Fish Physiology and Biochemistry

, Volume 43, Issue 1, pp 89–102 | Cite as

hsp90 and hsp47 appear to play an important role in minnow Puntiussophore for surviving in the hot spring run-off aquatic ecosystem

  • Arabinda Mahanty
  • Gopal Krishna Purohit
  • Ravi Prakash Yadav
  • Sasmita Mohanty
  • Bimal Prasanna Mohanty
Article

Abstract

Changes in the expression of a number of hsp genes in minnow Puntiussophore collected from a hot spring run-off (Atri hot spring in Odisha, India; 20o09′N 85°18′E, 36–38 °C) were investigated to study the upper thermal acclimation response under heat stress, using same species from aquaculture ponds (water temperature 27 °C) as control. Expression of hsp genes was analyzed in both groups using RT-qPCR, which showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in hot spring run-off fishes, whereas there was no alteration in expression of other hsps. As the fish inhabit the hot spring run-off area for very long duration, they could have adapted to the environment. To test this hypothesis, fishes collected from hot spring run-off were divided into two groups; one was heat-shocked at 41 °C/24 h, and the other was acclimatized at 27 °C/24 h. Up-regulation of all the hsps (except hsp78) was observed in the heat-shocked fishes, whereas expression of all hsps was found to be down-regulated to the basal level in fishes maintained at 27 °C/24 h. Pathway analysis showed that the expressions of all the hsps except hsp90 are regulated by the transcription factor heat shock factor 1 (Hsf1). This study showed that hsp90 and hsp47 play an important role in Puntiussophore for surviving in the high-temperature environment of the hot spring run-off. Additionally, we show that plasticity in hsp gene expression is not lost in the hot spring run-off population.

Keywords

Heat stress Thermal acclimation hsp gene Gene plasticity RT-qPCR 

Supplementary material

10695_2016_270_MOESM1_ESM.doc (58 kb)
Supplementary material 1 (DOC 58 kb)
10695_2016_270_MOESM2_ESM.doc (47 kb)
Supplementary material 2 (DOC 47 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Arabinda Mahanty
    • 1
    • 2
  • Gopal Krishna Purohit
    • 2
  • Ravi Prakash Yadav
    • 2
  • Sasmita Mohanty
    • 2
  • Bimal Prasanna Mohanty
    • 1
  1. 1.Biochemistry Laboratory, Proteomics Unit, Fishery Resource and Environmental Management DivisionICAR- Central Inland Fisheries Research InstituteBarrackpore, KolkataIndia
  2. 2.KIIT School of BiotechnologyKIIT UniversityBhubaneswarIndia

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