Abstract
Artificial and natural hybridization of dojo loach Misgurnus anguillicaudatus (2N = 50, DD for short) and large-scale loach Paramisgurnus dabryanus (2N = 48, PP for short) are well-grown. However, these hybrid loaches have not yet been examined for fertility and ploidy of gametes. Here, histological observations, artificial propagation, observations of embryonic development, larval morphology, and ploidy analyses were conducted to determine the fertility and ploidy of gametes of allodiploid (DP for short) and allotriploid (DDP for short) loaches, produced by DD females × PP males and induced from fertilized eggs of DD females × PP males by cold shock to prevent the second polar body release, respectively. The ovaries of DP and DDP included smaller number of eggs when compared with those of the control DD, while full-grown oocytes were observed. Testes of these two loaches were delayed-developed without spermatids or mature spermatozoa. Results obtained here showed that DP and DDP were fertility-weakened female and sterile male. Moreover, DP females and DDP females could, respectively, produce few viable haploid eggs and few viable haploid and diploid eggs. This study will provide valuable information for fish hybrid researches.
Similar content being viewed by others
References
Arai K, Mukaino M (1997) Clonal nature of gynogenetically induced progeny of triploid (diploid × tetraploid) loach Misgurnus anguillicaudatus (Pisces: Cobitidiae). J Exp Zool 278:412–420
Arai K, Mukaino M (1998) Electrophoretic analysis of the diploid progenies from triploid × diploid crosses in the loach Misgurnus anguillicaudatus (Pisces: Cobitidae). J Exp Zool 280:368–374
Cao XJ, Wang WM (2009) Histology and mucin histochemistry of the digestive tract of yellow catfish, Pelteobagrus fulvidraco. Anat Histol Embryol 38:254–261. doi:10.1111/j.1439-0264.2009.00932.x
Cherfas NB (1981) Gynogenesis in fish. In: Kirpichnikov VS (ed) Genetic bases of fish selection. Springer-Verlag, Berlin, pp 199–214
Cui L, Fang LB, Yu YY, Wang WM, Zhou XY, Abbas K (2013) Reciprocal hybrids among diploid, tetraploid dojo loach, Misgurnus anguillicaudatus, and large-scale loach, Paramisgurnus dabryanus: fertilization, survival and growth performance. J World Aqua Cult Soc 44:415–424. doi:10.1111/jwas.12035
Fujimoto T, Yasui GS, Yoshikawa H, Yamaha E, Arai K (2008) Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female with M. mizolepis male. J Appl Ichthyo 24:430–437. doi:10.1111/j.1439-0426.2008.01131.x
Gao J, Koshio S, Nguyen BT, Wang WM, Cao XJ (2012) Comparative studies on lipid profiles and amino acid composition of wild and cultured dojo loach Misgurnus anguillicaudatus obtained from southern Japan. Fish Sci 78:1331–1336. doi:10.1007/s12562-012-0561-x
Huang SQ, Cao XJ, Tian XC, Luo WW, Wang WM (2015) Production of tetraploid gynogenetic loach using diploid eggs of natural tetraploid loach, Misgurnus anguillicaudatus, fertilized with UV-irradiated sperm of Megalobrama amblycephala without treatments for chromosome doubling. Cytogenet Genome Res 147:260–267. doi:10.1159/000444384
Li YJ, Zhang MZ, Qian C, Gao M, Arai K (2012) Fertility and ploidy of gametes of diploid, triploid and tetraploid loaches, Misgurnus anguillicaudatus, in China. J Appl Ichthyo 28:900–905
Suzuki R, Nakanishi T, Oshiro T (1985) Survival, growth and sterility of induced triploids in the cyprinid loach Misgurnus anguillicaudatus. Bull Japan Soc Sci Fish 51:889–894
Yasui GS, Arias-Rodriguez L, Fujimoto T, Arai K (2009) A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species. Anim Reprod Sci 116:335–345
You CH, Yu X, Tong JG (2009) Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations. Enviro Biol Fish 86:65–71
Zhang QQ, Arai K, Yamashita M (1998) Cytogenetic mechanisms for triploid and haploid egg formation in the triploid loach Misgurnus anguillicaudatus. J Exp Zool 281:608–619
Zhang QQ, Arai K (1999) Aberrant meioses and viable aneuploid progeny of induced triploid loach (Misgurnus anguillicaudatus) when crossed to natural tetraploids. Aquaculture 175:63–76
Acknowledgements
This study was financially supported by the Fundamental Research Funds for the Central Universities of China (Project Number: 2662015PY033). We specially thank Dr. Roger Edward P. Mamauag for revising this paper.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
This study was conducted in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Huazhong Agricultural University.
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Huang, S., Cao, X., Wang, W. et al. Fertility and ploidy of gametes of allodiploid and allotriploid loaches produced by diploid Misgurnus anguillicaudatus females and Paramisgurnus dabryanus males. Fish Physiol Biochem 44, 13–20 (2018). https://doi.org/10.1007/s10695-017-0409-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10695-017-0409-5