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Modulatory effect of lycopene against carbofuran toxicity in African catfish, Clarias gariepinus

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Abstract

In the current study, African catfish, Clarias gariepinus, was exposed to a sublethal concentration of carbofuran (CF) to investigate its negative effects on biochemical and oxidative stress biomarkers. Also, the putative role of lycopene (LYC) administration in alleviating these negative effects was evaluated. Fish were divided into six groups in triplicates as follows: group I was without treatment, group II was orally administered corn oil, group III was orally administered 18 mg LYC/kg body weight, group IV was exposed to 0.121 mg CF/L, group V was orally administered 9 mg LYC/kg body weight and exposed to 0.121 mg CF/L, and group VI was orally administered 18 mg LYC/kg body weight and exposed to 0.121 mg CF/L for 4 weeks. At the end of this period, blood and tissue (liver and kidney) samples were collected and biochemical and oxidative stress biomarkers were analysed. Also, histopathological changes were determined. Carbofuran caused significant increments of glucose, cortisol, aspartic amino transferase, alanine amino transferase, cholesterol, urea, and creatinine; meanwhile, serum acetylcholinesterase, total protein, albumin, and total lipids were significantly reduced. Significant increments in hepatic and renal malondialdehyde (MDA) and superoxide dismutase (SOD) levels and marked reduction in hepatic and renal catalase (CAT), glutathione (GSH), and total antioxidant capacity (TAC) levels were observed in CF-exposed fish comparing to the control group. Treatment with LYC attenuated the CF-induced oxidative stress, and this improvement was more pronounced in fish received the high LYC dose (18 mg/kg body weight). Further, congestion of the central vein with infiltration of mononuclear inflammatory cells, vacuolar necrosis, and haemorrhage was observed in the livers of CF-exposed fish. Oral administration of LYC reduced behavioural changes and histopathological alterations. All the altered biochemical parameters and antioxidant biomarkers were also restored to be near the normal levels. The obtained results evoked that LYC administration alleviated the destructive effects of carbofuran and reduced its toxicity effect on African catfish.

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Acknowledgements

Funding sources, experimental design, and fish handling of the current study had been approved by the Research Ethical Committee of the Faculty of Women for Arts, Science & Education, Ain Shams University, Cairo, Egypt. Technical assistance for histopathology had been provided by the Central Laboratory, Faculty of Women for Arts, Science & Education, Ain Shams University, Cairo, Egypt.

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Correspondence to Heba S. Hamed.

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Hamed, H.S., Osman, A.G. Modulatory effect of lycopene against carbofuran toxicity in African catfish, Clarias gariepinus . Fish Physiol Biochem 43, 1721–1731 (2017). https://doi.org/10.1007/s10695-017-0404-x

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Keywords

  • Carbofuran
  • Lycopene
  • Clarias gariepinus
  • Biochemistry
  • Oxidative stress biomarkers
  • Histopathology