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Echium oil increased the expression of a Δ4 Fads2 fatty acyl desaturase and the deposition of n-3 long-chain polyunsaturated fatty acid in comparison with linseed oil in striped snakehead (Channa striata) muscle

Fish Physiology and Biochemistry volume 42, pages 1107–1122 (2016)Cite this article

Abstract

Despite the potential of vegetable oils as aquafeed ingredients, a major drawback associated with their utilization is the inferior level of beneficial n-3 long-chain polyunsaturated fatty acids (LC-PUFA). Echium oil (EO), which is rich in stearidonic acid (SDA, 18:4n-3), could potentially improve the deposition of n-3 LC-PUFA as the biosynthesis of LC-PUFA is enhanced through bypassing the rate-limiting ∆6 desaturation step. We report for the first time an attempt to investigate whether the presence of a desaturase (Fads2) capable of ∆4 desaturation activities and an elongase (Elovl5) will leverage the provision of dietary SDA to produce a higher rate of LC-PUFA bioconversion. Experimental diets were designed containing fish oil (FO), EO or linseed oil (LO) (100FO, 100EO, 100LO), and diets which comprised equal mixtures of the designated oils (50EOFO and 50EOLO) were evaluated in a 12-week feeding trial involving striped snakeheads (Channa striata). There was no significant difference in growth and feed conversion efficiency. The hepatic fatty acid composition and higher expression of fads2 and elovl5 genes in fish fed EO-based diets indicate the utilization of dietary SDA for LC-PUFA biosynthesis. Collectively, this resulted in a higher deposition of muscle eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) compared to LO-based diets. Dietary EO improved the ratio of n-3 LC-PUFA to n-6 LC-PUFA in fish muscle, which is desirable for human populations with excessive consumption of n-6 PUFA. This study validates the contribution of SDA in improving the content of n-3 LC-PUFA and the ratio of EPA to arachidonic acid (ARA, 20:4n-6) in a freshwater carnivorous species.

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Acknowledgments

This research was funded by the Universiti Sains Malaysia (RU 1001/PBIOLOGI/855003) and the EScience (Ministry of Agriculture and Agro-based Industry 305/PBIOLOGI/613523). The authors wish to thank Technology Crops Ltd. for providing the echium oil sample used in this study.

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  1. Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Annette Jaya-Ram & Alexander Chong Shu-Chien

  2. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Alexander Chong Shu-Chien & Meng-Kiat Kuah

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  1. Annette Jaya-Ram
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  2. Alexander Chong Shu-Chien
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  3. Meng-Kiat Kuah
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Correspondence to Alexander Chong Shu-Chien.

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Jaya-Ram, A., Shu-Chien, A.C. & Kuah, MK. Echium oil increased the expression of a Δ4 Fads2 fatty acyl desaturase and the deposition of n-3 long-chain polyunsaturated fatty acid in comparison with linseed oil in striped snakehead (Channa striata) muscle. Fish Physiol Biochem 42, 1107–1122 (2016). https://doi.org/10.1007/s10695-016-0201-y

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Keywords

  • Channa striata
  • ∆4 Desaturase
  • Stearidonic acid
  • LC-PUFA
  • Nutritional regulation
  • Echium oil