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Effect of N-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, and antioxidative ability of grass carp, Ctenopharyngodon idella

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Abstract

An 8-week feeding trial was conducted to evaluate the effect of N-acetyl cysteine (NAC) and glycine supplementation on growth performance, glutathione (GSH) synthesis, and antioxidative ability of grass carp, Ctenopharyngodon idella. Four practical diets were formulated: control, control + 0.2% NAC, control + 0.5% glycine, and control + 0.2% NAC + 0.5% glycine. Each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 8.8 g). Weight gain and specific growth rate were significantly increased with the supplementation of NAC. Supplementation of NAC plus glycine significantly increased the feed efficiency. Glutathione peroxidase (GPx) and γ-glutamine cysteine synthase (γ-GCS) in plasma were significantly increased with the supplementation of NAC plus glycine. GSH in plasma increased and malondialdehyde (MDA) decreased in fish fed diets supplemented with NAC. Respiratory burst, superoxide dismutase (SOD), and catalase (CAT) activity were not affected by NAC or glycine. These results clearly indicated that NAC improved the growth performance and restored GSH of grass carp, supplemented NAC together with glycine enhanced GSH synthesis, and improved the antioxidative ability of grass carp.

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Acknowledgments

This work was supported by the Project of Marine Fishery Science and Technology Extension of Guangdong Province (A201301C02).

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Authors and Affiliations

  1. Nutrition Laboratory, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China

    Shiwei Xie, Lixia Tian, Jin Niu, Guiying Liang & Yongjian Liu

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  1. Shiwei Xie
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  2. Lixia Tian
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  3. Jin Niu
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  4. Guiying Liang
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  5. Yongjian Liu
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Correspondence to Yongjian Liu.

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Xie, S., Tian, L., Niu, J. et al. Effect of N-acetyl cysteine and glycine supplementation on growth performance, glutathione synthesis, and antioxidative ability of grass carp, Ctenopharyngodon idella . Fish Physiol Biochem 43, 1011–1020 (2017). https://doi.org/10.1007/s10695-017-0348-1

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  • DOI: https://doi.org/10.1007/s10695-017-0348-1

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