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Fish Physiology and Biochemistry

, Volume 44, Issue 2, pp 735–745 | Cite as

Growth performance and protective effect of vitamin E on oxidative stress pufferfish (Takifugu obscurus) following by ammonia stress

  • Chang-Hong Cheng
  • Zhi-Xun Guo
  • An-Li Wang
Article

Abstract

This study was conducted to determine the effects of vitamin E on growth performance, biochemical parameters, and antioxidant capacity of pufferfish (Takifugu obscurus) exposed to ammonia stress. The experimental basal diets supplemented with vitamin E at the rates of 2.31 (control), 21.84, 40.23, 83.64, 158.93, and 311.64 mg kg−1 dry weight were fed to fish for 60 days. After the feeding trial, the fish were exposed to 100 mg L−1 ammonia-nitrogen for 48 h. The results shown that the vitamin E group significantly improved weight gain, specific growth rate, and the expression levels of growth hormone receptors and insulin-like growth factor. Fish fed with the vitamin E-supplemented diets could increase plasma alkaline phosphatase activities and decrease plasma glutamicoxalacetic transaminase and glutamic-pyruvic transaminase activities. The relative expression levels of heat shock proteins (40.23–311.64 mg kg−1 vitamin E diet group), manganese superoxide dismutase (83.64–158.93 mg kg−1 vitamin E diet group), catalase (40.23–311.64 mg kg−1 vitamin E diet group), and glutathione reductase (40.23–311.64 mg kg−1 vitamin E diet group) were upregulated. On the other hand, the decreased level of reactive oxygen species (ROS) was observed in the 83.64–311.64 mg kg−1 vitamin E additive group. These results showed that vitamin E might have a potentially useful role as an effective antioxidant to improve resistance in pufferfish.

Keywords

Vitamin E Growth performance Oxidative stress Disease resistance 

Notes

Acknowledgements

This research was supported by Hainan Applied Technology Research and Development and Demonstration Projects (ZDXM2015025).

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouPeople’s Republic of China
  2. 2.South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center (SCS-REPIC)GuangzhouPeople’s Republic of China
  3. 3.Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life ScienceSouth China Normal UniversityGuangzhouPeople’s Republic of China

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