hsp90 and hsp47 appear to play an important role in minnow Puntius sophore for surviving in the hot spring run-off aquatic ecosystem
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Changes in the expression of a number of hsp genes in minnow Puntius sophore collected from a hot spring run-off (Atri hot spring in Odisha, India; 20o09′N 85°18′E, 36–38 °C) were investigated to study the upper thermal acclimation response under heat stress, using same species from aquaculture ponds (water temperature 27 °C) as control. Expression of hsp genes was analyzed in both groups using RT-qPCR, which showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in hot spring run-off fishes, whereas there was no alteration in expression of other hsps. As the fish inhabit the hot spring run-off area for very long duration, they could have adapted to the environment. To test this hypothesis, fishes collected from hot spring run-off were divided into two groups; one was heat-shocked at 41 °C/24 h, and the other was acclimatized at 27 °C/24 h. Up-regulation of all the hsps (except hsp78) was observed in the heat-shocked fishes, whereas expression of all hsps was found to be down-regulated to the basal level in fishes maintained at 27 °C/24 h. Pathway analysis showed that the expressions of all the hsps except hsp90 are regulated by the transcription factor heat shock factor 1 (Hsf1). This study showed that hsp90 and hsp47 play an important role in Puntius sophore for surviving in the high-temperature environment of the hot spring run-off. Additionally, we show that plasticity in hsp gene expression is not lost in the hot spring run-off population.
KeywordsHeat stress Thermal acclimation hsp gene Gene plasticity RT-qPCR
This research was funded by the Indian Council of Agricultural Research under the National Fund for Basic, Strategic and Frontier Application Research in Agriculture (NFBSFARA; recently renamed National Agricultural Science Fund, NASF) Project # AS-2001 (B.P.M. and S.M). A.M. and G.K.P. are NFBSFARA Senior Research Fellows. The authors are thankful to Director, ICAR—Central Inland Fisheries Research Institute, Barrackpore, and Director, School of Biotechnology, KIIT University, Bhubaneswar, for the facilities and encouragement. Technical assistance received from Shri Laddu Ram Mahaver, Samir K. Paul and Rabiul Sk. is acknowledged. The authors would like to acknowledge the anonymous reviewers for critically reviewing the manuscript; the constructive criticism and suggestions from the reviewers have resulted in substantial improvement of the manuscript.
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Conflict of interest
The authors declare that they have no conflicts of interests.
- Boyd CE (1998) Water quality for pond aquaculture. International Center for Aquaculture and Aquatic Environment, Auburn University, Auburn, Albania http://www.extension.org/mediawiki/files/6/61/Water_Quality_for_Pond_Aquaculture.pdf
- CPCB (2007) Guidelines for water quality monitoring. Central Pollution Control Board, New DelhiGoogle Scholar
- Dahanukar N (2010) Puntius sophore. The IUCN Red List of Threatened Species 2010: e.T166623A6249514. http://dx.doi.org/10.2305/IUCN.UK.2010-4.RLTS.T166623A6249514.en
- Das A, Panda SS, Palita SK, Patra HK, Dhal NK (2012) Spatial and temporal variation of phytoplanktons in hot spring of Atri, Odisha, India. Curr Bot 3(5):35–40Google Scholar
- http://www.pathwaycommons.org/about/. Accessed 15 Jan 2016
- Leach MD, Budge S, Walker L, Munro C, Cowen LE, Brown AJP (2012) Hsp90 orchestrates transcriptional regulation by Hsf1 and cell wall remodelling by MAPK signalling during thermal adaptation in a pathogenic yeast. PLoS ONE 8(12):e1003069Google Scholar
- Martínez-Paz P, Morales M, Martín R, Martínez-Guitarte JL, Morcillo G (2014) Characterization of the small heat shock protein Hsp27 gene in Chironomus riparius (Diptera) and its expression profile in response to temperature changes and xenobiotic exposures. Cell Stress Chaperones 19:529–540CrossRefPubMedGoogle Scholar
- McDiarmid RW, Altig R (1999) Tadpoles: the biology of anuran larvae. University of Chicago Press, Chicago, p 202Google Scholar
- Mohanty S, Mahanty A, Yadav RP, Purohit GK, Mohanty BN, Mohanty BP (2014) The Atri hot spring in Odisha—a natural ecosystem for global warming research. Int J Geol Earth Environ Sci 4:85–90Google Scholar
- Mohanty BP, Mitra T, Banerjee S, Bhattacharjee S, Mahanty A, Ganguly S, Purohit GK, Karunakaran D, Mohanty S (2015) Proteomic profiling of white muscle from freshwater catfish Rita rita. Physiol Biochem 41:789–802Google Scholar
- Reddy DV, Nagbhushanam P, Ramesh G (2013) Turnover time of Tural and Rajvadi hot spring waters, Maharashtra, India. Curr Sci 104(10):1419–1424Google Scholar
- Stone NM, Thomforde HK (2004) Understanding Your Fish Pond Water Analysis Report. Cooperative Extension Program, University of Arkansas at Pine Bluff Aquaculture/FisheriesGoogle Scholar
- Tomanek L, Somero GN (2002) Interspecific- and acclimation-induced variation in levels of heat-shock proteins 70 (hsp70) and 90 (hsp90) and heat-shock transcription factor-1 (HSF1) in congeneric marine snails (genus Tegula): implications for regulation of hsp gene expression. J Exp Biol 205(5):677–685PubMedGoogle Scholar
- Zunino B, Rubio-Patiño C, Villa E, Meynet O, Proics E, Cornille A, Pommier S, Mondragón L, Chiche J, Bereder J-M, Carles M, Ricci J-E (2016) Hyperthermic intraperitoneal chemotherapy leads to an anticancer immune response via exposure of cell surface heat shock protein 90. Oncogene 35:261–268CrossRefPubMedGoogle Scholar