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Growth performance and protective effect of vitamin E on oxidative stress pufferfish (Takifugu obscurus) following by ammonia stress

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Abstract

This study was conducted to determine the effects of vitamin E on growth performance, biochemical parameters, and antioxidant capacity of pufferfish (Takifugu obscurus) exposed to ammonia stress. The experimental basal diets supplemented with vitamin E at the rates of 2.31 (control), 21.84, 40.23, 83.64, 158.93, and 311.64 mg kg−1 dry weight were fed to fish for 60 days. After the feeding trial, the fish were exposed to 100 mg L−1 ammonia-nitrogen for 48 h. The results shown that the vitamin E group significantly improved weight gain, specific growth rate, and the expression levels of growth hormone receptors and insulin-like growth factor. Fish fed with the vitamin E-supplemented diets could increase plasma alkaline phosphatase activities and decrease plasma glutamicoxalacetic transaminase and glutamic-pyruvic transaminase activities. The relative expression levels of heat shock proteins (40.23–311.64 mg kg−1 vitamin E diet group), manganese superoxide dismutase (83.64–158.93 mg kg−1 vitamin E diet group), catalase (40.23–311.64 mg kg−1 vitamin E diet group), and glutathione reductase (40.23–311.64 mg kg−1 vitamin E diet group) were upregulated. On the other hand, the decreased level of reactive oxygen species (ROS) was observed in the 83.64–311.64 mg kg−1 vitamin E additive group. These results showed that vitamin E might have a potentially useful role as an effective antioxidant to improve resistance in pufferfish.

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Acknowledgements

This research was supported by Hainan Applied Technology Research and Development and Demonstration Projects (ZDXM2015025).

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Authors and Affiliations

  1. Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, Guangdong, People’s Republic of China

    Chang-Hong Cheng & Zhi-Xun Guo

  2. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center (SCS-REPIC), Guangzhou, People’s Republic of China

    Zhi-Xun Guo

  3. Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou, 510631, People’s Republic of China

    An-Li Wang

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  1. Chang-Hong Cheng
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  2. Zhi-Xun Guo
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Correspondence to Chang-Hong Cheng, Zhi-Xun Guo or An-Li Wang.

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Cheng, CH., Guo, ZX. & Wang, AL. Growth performance and protective effect of vitamin E on oxidative stress pufferfish (Takifugu obscurus) following by ammonia stress. Fish Physiol Biochem 44, 735–745 (2018). https://doi.org/10.1007/s10695-018-0468-2

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