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Fish Physiology and Biochemistry

, Volume 43, Issue 6, pp 1657–1675 | Cite as

Supplementation of enzyme-treated soy protein saves dietary protein and promotes digestive and absorptive ability referring to TOR signaling in juvenile fish

  • Weiwei Xiao
  • Weidan Jiang
  • Lin Feng
  • Yang Liu
  • Pei Wu
  • Jun Jiang
  • Yongan Zhang
  • Xiaoqiu Zhou
Article
  • 139 Downloads

Abstract

This study was conducted to evaluate the effect of enzyme-treated soy protein (ETSP) supplementation in the low-protein diet on growth performance, digestive and absorptive capacities, and related signaling molecules’ gene expressions in juvenile Jian carp. The results showed that percent weight gain (PWG), specific growth rate (SGR), and feed intake (FI) were decreased by reducing dietary protein from 34 to 32% (P < 0.05). Supplying low-protein diet with optimal ETSP increased previously mentioned indices of juvenile Jian carp (P < 0.05), which also had no significant difference with the high-protein diet (34%CP) (P > 0.05). Compared with the low-protein diet, appropriate ETSP supplementation in the low-protein diet increased (P < 0.05) (1) the trypsin, lipase, and amylase activities in the hepatopancreas; (2) cholecystokinin concentration in the proximal intestine; (3) the γ-glutamyl transpeptidase (γ-GT), alkaline phosphatase (AKP), and Na+/K+-ATPase activities in all intestinal segments; and (4) the messenger RNA (mRNA) levels of trypsin, lipase, and amylase in hepatopancreas and γ-GT in the mid (MI) and distal (DI) intestine, alkaline phosphatase in MI, and Na+/K+-ATPase and target of rapamycin in all intestinal segments. At the same time, appropriate ETSP supplementation in the low-protein diet downregulated the mRNA levels of AKP in the DI and eIF4E-binding protein 2 in all intestinal segments (P < 0.05). In conclusion, adding 10 g ETSP/kg diet in the low-protein diet can restore the growth performance and digestive and absorptive abilities to the levels in group with 34% dietary protein. Supplementation of optimal ETSP in the low-protein diet enhanced the digestive and absorptive abilities and regulated the signaling molecules related to the TOR signaling pathway.

Keywords

Save protein Digestive and absorptive capacity Enzyme-treated soy protein 

Abbreviations

AKP

Alkaline phosphatase

CK

Creatine kinase

DI

Distal intestine

ETSP

Enzyme-treated soy protein

FBW

Final body weight

FE

Feed efficiency

FI

Feed intake

γ-GT

γ-Glutamyl transpeptidase

HIS

Hepatosomatic index

HPC

Hepatopancreas protein content

HW

Hepatopancreas weight

IBW

Initial body weight

IL

Intestinal length

IPC

Intestinal protein content

ISI

Intestosomatic index

IW

Intestinal weight

MI

Mid intestine

PER

Protein efficiency ratio

PI

Proximal intestine

PRV

Protein retention value

PWG

Percent weight gain

RGL

Relative gut length

SGR

Specific growth rate

Notes

Acknowledgements

This research was financially supported by the National Basic Research Program of China (973 Program) (2014CB138600), National Natural Science Foundation of China (31572632), National Department Public Benefit Research Foundation (Agriculture) of China (201003020), Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture), Science and Technology Support Program of Sichuan Province of China (2014NZ0003), Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China (2012NC0007; 2013NC0045), the Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China (2015CC0011), Foundation of Sichuan Youth Science and Technology Innovation Research Team (2017TD0002) and Natural Science Foundation for Young Scientists of Sichuan Province (2014JQ0007). The authors would like to thank the personnel of these teams for their kind assistance.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Weiwei Xiao
    • 1
    • 2
  • Weidan Jiang
    • 1
    • 3
    • 4
  • Lin Feng
    • 1
    • 3
    • 4
  • Yang Liu
    • 1
    • 3
    • 4
  • Pei Wu
    • 1
    • 3
    • 4
  • Jun Jiang
    • 1
    • 3
    • 4
  • Yongan Zhang
    • 5
  • Xiaoqiu Zhou
    • 1
    • 3
    • 4
  1. 1.Animal Nutrition InstituteSichuan Agricultural UniversityChengduChina
  2. 2.Chengdu Mytech Biotech Co., Ltd.ChengduChina
  3. 3.Fish Nutrition and Safety Production University Key Laboratory of Sichuan ProvinceSichuan Agricultural UniversityChengduChina
  4. 4.Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of EducationSichuan Agricultural UniversityChengduChina
  5. 5.Institute of HydrobiologyChinese Academy of SciencesWuhanChina

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