 6; ZT6) than at midnight (ZT18) under 12 h light and 12 h darkness (LD 12:12) conditions, but not under constant light. Intraperitoneal injection of melatonin decreased the mRNA abundance of pro-AVT, but not of pro-IT. When fish were reared under LD 12:12 conditions at 25, 20, and 15 °C, day high and night low mRNA expressions of pro-AVT and pro-IT were maintained. A field survey revealed seasonal variation in the number of swimming fish at observatory sites; many fish emerged from the sandy bottom in summer, but not in winter, suggesting a hibernation-like state under the sandy bottom under low temperature conditions. We conclude that the day–night fluctuation of pro-AVT and pro-IT mRNA abundance in the brain is not affected by temperature and repeated under the sandy bottom in winter.

Keywords

Arginine vasotocin Circadian Hibernation Isotocin Melatonin qPCR Temperature 

Notes

Funding

This study was supported in part by a Grant-in-Aid for Scientific Research (B) (KAKENHI, grant number 16H05796) from the Japan Society for the Promotion of Science (JSPS) to AT and Heiwa Nakajima Foundation to AT, and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A6A3A04041089) to SPH.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemistry, Biology and Marine Science, Faculty of ScienceUniversity of the RyukyusOkinawaJapan
  2. 2.Jeju International Marine Science Research & Education CenterKorea Institute of Ocean Science & TechnologyJeju Special Self-Governing ProvinceSouth Korea
  3. 3.Developmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate UniversityOnna-sonJapan
  4. 4.Marine Science InstituteJeju National UniversityJeju Special Self-Governing ProvinceSouth Korea

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