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Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer

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Abstract

The Na+/K+-ATPase (NKA) is considered as the main pump involved in active ion transport. In the European sea bass, Dicentrarchus labrax, we found two genes encoding for the alpha 1 subunit isoforms (NKA α1a and NKA α1b). NKA α1a and NKA α1b isoform amino acid (aa) sequences were compared through phylogeny and regarding key functional motifs between salmonids and other acanthomorph species. Analysis of aa sequences of both isoforms revealed a high degree of conservation across teleosts. The expression pattern of both nka α1a and nka α1b was measured in the gill, kidney and posterior intestine of fish in seawater (SW) and transferred to fresh water (FW) at different exposure times. Nka α1a was more expressed than nka α1b whatever the condition and the tissue analyzed. After long-term salinity acclimation (2.5 years) either in FW or SW, transcript levels of nka α1a were higher in the kidney followed by the posterior intestine and the gill. Compared to SW conditions, expression of nka α1a in FW was significantly increased or decreased, respectively, in gill and posterior intestine. In contrast, branchial nka α1b was significantly decreased in FW-acclimated fish. Short-term FW acclimation seems to rapidly increase nka α1a transcript levels in the kidney unlike in gill tissues where different gene expression levels are detected only after long-term acclimation.

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Acknowledgments

The authors thank E. Desmarais UMR 5554, Institut des Sciences de l’Evolution de Montpellier (ISEM) for giving us access to the European sea bass D. labrax genome database and for his help in the sequence analyses. The authors also would like to thank V. Boulo for initiating the nka gene sequence research in the European sea bass.

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  1. Adaptation Ecophysiologique et Ontogenèse (AEO, UMR 9190 MARBEC), Université de Montpellier, Place Eugène Bataillon – CC092, 34095, Montpellier Cedex 5, France

    Eva Blondeau-Bidet, Maryline Bossus, Emilie Farcy, Jehan-Hervé Lignot & Catherine Lorin-Nebel

  2. Unité Biologie des Organismes et écosystèmes aquatiques (BOREA, UMR 7208), CNRS, IRD 207, Sorbonne Universités, Muséum national d’Histoire naturelle, Université Pierre et Marie Curie, Université de Caen Basse Normandie, CP32, 7 rue Cuvier, 75005, Paris, France

    Gersende Maugars

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  1. Eva Blondeau-Bidet
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  2. Maryline Bossus
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  3. Gersende Maugars
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  4. Emilie Farcy
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Correspondence to Catherine Lorin-Nebel.

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Table S1

Supplementary amino acid sequences used in phylogenetic analyses (DOCX 23 kb)

Table S2

Predicted phosphorylation sites in the different analyzed species according to GPS3, compared to previously referenced phosphorylation sites in rat (ref. P06685) using Uniprot database (DOCX 16 kb)

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Blondeau-Bidet, E., Bossus, M., Maugars, G. et al. Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer. Fish Physiol Biochem 42, 1647–1664 (2016). https://doi.org/10.1007/s10695-016-0247-x

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