Fish Physiology and Biochemistry

, Volume 43, Issue 6, pp 1571–1585 | Cite as

Molecular characterization of the CD79a and CD79b and its role against Aeromonas hydrophila infection in Chinese sucker (Myxocyprinus asiaticus)

  • Huan Li
  • Yujin Li
  • Xiaoping Zhang
  • Yafeng Wang
  • Wufeng Zhang
  • Xia Wu
  • Zhijian Wang
Article
  • 127 Downloads

Abstract

CD79a and CD79b heterodimers are the important signaling components of B cell receptor (BCR) complex which plays a crucial role in B cell development and antibody production. In the present study, CD79a and CD79b homologues from Chinese sucker (Myxocyprinus asiaticus), namely MaCD79a and MaCD79b, were identified and their expression at early developmental stages and under constitutive and stimulated conditions were investigated. The cDNA sequences for MaCD79a and MaCD79b contained open reading frame of 678 and 636 bp in length for 225 and 211 amino acid residues, respectively. The conserved features and important functional residues were found by sequence analysis. RT-PCR analysis revealed that transcripts of MaCD79s were detected in eggs and hatchling at 1–3 and 5–11 days post hatching (dph), but not detected at 13–17 and 19–27 dph, and constantly detected from 30 dph. Tissue distribution analysis showed that MaCD79s was most highly expressed in immune tissues, such as the spleen, head kidney, and kidney; the relatively low levels were detected in the heart, gill, and liver. Results of in situ hybridization also confirmed that MaCD79s is mainly expressed in systematic immune organs. Meanwhile, similar to IgM, MaCD79s-expressing cells in mucosal immune organ including the digestive track and gill were observed. Additionally, significant upregulation of MaCD79s was seen in the head kidney and spleen of Chinese sucker injected with Aeromonas hydrophila by quantitative real-time PCR. Taken together, our findings provided further information regarding fish CD79s gene and its role in adaptive immunity, which will contribute to the preservation and aquaculture of Chinese sucker.

Keywords

Myxocyprinus asiaticus CD79 Expression analysis Aeromonas hydrophila In situ hybridization 

Notes

Acknowledgements

We are grateful to Dr. Jing Wei at Southwest University for the suggestion to this article. This work was supported by grant XDJK2012C08 from the Fundamental Research Funds for the Central Universities (Ministry of Education of China) and grant 201203086 from the Special Fund for Agro-scientific Research in the Public Interest of China.

Compliance with ethical standards

All animal experiments were conducted in accordance with the Regulation of Animal Experimentation of Chongqing, China.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Huan Li
    • 1
  • Yujin Li
    • 1
  • Xiaoping Zhang
    • 1
  • Yafeng Wang
    • 1
  • Wufeng Zhang
    • 1
  • Xia Wu
    • 1
  • Zhijian Wang
    • 2
  1. 1.Key Laboratory of Eco-environment in the Three Gorges Reservoir Region of Ministry of Education, School of Life SciencesSouthwest UniversityChongqingChina
  2. 2.Key Laboratory of Freshwater Fish Reproduction Development, Ministry of Education, School of Life SciencesSouthwest UniversityChongqingChina

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