Molecular characterization of the CD79a and CD79b and its role against Aeromonas hydrophila infection in Chinese sucker (Myxocyprinus asiaticus)
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CD79a and CD79b heterodimers are the important signaling components of B cell receptor (BCR) complex which plays a crucial role in B cell development and antibody production. In the present study, CD79a and CD79b homologues from Chinese sucker (Myxocyprinus asiaticus), namely MaCD79a and MaCD79b, were identified and their expression at early developmental stages and under constitutive and stimulated conditions were investigated. The cDNA sequences for MaCD79a and MaCD79b contained open reading frame of 678 and 636 bp in length for 225 and 211 amino acid residues, respectively. The conserved features and important functional residues were found by sequence analysis. RT-PCR analysis revealed that transcripts of MaCD79s were detected in eggs and hatchling at 1–3 and 5–11 days post hatching (dph), but not detected at 13–17 and 19–27 dph, and constantly detected from 30 dph. Tissue distribution analysis showed that MaCD79s was most highly expressed in immune tissues, such as the spleen, head kidney, and kidney; the relatively low levels were detected in the heart, gill, and liver. Results of in situ hybridization also confirmed that MaCD79s is mainly expressed in systematic immune organs. Meanwhile, similar to IgM, MaCD79s-expressing cells in mucosal immune organ including the digestive track and gill were observed. Additionally, significant upregulation of MaCD79s was seen in the head kidney and spleen of Chinese sucker injected with Aeromonas hydrophila by quantitative real-time PCR. Taken together, our findings provided further information regarding fish CD79s gene and its role in adaptive immunity, which will contribute to the preservation and aquaculture of Chinese sucker.
KeywordsMyxocyprinus asiaticus CD79 Expression analysis Aeromonas hydrophila In situ hybridization
We are grateful to Dr. Jing Wei at Southwest University for the suggestion to this article. This work was supported by grant XDJK2012C08 from the Fundamental Research Funds for the Central Universities (Ministry of Education of China) and grant 201203086 from the Special Fund for Agro-scientific Research in the Public Interest of China.
Compliance with ethical standards
All animal experiments were conducted in accordance with the Regulation of Animal Experimentation of Chongqing, China.
- Ballesteros NA, Rodriguez Saint-Jean S, Perez-Prieto SI, Aquilino C, Tafalla C (2014) Modulation of genes related to the recruitment of immune cells in the digestive tract of trout experimentally infected with infectious pancreatic necrosis virus (IPNV) or orally vaccinated. Dev Comp Immunol 44:195–205CrossRefPubMedGoogle Scholar
- Duncan L, Webster K, Gupta V, Nair S, Deane E (2010) Molecular characterisation of the CD79a and CD79b subunits of the B cell receptor complex in the gray short-tailed opossum (Monodelphis domestica) and tammar wallaby (Macropus eugenii): delayed B cell immunocompetence in marsupial neonates. Vet Immunol Immunopathol 136:235–247CrossRefPubMedGoogle Scholar
- Katsukura H, Murakami R, Chijiiwa Y, Otsuka A, Tanaka M, Nakashima K, Ono M (2001) Structure of the beta-chain (B29) gene of the chicken B-cell receptor and conserved collinearity with genes for potential skeletal muscle sodium channel and growth hormone. Immunogenetics 53:770–775CrossRefPubMedGoogle Scholar
- Kraus M, Pao LI, Reichlin A, Hu Y, Canono B, Cambier JC, Nussenzweig MC, Rajewsky K (2001) Interference with immunoglobulin (Ig) alpha immunoreceptor tyrosine-based activation motif (ITAM) phosphorylation modulates or blocks B cell development, depending on the availability of an Ig beta cytoplasmic tail. J Exp Med 194:455–469CrossRefPubMedPubMedCentralGoogle Scholar
- Matsuuchi L, Gold MR, Travis A, Grosschedl R, Defranco AL, Kelly RB (1992) The membrane Igm-associated proteins Mb-1 and Ig-beta are sufficient to promote surface expression of a partially functional B-cell antigen receptor in a nonlymphoid cell-line. Proc Natl Acad Sci U S A 89:3404–3408CrossRefPubMedPubMedCentralGoogle Scholar
- Sahoo PK, Das Mahapatra K, Saha JN, Barat A, Sahoo M, Mohanty BR, Gjerde B, Odegard J, Rye M, Salte R (2008) Family association between immune parameters and resistance to Aeromonas hydrophila infection in the Indian major carp, Labeo rohita. Fish Shellfish Immunol 25:163–169CrossRefPubMedGoogle Scholar
- Salinas I (2015) The mucosal immune system of teleost fish. Biology (Basel) 4:525–539Google Scholar
- Wang S, Xie Y (2004) Zhongguo wu zhong hong se ming lu. Gao deng jiao yu chu ban she, Beijing ShiGoogle Scholar
- Wang YF, Zhang XP, Li H, Wang ZJ (2016) Molecular cloning and expression analysis of a IgM in Myxocyprinus asiaticus. Immunology Journal 03:0192-07.Google Scholar