Fish Physiology and Biochemistry

, Volume 43, Issue 6, pp 1761–1773 | Cite as

Effects of overfeeding and high-fat diet on cardiosomatic parameters and cardiac structures in young and adult zebrafish



Obesity is a complex global health problem because it is a risk factor for multiple chronic pathologies such as cardiovascular, endocrine, metabolic, and neoplastic diseases. It is considered a multicausal disease, and one of the determining factors is nutritional imbalances, which include high-fat diets. In this paper, we use the zebrafish model to assess the impact of overfeeding and a high-fat diet in somatic and cardiac parameters in young and adult zebrafish. The results show that fish receiving a high-fat diet showed greater weight gain compared to fish receiving a standard fat diet. Additionally, changes in the heart, including increases in size, a change in the triangular shape of the ventricle to a globular shape, and an increase in the thickness of the trabeculae of the spongy myocardium were observed. These changes could be indicators of cardiovascular overload. The results show that there is a direct relationship between the intake of a high-fat diet and obesity, which in turn can induce cardiac changes, supporting the hypothesis of the relationship between high-fat diets and cardiovascular risk factors. Given the genetic similarity between zebrafish and humans, these results could be extrapolated to human beings, and the findings similarly highlight the importance of incorporating a balanced diet from the early life stages to reduce the risk of cardiovascular disease.


Nutrition Body mass index Obesity Myocardium Cardiovascular risk 



This work was supported by Research Grant from the Pontificia Universidad Javeriana, Bogotá, Colombia. Project: 5605, budget code 120112Z0401200.

Compliance with ethical standards

Ethical statement

No ethical issues are involved in this work.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Bakkers J (2011) Zebrafish as a model to study cardiac development and human cardiac disease. Cardiovasc Res 91(2):279–288. doi: 10.1093/cvr/cvr098 CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bender R, Zeeb H, Schwarz M, Jöckel K-H, Berger M (2006) Causes of death in obesity: relevant increase in cardiovascular but not in all-cancer mortality. J Clin Epidemiol 59(10):1064–1071. doi: 10.1016/j.jclinepi.2006.01.006 CrossRefPubMedGoogle Scholar
  3. Bhatheja S, Panchal HB, Ventura H, Paul TK (2016) Obesity cardiomyopathy: pathophysiologic factors and Nosologic reevaluation. Am J Med Sci 352(2):219–222. doi: 10.1016/j.amjms.2016.05.014 CrossRefPubMedGoogle Scholar
  4. Boney CM, Verma A, Tucker R, Vohr BR (2005) Metabolic syndrome in childhood: association with birth weight, maternal obesity, and gestational diabetes mellitus. Pediatrics 115(3):e290–e296. doi: 10.1542/peds.2004-1808 CrossRefPubMedGoogle Scholar
  5. Boozer CN, Schoenbach G, Atkinson RL (1995) Dietary fat and adiposity: a dose-response relationship in adult male rats fed isocalorically. Am J Physiol Endocrinol Metab 268(4):E546–E550Google Scholar
  6. Bourgeois F, Alexiu A, Lemonnier D (1983) Dietary-induced obesity: effect of dietary fats on adipose tissue cellularity in mice. Br J Nutr 49(1):17–26CrossRefPubMedGoogle Scholar
  7. Bray GA, Paeratakul S, Popkin BM (2004) Dietary fat and obesity: a review of animal, clinical and epidemiological studies. Physiol Behav 83(4):549–555. doi: 10.1016/j.physbeh.2004.08.039 CrossRefPubMedGoogle Scholar
  8. Cali AMG, Caprio S (2008) Obesity in children and adolescents. J Clin Endocrinol Metab 93(11 Suppl 1):S31–S36. doi: 10.1210/jc.2008-1363 CrossRefPubMedPubMedCentralGoogle Scholar
  9. Dietz WH (1998) Health consequences of obesity in youth: childhood predictors of adult disease. Pediatrics 101(Supplement 2):518–525PubMedGoogle Scholar
  10. Ebong IA, Goff DC, Rodriguez CJ, Chen H, Bertoni AG (2014) Mechanisms of heart failure in obesity. Obes Res Clin Pract 8(6):e540–e548. doi: 10.1016/j.orcp.2013.12.005 CrossRefPubMedPubMedCentralGoogle Scholar
  11. de Ferranti S, Mozaffarian D (2008) The perfect storm: obesity, adipocyte dysfunction, and metabolic consequences. Clin Chem 54(6):945–955. doi: 10.1373/clinchem.2007.100156 CrossRefPubMedGoogle Scholar
  12. Flint AJ, Rimm EB (2006) Commentary: obesity and cardiovascular disease risk among the young and old—is BMI the wrong benchmark? Int J Epidemiol 35(1):187–189. doi: 10.1093/ije/dyi298 CrossRefPubMedGoogle Scholar
  13. Flynn EJ, Trent CM, Rawls JF (2009) Ontogeny and nutritional control of adipogenesis in zebrafish (Danio rerio). J Lipid Res 50(8):1641–1652. doi: 10.1194/jlr.M800590-JLR200 CrossRefPubMedPubMedCentralGoogle Scholar
  14. Frankel PH (2003) Obesity and cancer. N Engl J Med 349(5):502–504CrossRefPubMedGoogle Scholar
  15. Galtier-Dereure F, Boegner C, Bringer J (2000) Obesity and pregnancy: complications and cost. Am J Clin Nutr 71(5):1242s–1248sPubMedGoogle Scholar
  16. Gonzales JM, Law SHW (2013) Feed and feeding regime affect growth rate and gonadosomatic index of adult zebrafish (Danio rerio). Zebrafish 10(4):532–540. doi: 10.1089/zeb.2013.0891 CrossRefPubMedPubMedCentralGoogle Scholar
  17. Goodwin N, Westall L, Karp NA, Hazlehurst D, Kovacs C, Keeble R, Bussell J (2016) Evaluating and optimizing fish health and welfare during experimental procedures. Zebrafish 13(S1):S–127. doi: 10.1089/zeb.2015.1165 Google Scholar
  18. Guh DP, Zhang W, Bansback N, Amarsi Z, Birmingham CL, Anis AH (2009) The incidence of co-morbidities related to obesity and overweight: a systematic review and meta-analysis. BMC Public Health 9:88. doi: 10.1186/1471-2458-9-88 CrossRefPubMedPubMedCentralGoogle Scholar
  19. Haag M, Dippenaar NG (2005) Dietary fats, fatty acids and insulin resistance: short review of a multifaceted connection. Med Sci Monit: Int Med J Exp Clin Res 11(12):RA359–RA367Google Scholar
  20. Hussain A, Claussen B, Ramachandran A, Williams R (2007) Prevention of type 2 diabetes: a review. Diabetes Res Clin Pract 76(3):317–326. doi: 10.1016/j.diabres.2006.09.020 CrossRefPubMedGoogle Scholar
  21. Imrie D, Sadler KC (2010) White adipose tissue development in zebrafish is regulated by both developmental time and fish size. Dev Dyn 239(11):3013–3023. doi: 10.1002/dvdy.22443 CrossRefPubMedPubMedCentralGoogle Scholar
  22. Joe AWB, Yi L, Even Y, Vogl AW, Rossi FMV (2009) Depot-specific differences in adipogenic progenitor abundance and proliferative response to high-fat diet. Stem Cells 27(10):2563–2570. doi: 10.1002/stem.190 CrossRefPubMedGoogle Scholar
  23. Kaur H, Hyder ML, Poston WSC (2003) Childhood overweight: an expanding problem. Treat Endocrinol 2(6):375–388CrossRefPubMedGoogle Scholar
  24. Kowalska A, Zakęś Z, Siwicki AK, Jankowska B, Jarmołowicz S, Demska-Zakęś K (2012) Impact of diets with different proportions of linseed and sunflower oils on the growth, liver histology, immunological and chemical blood parameters, and proximate composition of pikeperch Sander lucioperca (L.) Fish Physiol Biochem 38(2):375–388. doi: 10.1007/s10695-011-9514-z CrossRefPubMedGoogle Scholar
  25. Lawrence C (2007) The husbandry of zebrafish (Danio rerio): a review. Aquaculture 269(1–4):1–20. doi: 10.1016/j.aquaculture.2007.04.077 CrossRefGoogle Scholar
  26. Leibold S, Hammerschmidt M (2015) Long-term hyperphagia and caloric restriction caused by low- or high-density husbandry have differential effects on zebrafish postembryonic development, somatic growth, fat accumulation and reproduction. PLoS One 10(3):e0120776. doi: 10.1371/journal.pone.0120776 CrossRefPubMedPubMedCentralGoogle Scholar
  27. Li M, Maddison LA, Page-McCaw P, Chen W (2014) Overnutrition induces β-cell differentiation through prolonged activation of β-cells in zebrafish larvae. Am J Physiol Endocrinol Metab 306(7):E799–E807. doi: 10.1152/ajpendo.00686.2013 CrossRefPubMedPubMedCentralGoogle Scholar
  28. Lichtenstein AH, Schwab US (2000) Relationship of dietary fat to glucose metabolism. Atherosclerosis 150(2):227–243CrossRefPubMedGoogle Scholar
  29. Little TJ, Russo A, Meyer JH, Horowitz M, Smyth DR, Bellon M et al (2007) Free fatty acids have more potent effects on gastric emptying, gut hormones, and appetite than triacylglycerides. Gastroenterology 133(4):1124–1131. doi: 10.1053/j.gastro.2007.06.060 CrossRefPubMedGoogle Scholar
  30. Lobstein T, Baur L, Uauy R (2004) Obesity in children and young people: a crisis in public health. Obes Rev 5:4–85. doi: 10.1111/j.1467-789X.2004.00133.x CrossRefPubMedGoogle Scholar
  31. Mancuso P (2016) The role of adipokines in chronic inflammation. ImmunoTargets Ther 5:47–56. doi: 10.2147/ITT.S73223 CrossRefPubMedPubMedCentralGoogle Scholar
  32. Meguro S, Hasumura T, Hase T (2015) Body fat accumulation in zebrafish is induced by a diet rich in fat and reduced by supplementation with green tea extract. PLoS One 10(3):e0120142. doi: 10.1371/journal.pone.0120142 CrossRefPubMedPubMedCentralGoogle Scholar
  33. Nakae J, Kido Y, Accili D (2001) Distinct and overlapping functions of insulin and IGF-I receptors. Endocr Rev 22(6):818–835. doi: 10.1210/edrv.22.6.0452 CrossRefPubMedGoogle Scholar
  34. Nascimento CMO d, Ribeiro EB, Oyama LM (2009) Metabolism and secretory function of white adipose tissue: effect of dietary fat. An Acad Bras Cienc 81(3):453–466. doi: 10.1590/S0001-37652009000300010 CrossRefGoogle Scholar
  35. Nekoubin H, Rakhshanipour G, Hatefi S, Sudagar M, Montajami S (2013) Effects of feeding frequency on growth performance and survival rate of zebra fish (Danio rerio). Adv J Agric Res ResearchGate 1:7–10Google Scholar
  36. Oka T, Nishimura Y, Zang L, Hirano M, Shimada Y, Wang Z, … Tanaka T (2010) Diet-induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. BMC Physiol 10:21. doi: 10.1186/1472-6793-10-21
  37. Pannevis MC, Earle KE (1994) Maintenance energy requirement of five popular species of ornamental fish. J Nutr 124(12 Suppl):2616S–2618SPubMedGoogle Scholar
  38. Pombo A, Blasco M, Climent V (2012) The status of farmed fish hearts: an alert to improve health and production in three Mediterranean species. Rev Fish Biol Fish 22(3):779–789. doi: 10.1007/s11160-012-9259-5 CrossRefGoogle Scholar
  39. Poppitt SD, Prentice AM (1996) Energy density and its role in the control of food intake: evidence from metabolic and community studies. Appetite 26(2):153–174. doi: 10.1006/appe.1996.0013 CrossRefPubMedGoogle Scholar
  40. Riccardi G, Giacco R, Rivellese AA (2004) Dietary fat, insulin sensitivity and the metabolic syndrome. Clin Nutr 23(4):447–456. doi: 10.1016/j.clnu.2004.02.006 CrossRefPubMedGoogle Scholar
  41. Risnes KR, Vatten LJ, Baker JL, Jameson K, Sovio U, Kajantie E, … Bracken MB (2011). Birthweight and mortality in adulthood: a systematic review and meta-analysis. Int J Epidemiol 40(3):647–661. doi: 10.1093/ije/dyq267
  42. Rössner S (1998) Childhood obesity and adulthood consequences. Acta Paediatr 87(1):1–5. doi: 10.1111/j.1651-2227.1998.tb01375.x CrossRefPubMedGoogle Scholar
  43. Singleman C, Holtzman NG (2011). Heart dissection in larval, juvenile and adult zebrafish, Danio rerio. J Vis Exp (55). doi: 10.3791/3165
  44. Stuart B, Panico L (2016) Early-childhood BMI trajectories: evidence from a prospective, nationally representative British cohort study. Nutr Diabetes 6:e198. doi: 10.1038/nutd.2016.6 CrossRefPubMedPubMedCentralGoogle Scholar
  45. Tingaud-Sequeira A, Ouadah N, Babin PJ (2011) Zebrafish obesogenic test: a tool for screening molecules that target adiposity. J Lipid Res 52(9):1765–1772. doi: 10.1194/jlr.D017012 CrossRefPubMedPubMedCentralGoogle Scholar
  46. Vargas R, Vásquez IC (2016) Cardiac and somatic parameters in zebrafish: tools for the evaluation of cardiovascular function. Fish Physiol Biochem 42(2):569–577. doi: 10.1007/s10695-015-0160-8 CrossRefPubMedGoogle Scholar
  47. Velasco-Santamaría Y, Corredor-Santamaría W (2011) Nutritional requirements of freshwater ornamental fish: a review. Rev MVZ Córdoba 16(2):2458–2469CrossRefGoogle Scholar
  48. Visscher TL, Seidell JC (2001) The public health impact of obesity. Annu Rev Public Health 22(1):355–375. doi: 10.1146/annurev.publhealth.22.1.355 CrossRefPubMedGoogle Scholar
  49. Weiss R, Dziura J, Burgert TS, Tamborlane WV, Taksali SE, Yeckel CW, … Caprio S (2004) Obesity and the metabolic syndrome in children and adolescents. N Engl J Med 350(23):2362–2374. doi: 10.1056/NEJMoa031049
  50. West DB, York B (1998) Dietary fat, genetic predisposition, and obesity: lessons from animal models. Am J Clin Nutr 67(3):505S–512SPubMedGoogle Scholar
  51. Xu, Xue (2016) Pediatric obesity: causes, symptoms, prevention and treatment (review). Exp Ther Med 11(1):15–20. doi: 10.3892/etm.2015.2853 CrossRefPubMedGoogle Scholar
  52. Yiannikouris F, Gupte M, Putnam K, Cassis L (2010) Adipokines and blood pressure control. Curr Opin Nephrol Hypertens 19(2):195–200. doi: 10.1097/MNH.0b013e3283366cd0 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Departamento de Ciencias Fisiológicas, Facultad de MedicinaPontificia Universidad JaverianaBogotáColombia

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