Duplication and gene expression patterns of β-catenin in Nile tilapia
β-Catenin, a key transcriptional coactivator of the Wnt pathway, plays an important role in animal embryonic development and organogenesis. In our earlier study, we have reported that two types of β-catenin (β-catenin-1 and β-catenin-2) were ubiquitously expressed in almost all the tissues examined in tilapia. However, the immunolocalization of β-catenin in those tissues, especially in extra-gonadal tissues, remains unclear. In the present study, we further confirm that these two types of β-catenin gene exist only in teleosts and are derived from 3R (third round of genome duplication) by phylogenetic and syntenic analyses. Moreover, the transcriptome analysis conducted in this investigation reveals that two β-catenins exhibited similar expression patterns in seven adult tissues and four key developmental stages of XX and XY gonads. Finally, immunohistochemistry analysis was performed to detect the cell localization of β-catenin. A positive signal of β-catenin was observed in various tissues of tilapia, such as the intestine, liver, kidney, spleen, eye, brain, and gonads. The results of our study indicate that tilapia β-catenin might be involved in the organ development and play some specific functions in biological processes; all these data will provide basic reference for understanding the molecular mechanism of β-catenin in regulating of teleost organogenesis.
Keywordsβ-Catenin Expression Immunolocalization Tilapia
This work was supported by grants from the National Natural Science Foundation of China (No. 31501906), the Key Grant of the Key Projects of the Outstanding Young Talents in Colleges and Universities of Anhui Province (No. gxyqZD201619), and the Major Project of Discipline Construction in Anhui Province and Anhui Provincial Natural Science Foundation (No. 1408085QC65).
Compliance with ethical standards
All animal experiments conformed to the Guide for Care and Use of Laboratory Animals and were approved by the Institutional Committee of Laboratory Animal Experimentation at the Southwest University in China.
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