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Insights into Trx1, TRP14, and Prx1 homologs of Paralichthys olivaceus: molecular profiles and transcriptional responses to immune stimulations

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Abstract

Thioredoxin (Trx) proteins are involved in several cellular processes, such as anti-oxidative stress and cellular redox homeostasis. In this study, we isolated the full-length cDNAs of PoTrx1 and PoTRP14 from Japanese flounder (Paralichthys olivaceus). PoTrx1 is 723 bp in length, with a 366-bp open reading frame (ORF) that encodes for 121 amino acids. PoTRP14 is 909 bp in length, with a 372-bp ORF that encodes for 123 amino acids. PoTrx1 and PoTRP14 are highly conserved in Cys–Gly–Pro–Cys and Cys–Pro–Asp–Cys forms, respectively. Tissue distribution analysis revealed that the transcripts of PoTrx1 and PoTRP14 were ubiquitously expressed in all tested tissues and particularly abundant in immunity-related organs, such as the liver, intestine, gill, and spleen. Development expression profiles indicated that PoTrx1 transcript was expressed from the neurula stage to the 1 day post-hatching stage; the maximum transcript levels were recorded at the somatic stage. The mRNA level of PoTRP14 was constantly expressed at all examined developmental stages, reaching the peak at the before-hatching stage. Prx1 is a peroxiredoxin family member that serves similar functions to PoTrx1 and PoTRP14. A primary hepatocyte culture system was established to examine the immunoregulatory properties of PoTrx1, PoTRP14, and Prx1 in response to lipopolysaccharide, CuSO4, and H2O2 stimulation. Results revealed that the transcript levels of PoTrx1, PoTRP14, and Prx1 were significantly up-regulated in a time-dependent manner after the immunostimulant challenge. These data suggest that PoTrx1, PoTRP14, and Prx1 play critical roles in anti-oxidation and immunoregulation.

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Acknowledgments

This work was supported by the National High-Tech Research and Development Program (2012AA10A402) and the National Science Foundation of China (31172385).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Junqing Yuan, Jiajun Jiang, Liming Jiang, Fang Yang, Yan Chen, Yan He & Quanqi Zhang

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  1. Junqing Yuan
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  2. Jiajun Jiang
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  3. Liming Jiang
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Correspondence to Yan He.

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Yuan, J., Jiang, J., Jiang, L. et al. Insights into Trx1, TRP14, and Prx1 homologs of Paralichthys olivaceus: molecular profiles and transcriptional responses to immune stimulations. Fish Physiol Biochem 42, 547–561 (2016). https://doi.org/10.1007/s10695-015-0158-2

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