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hsp90 and hsp47 appear to play an important role in minnow Puntius sophore for surviving in the hot spring run-off aquatic ecosystem

Abstract

Changes in the expression of a number of hsp genes in minnow Puntius sophore collected from a hot spring run-off (Atri hot spring in Odisha, India; 20o09′N 85°18′E, 36–38 °C) were investigated to study the upper thermal acclimation response under heat stress, using same species from aquaculture ponds (water temperature 27 °C) as control. Expression of hsp genes was analyzed in both groups using RT-qPCR, which showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in hot spring run-off fishes, whereas there was no alteration in expression of other hsps. As the fish inhabit the hot spring run-off area for very long duration, they could have adapted to the environment. To test this hypothesis, fishes collected from hot spring run-off were divided into two groups; one was heat-shocked at 41 °C/24 h, and the other was acclimatized at 27 °C/24 h. Up-regulation of all the hsps (except hsp78) was observed in the heat-shocked fishes, whereas expression of all hsps was found to be down-regulated to the basal level in fishes maintained at 27 °C/24 h. Pathway analysis showed that the expressions of all the hsps except hsp90 are regulated by the transcription factor heat shock factor 1 (Hsf1). This study showed that hsp90 and hsp47 play an important role in Puntius sophore for surviving in the high-temperature environment of the hot spring run-off. Additionally, we show that plasticity in hsp gene expression is not lost in the hot spring run-off population.

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Acknowledgments

This research was funded by the Indian Council of Agricultural Research under the National Fund for Basic, Strategic and Frontier Application Research in Agriculture (NFBSFARA; recently renamed National Agricultural Science Fund, NASF) Project # AS-2001 (B.P.M. and S.M). A.M. and G.K.P. are NFBSFARA Senior Research Fellows. The authors are thankful to Director, ICAR—Central Inland Fisheries Research Institute, Barrackpore, and Director, School of Biotechnology, KIIT University, Bhubaneswar, for the facilities and encouragement. Technical assistance received from Shri Laddu Ram Mahaver, Samir K. Paul and Rabiul Sk. is acknowledged. The authors would like to acknowledge the anonymous reviewers for critically reviewing the manuscript; the constructive criticism and suggestions from the reviewers have resulted in substantial improvement of the manuscript.

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Mahanty, A., Purohit, G.K., Yadav, R.P. et al. hsp90 and hsp47 appear to play an important role in minnow Puntius sophore for surviving in the hot spring run-off aquatic ecosystem. Fish Physiol Biochem 43, 89–102 (2017). https://doi.org/10.1007/s10695-016-0270-y

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Keywords

  • Heat stress
  • Thermal acclimation
  • hsp gene
  • Gene plasticity
  • RT-qPCR