Fish Physiology and Biochemistry

, Volume 41, Issue 6, pp 1403–1417 | Cite as

Proteomic analysis of early-stage embryos: implications for egg quality in hapuku (Polyprion oxygeneios)

  • Yair Y. Kohn
  • Jane E. Symonds
  • Torsten Kleffmann
  • Shinichi Nakagawa
  • Malgorzata Lagisz
  • P. Mark Lokman


In order to develop biomarkers that may help predict the egg quality of captive hapuku (Polyprion oxygeneios) and provide potential avenues for its manipulation, the present study (1) sequenced the proteome of early-stage embryos using isobaric tag for relative and absolute quantification analysis, and (2) aimed to establish the predictive value of the abundance of identified proteins with regard to egg quality through regression analysis. Egg quality was determined for eight different egg batches by blastomere symmetry scores. In total, 121 proteins were identified and assigned to one of nine major groups according to their function/pathway. A mixed-effects model analysis revealed a decrease in relative protein abundance that correlated with (decreasing) egg quality in one major group (heat-shock proteins). No differences were found in the other protein groups. Linear regression analysis, performed for each identified protein separately, revealed seven proteins that showed a significant decrease in relative abundance with reduced blastomere symmetry: two correlates that have been named in other studies (vitellogenin, heat-shock protein-70) and a further five new candidate proteins (78 kDa glucose-regulated protein, elongation factor-2, GTP-binding nuclear protein Ran, iduronate 2-sulfatase and 6-phosphogluconate dehydrogenase). Notwithstanding issues associated with multiple statistical testing, we conclude that these proteins, and especially iduronate 2-sulfatase and the generic heat-shock protein group, could serve as biomarkers of egg quality in hapuku.


Hapuku Polyprion oxygeneios Egg quality Proteomics iTRAQ Biomarkers 



We thank the technical staff at the Centre for Protein Research, University of Otago, for their assistance in performing the iTRAQ analysis. We also thank the technical staff at NIWA Aquaculture Park in Bream Bay, Ruakaka, for their assistance with sample collection and Mr Ken Miller, University of Otago, for his assistance with tidying up some of the figures. We thank Dr Alvin Setiawan for providing useful comments to the manuscript draft.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Yair Y. Kohn
    • 1
    • 2
    • 4
  • Jane E. Symonds
    • 2
  • Torsten Kleffmann
    • 3
  • Shinichi Nakagawa
    • 5
  • Malgorzata Lagisz
    • 5
  • P. Mark Lokman
    • 1
  1. 1.Department of ZoologyUniversity of OtagoDunedinNew Zealand
  2. 2.Bream Bay Aquaculture ParkNIWARuakakaNew Zealand
  3. 3.Centre for Protein ResearchUniversity of OtagoDunedinNew Zealand
  4. 4.Arava Research and Development StationHatzevaIsrael
  5. 5.Evolution & Ecology Research Centre and School of Biological, Earth and Environmental SciencesUniversity of New South WalesSydneyAustralia

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