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Preliminary studies on haematological and plasmatic parameters in gilthead sea bream (Sparus aurata) held under day/night temperature variations

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Abstract

This study aimed to evaluate to what extent diel water temperature oscillations over a short period of time (41 h) affected haematologic and plasmatic parameters of gilthead sea bream. Sea bream juveniles (160 fish; 177.2 ± 20.9 g) were divided in two homogenous groups, experimental and control. The experimental group was exposed to water at 22.3 ± 0.6 °C between 5 a.m. and 5 p.m. and to colder water from 5 p.m. to 5 a.m. (temperature variation of 8.6 ± 0.3 °C) during 41h period, whereas for the control group, water temperature was maintained constant (22.4 ± 0.7 °C) for the same period. Fish were fed three times per day (10:30 a.m., 2 p.m., 4 p.m.). Blood was collected from fish caudal veins (n = 6) at different time points (10 a.m., 1 p.m., 5 p.m., 6:30 p.m., 8 p.m. and 10 a.m. of the following day) from each group, for haematologic (haematocrit and haemoglobin) and plasmatic (glucose, cortisol, triglycerides and total protein) determinations. All parameters studied tended to vary throughout the study period, both for experimental and control groups. Significant differences were observed between the two groups for all the studied parameters at different sampling points. These differences were observed near the influence of the cold-water period or during the cooling-water period. Results suggested that colder water temperature cycles depressed fish metabolic activity and affected the levels of the analysed blood-circulating metabolites. Overall, results suggested that stress response was similar for experimental and control groups and that gilthead sea bream were able to cope with diel water temperature variations.

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References

  • Adeyemo OK, Agbede SA, Olaniyan AO, Shoaga OA (2003) The haematological response of Clarias gariepinus to changes in acclimation temperature. Afr J Biomed Res 6:105–108. https://doi.org/10.4314/ajbr.v6i2.54033

    Google Scholar 

  • Barton BA, Morgan JD, Vijayan MM (2002) Physiological and condition-related indicators of environmental stress in fish, Chapter 4. In: Adams SM (ed) Biological indicators of aquatic ecosystem stress. American Fisheries Society, Bethesda, pp 111–148

    Google Scholar 

  • Basurco B, Lovatelli A, García B (2011) Current status of sparidae aquaculture, Chapter 1. In: Sparidae: biology and aquaculture of gilthead sea bream and other species. Wiley-Blackwell, pp 1–50. https://doi.org/10.1002/9781444392210.ch1

  • Cardia F, Lovatelli A (2007) A review of cage aquaculture: Mediterranean Sea, Chapter 498. In: Halwart M (ed) Cage aquaculture—regional reviews and global overview. FAO Fisheries Technical Paper, Rome, pp 157–187

  • Cerdá-Reverter JM, Zanuy S, Carrillo M, Madrid JA (1998) Time-course studies on plasma glucose, insulin and cortisol in sea bass (Dicentrarchus labrax) held under different photoperiodic regimes. Physiol Behav 64:245–250. https://doi.org/10.1016/S0031-9384(98)00048-1

    Article  PubMed  Google Scholar 

  • Chen C-Y, Wooster GA, Bowser PR (2004) Comparative blood chemistry and histopathology of tilapia infected with Vibrio vulnificus or Streptococcus iniae or exposed to carbon tetrachloride, gentamicin, or copper sulphate. Aquaculture 239:421–443. https://doi.org/10.1016/j.aquaculture.2004.05.033

    Article  CAS  Google Scholar 

  • Cousineau A, Midwood JD, Stamplecoskie K, King G, Suski CD, Cooke SJ (2014) Diel patterns of baseline glucocorticoids and stress responsiveness in a teleost fish (bluegill, Lepomis macrochirus). Can J Zool 92:417–421. https://doi.org/10.1139/cjz-2014-0054

    Article  CAS  Google Scholar 

  • Drabkin DL, Austin JH (1935) Spectrophotometric studies: II. Preparations from washed blood cells: nitric oxide haemoglobin and sulfhemoglobin. J Biol Chem 112:51–65

    CAS  Google Scholar 

  • Eslamloo K, Akhavan SR, Fallah FJ, Henry MA (2014) Variations of physiological and innate immunological responses in goldfish (Carassius auratus) subjected to recurrent acute stress. Fish Shellfish Immunol 37:147–153. https://doi.org/10.1016/j.fsi.2014.01.014

    Article  CAS  PubMed  Google Scholar 

  • Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751–766

    CAS  PubMed  Google Scholar 

  • Ibarz A, Blasco J, Sala-Rabanal M, Gallardo Á, Redondo A, Fernández-Borràs J (2007) Metabolic rate and tissue reserves in githead sea bream (Sparus aurata) under thermal fluctuations and fasting and their capacity for recovery. Can J Fish Aquat Sci 64:1034–1042. https://doi.org/10.1139/f07-079

    Article  CAS  Google Scholar 

  • Ibarz A, Padrós F, Gallardo MÁ, Fernández-Borràs J, Blasco J, Tort L (2010) Low-temperature challenges to gilthead sea bream culture: review of cold-induced alterations and “winter syndrome”. Rev Fish Biol Fish 20:539–556. https://doi.org/10.1007/s11160-010-9159-5

    Article  Google Scholar 

  • Jobling M, Peruzzi S (2010) Seabreams and porgies (family: Sparidae), Chapter 16. In: Le François N, Jobling M, Carter C, Blier P (eds) Finfish aquaculture diversification. CABI, CAB International, Oxfordshire, pp 361–373. https://doi.org/10.1079/9781845934941.0361

    Chapter  Google Scholar 

  • Johnston IA, Dunn J (1987) Temperature acclimation and metabolism in ectotherms with particular reference to teleost fish. Symp Soc Exp Biol 41:67–93

    CAS  PubMed  Google Scholar 

  • Kyprianou T-D, Pörtner HO, Anestis A, Kostoglou B, Feidantsis K, Michaelidis B (2010) Metabolic and molecular stress responses of gilthead sea bream Sparus aurata during exposure to low ambient temperature: an analysis of mechanisms underlying the winter syndrome. J Comp Physiol B 180:1005–1018. https://doi.org/10.1007/s00360-010-0481-y

    Article  CAS  PubMed  Google Scholar 

  • López-Olmeda JF, Montoya A, Oliveira C, Sánchez-Vázquez J (2009) Synchronization to light and restricted-feeding schedules of behavioral and humoral daily rhythms in gilthead sea bream (Sparus aurata). Chronobiol Int 26:1389–1408. https://doi.org/10.3109/07420520903421922

    Article  PubMed  Google Scholar 

  • Martínez-Porchas M, Martínez-Córdova LR, Ramos-Enriquez R (2009) Cortisol and glucose: reliable indicators of fish stress? Panam J Aquat Sci 4:158–178

    Google Scholar 

  • Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation. Rev Fish Biol Fish 9:211–268. https://doi.org/10.1023/A:1008924418720

    Article  Google Scholar 

  • Montoya A, López-Olmeda JF, Garayzar ABS, Sánchez-Vázquez FJ (2010) Synchronization of daily rhythms of locomotor activity and plasma glucose, cortisol and thyroid hormones to feeding in gilthead seabream (Sparus aurata) under a light-dark cycle. Physiol Behav 101:101–107. https://doi.org/10.1016/j.physbeh.2010.04.019

    Article  CAS  PubMed  Google Scholar 

  • Olsen RE, Sundell K, Ringø E, Myklebust R, Hemre G-I, Hansen T, Karlsen Ø (2008) The acute stress response in fed and food deprived Atlantic cod, Gadus morhua L. Aquaculture 280:232–241. https://doi.org/10.1016/j.aquaculture.2008.05.006

    Article  Google Scholar 

  • Ortuño J, Esteban MA, Meseguer J (2002) Lack of effect of combining different stressors on innate immune responses of seabream (Sparus aurata L.) Vet Immunol Immunopathol 84:17–27. https://doi.org/10.1016/S0165-2427(01)00387-7

    Article  PubMed  Google Scholar 

  • Pavlidis M, Berry M, Divanach P, Kentouri M (1997) Diel pattern of haematocrit, serum metabolites, osmotic pressure, electrolytes and thyroid hormones in sea bass and sea bream. Aquac Int 5:237–247. https://doi.org/10.1023/A:1018391418801

    Article  Google Scholar 

  • Peres H, Santos S, Oliva-teles A (2013) Selected plasma biochemistry parameters in gilthead seabream (Sparus aurata) juveniles. J Appl Ichthyol 29:630–636. https://doi.org/10.1111/j.1439-0426.2012.02049.x

    Article  CAS  Google Scholar 

  • Polakof S, Ceinos RM, Fernández-Durán B, Míguez JM, Soengas JL (2007) Daily changes in parameters of energy metabolism in brain of rainbow trout: dependence on feeding. Comp Biochem Physiol A 146:265–273. https://doi.org/10.1016/j.cbpa.2006.10.026

    Article  Google Scholar 

  • Pousão-Ferreira P, Gonçalves CC, Dores E (2005) Larval rearing of four Sparidae species. Book of short comunications. European Aquaculture Society. Special Publication, n°36. pp 412–415. Proceedings of Larvi 2005. 4th Fish and Shellfish Larviculture Symposium. Gent, Belgium, 5–8 Setembro 2005

  • Ribeiro L, Moura J, Santos M, Colen R, Rodrigues V, Bandarra N, Soares F, Ramalho P, Barata M, Moura P, Pousão-Ferreira P, Dias J (2015) Effect of vegetable based diets on growth, intestinal morpholy, activity of intestinal enzymes and haematological stress indicators in meagre (Argyrosomus regius). Aquaculture 447:116–128. https://doi.org/10.1016/j.aquaculture.2014.12.017

    Article  CAS  Google Scholar 

  • Sala-Rabanal M, Sánchez J, Ibarz A, Fernández-Borràs J, Blasco J, Gallardo MA (2003) Effects of low temperatures and fasting on hematology and plasma composition of gilthead seabream (Sparus aurata). Fish Physiol Biochem 29:105–115. https://doi.org/10.1023/B:FISH.0000035904.16686.b6

    Article  CAS  Google Scholar 

  • Sangiao-Alvarellos S, Guzmán JM, Laíz-Carríon R, Míguez JM, Martín Del Río MP, Mancera JM, Soengas JL (2005) Interactive effects of high stocking density and food deprivation on carbohydrate metabolism in several tissues of gilthead sea bream Sparus auratus. J Exp Zool 303A:761–775. https://doi.org/10.1002/jez.a.203

    Article  Google Scholar 

  • Suski CD, Cooke SJ, Danylchuk AJ, O’Connor CM, Gravel M-A, Redpath T, Hanson KC, Gingerich AJ, Murchie KJ, Danylchuk SE, Koppelman JB, Goldberg TL (2007) Physiological disturbance and recovery dynamics of bonefish (Albula vulpes), a tropical marine fish, in response to variable exercise and exposure to air. Comp Biochem Physiol A 148:664–673. https://doi.org/10.1016/j.cbpa.2007.08.018

    Article  Google Scholar 

  • Vera LM, Montoya A, Pujante IM, Pérez-Sánchez J, Calduch-Giner JA, Mancera JM, Moliner J, Sánchez-Vázquez FJ (2014) Acute stress response in gilthead sea bream (Sparus aurata L.) is time-of-day dependent: physiological and oxidative stress indicators. Chronobiol Int 31:1051–1061. https://doi.org/10.3109/07420528.2014.945646

    Article  PubMed  Google Scholar 

  • Wells RMG, Pankhurst NW (1999) Evaluation of simple instruments for the measurement of blood glucose and lactate, and plasma protein as stress indicators in fish. J World Aquacult Soc 30:276–284. https://doi.org/10.1111/j.1749-7345.1999.tb00876.x

    Article  Google Scholar 

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Funding

This work was supported by AQUAENERG (PROMAR 31-03-05-FEP-0076) and DIVERSIAQUA (Mar2020 16-02-01-FMP-0066). ACM was supported by a post-doctoral grant from FCT (SFRH/BPD/117504/2016).

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Authors and Affiliations

  1. Aquaculture Research Station (EPPO), Portuguese Institute for the Ocean and Atmosphere (IPMA), Parque Natural da Ria Formosa s/n, 8700-194, Olhão, Portugal

    Ana C. Matias, Laura Ribeiro, Ravi L. Araujo & Pedro Pousão-Ferreira

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  1. Ana C. Matias
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  2. Laura Ribeiro
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  3. Ravi L. Araujo
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  4. Pedro Pousão-Ferreira
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Corresponding author

Correspondence to Laura Ribeiro.

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All fish manipulations were directed and carried out by trained scientists (following category C FELASA recommendations) and were conducted according to the European guidelines on protection of animal used for scientific purposes (Directive 2010/63/UE of European Parliament and of the European Union Council).

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The authors declare that they have no conflict of interest.

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Matias, A.C., Ribeiro, L., Araujo, R.L. et al. Preliminary studies on haematological and plasmatic parameters in gilthead sea bream (Sparus aurata) held under day/night temperature variations. Fish Physiol Biochem 44, 273–282 (2018). https://doi.org/10.1007/s10695-017-0432-6

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