Fish Physiology and Biochemistry

, Volume 44, Issue 2, pp 651–659 | Cite as

Duplication and gene expression patterns of β-catenin in Nile tilapia

  • Fengrui Wu
  • Limin Wu
  • Qingqing Wu
  • Linyan Zhou
  • Wenyong Li
  • Deshou Wang
Article

Abstract

β-Catenin, a key transcriptional coactivator of the Wnt pathway, plays an important role in animal embryonic development and organogenesis. In our earlier study, we have reported that two types of β-catenin (β-catenin-1 and β-catenin-2) were ubiquitously expressed in almost all the tissues examined in tilapia. However, the immunolocalization of β-catenin in those tissues, especially in extra-gonadal tissues, remains unclear. In the present study, we further confirm that these two types of β-catenin gene exist only in teleosts and are derived from 3R (third round of genome duplication) by phylogenetic and syntenic analyses. Moreover, the transcriptome analysis conducted in this investigation reveals that two β-catenins exhibited similar expression patterns in seven adult tissues and four key developmental stages of XX and XY gonads. Finally, immunohistochemistry analysis was performed to detect the cell localization of β-catenin. A positive signal of β-catenin was observed in various tissues of tilapia, such as the intestine, liver, kidney, spleen, eye, brain, and gonads. The results of our study indicate that tilapia β-catenin might be involved in the organ development and play some specific functions in biological processes; all these data will provide basic reference for understanding the molecular mechanism of β-catenin in regulating of teleost organogenesis.

Keywords

β-Catenin Expression Immunolocalization Tilapia 

Notes

Compliance with ethical standards

All animal experiments conformed to the Guide for Care and Use of Laboratory Animals and were approved by the Institutional Committee of Laboratory Animal Experimentation at the Southwest University in China.

Supplementary material

10695_2017_460_MOESM1_ESM.doc (296 kb)
ESM 1 (DOC 296 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Fengrui Wu
    • 1
    • 2
  • Limin Wu
    • 2
    • 3
  • Qingqing Wu
    • 1
  • Linyan Zhou
    • 2
  • Wenyong Li
    • 1
  • Deshou Wang
    • 2
  1. 1.Key Laboratory of Embryo Development and Reproductive Regulation, School of Biological and Food EngineeringFuyang Teachers CollegeFuyangChina
  2. 2.Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life ScienceSouthwest UniversityChongqingChina
  3. 3.College of FisheriesHenan Normal UniversityXinxiangChina

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