Skip to main content

Advertisement

SpringerLink
Log in

Proteomic profiling of white muscle from freshwater catfish Rita rita

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Muscle tissues contribute 34–48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for many aspects of aquaculture including fish physiology and growth, flesh quality, food safety and aquatic environmental monitoring. The freshwater catfish Rita rita of the family Bagridae inhabiting the tropical rivers and estuaries is an important food fish with high nutritive value and is also considered a species of choice in riverine pollution monitoring. Omics information that could enhance utility of this species in molecular research is meager. Therefore, in the present study, proteomic analysis of Rita rita muscle has been carried out and functional genomics data have been generated. A reference muscle proteome has been developed, and 23 protein spots, representing 18 proteins, have been identified by MALDI-TOF/TOF-MS and LC-MS/MS. Besides, transcript information on a battery of heat shock proteins (Hsps) has been generated. The functional genomics information generated could act as the baseline data for further molecular research on this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Addis MF, Cappuccinelli R, Tedde V, Pagnozzi D, Porcu MC, Bonaglini E, Roggio T, Uzzau S (2010) Proteomic analysis of muscle tissue from gilthead sea bream (Sparus aurata) farmed in offshore floating cages. Aquaculture 309:245–252

    Article  CAS  Google Scholar 

  • Al-Arabi SAM, Goksøyr A (2002) Cytochrome P4501A responses in two tropical fish species, riverine catfish (Rita rita) and marine mudfish (Apocryptes bato). Comp Biochem Physiol Part C Toxicol Pharmacol 131:61–71

    Article  CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Barik SK, Banerjee S, Bhattacharjee S, Dasgupta S, Mohanty S, Mohanty BP (2013) Proteomic analysis of sarcoplasmic peptides of two related fish species for food authentication. Appl Biochem Biotechnol 171:1011–1021

    Article  CAS  PubMed  Google Scholar 

  • Bhattacharjee S, Mohanty S, Sharma AP, Mohanty BP (2011) Effect of storage temperature as a preanalytical variable on the lens crystallins protein quality for proteomic studies. Proteomics Clin Appl 5:504–512

    Article  CAS  PubMed  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Bringans S, Eriksen S, Kendrick T, Gopalakrishnakone P, Livk A, Lock R, Lipscombe R (2008) Proteomic analysis of the venom of Heterometrus longimanus (Asian black scorpion). Proteomics 8:1081–1096

    Article  CAS  PubMed  Google Scholar 

  • Deane EE, Woo NYS (2005) Cloning and characterization of the hsp70 multigene family from silver Sea bream: modulation gene expression between warm and cold temperature acclimatization. Biochem Biophy Res Commun 330(3):776–783

    Article  CAS  Google Scholar 

  • Easton DP, Kaneko Y, Subjeck JR (2000) The Hsp110 and Grp170 stress proteins: newly “recognized relatives of the Hsp70s. Cell Stress Chaperones 5(4):276–290

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Emami K, Askari V, Ullrich M, Mohinudeen K, Anil AC, Khandeparker L, Burgess JG, Mesbahi E (2012) Characterization of bacteria in ballast water using MALDI-TOF/TOF mass spectrometry. PLoS ONE 7:e38515

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Godiksen H, Morzel M, Hyldig G, Jessen F (2009) Contribution of cathepsins B, L and D to muscle protein profiles correlated with texture in rainbow trout (Oncorhynchus mykiss). Food Chem 113:889–896

    Article  CAS  Google Scholar 

  • Hamilton AM, Heikkila JJ (2006) Examination of the stress-induced expression of the collagen binding heat shock protein, hsp47, in Xenopus laevis cultured cells and embryos. Comp Biochem Physiol 143(1):133–141

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Mann M, Hendrickson RC, Pandey A (2001) Analysis of proteins and proteomes by mass spectrometry. Annu Rev Biochem 70:437–473

    Article  CAS  PubMed  Google Scholar 

  • Martin SAM, Mohanty BP, CashP Houlihan DF, Secombes CJ (2007) Proteome analysis of the Atlantic salmon (Salmosalar) cell line SHK-1 following recombinant IFN-γ stimulation. Proteomics 7:2275–2286

    Article  CAS  PubMed  Google Scholar 

  • Mitra T, Ganguly S, Banerjee S, Mahanty A, Mohanty BP (2014) Nutrient profile of freshwater catfish Rita rita (Hamilton) from river Ganga. Book of Abstracts, The 10th Indian Fisheries and Aquaculture Forum (10ifaf) ICAR – National Bureau of Fish Genetic Resources, Lucknow, India 12–15 November 2014

  • Mohanty BP, Bhattacharjee S, Mondal K, Das MK (2010) HSP70 expression profiles in white muscles of riverine catfish Rita rita show promise as biomarker for pollution monitoring in tropical rivers. Natl Acad Sci Lett 33:177–182

    CAS  Google Scholar 

  • Mohanty BP, Bhattacharjee S, Das MK (2011) Lens proteome map and α-crystallin profile of the catfish Rita rita. Ind J Biochem Biophys 48:35–41

    CAS  Google Scholar 

  • Mohanty BP, Banerjee S, Bhattachrjee S, Mitra T, Purohit GK, Sharma AP, Karunakaran D, Mohanty S (2013a) Muscle proteomics of the Indian major carp catla (Catla catla, Hamilton). J Proteomics Bioinform 6:252–263

    Article  CAS  Google Scholar 

  • Mohanty BP, Bhattacharjee S, Paria P, Mahanty A, Sharma AP (2013b) Lipid biomarkers of lens ageing. Appl Biochem Biotechnol 169:192–200

    Article  CAS  PubMed  Google Scholar 

  • Nguyen NH, Ponzoni RW, Hamzah A, Yee HY, Abu-Bakar KR, Khaw HL (2004) Genetics of flesh quality in fish. Fish Aqua 211:65–79

    Google Scholar 

  • Ohlendieck K (2011) Skeletal muscle proteomics: current approaches, technical challenges and emerging techniques. Skelet Muscle 1:6

    Article  PubMed Central  PubMed  Google Scholar 

  • Picard B, Lefèvre F, Lebret B (2012) Meat and fish flesh quality improvement with proteomic applications. Animal Front 2:18–25

    Article  Google Scholar 

  • Porte C, Biosca X, Sole M, Albaiges J (2001) The integrated use of chemical analysis, cytochrome P450 and stress proteins in mussels to assess pollution along the Galician coast (NW Spain). Environ Pollut 112:261–268

    Article  CAS  PubMed  Google Scholar 

  • Purohit GK, Mahanty A, Suar M, Sharma AP, Mohanty BP, Mohanty S (2014) Investigating hsp gene expression in liver of Channa striatus under heat stress for understanding the upper thermal acclimation. Biomed Res Int 2014:10

    Article  Google Scholar 

  • Roberts RJ, Agius C, Saliba C, Bossier P, Sung YY (2010) Heat shock proteins (chaperones) in fish and shellfish and their potential role in relation to fish health: a review. J Fish Dis 33:789–801

    Article  CAS  PubMed  Google Scholar 

  • Terova G, Pisanu S, Roggio T, Preziosa E, Saroglia M, Addis MF (2014) Proteomic profiling of sea bass muscle by two-dimensional gel electrophoresis and tandem mass spectrometry. Fish Physiol Biochem 40(1):311–322

    Article  CAS  PubMed  Google Scholar 

  • Tucker NR, Ustyugov A, Bryantsev AL, Konkel ME, Shelden EA (2009) Hsp27 is persistently expressed in zebrafish skeletaland cardiac muscle tissues but dispensable for their morphogenesis. Cell Stress Chaperones 14:521–533

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Van Aggelen G, Ankley GT, Baldwin WS et al (2010) Integrating omic technologies into aquatic ecological risk assessment and environmental monitoring: hurdles, achievements, and future outlook. Environ Health Perspect 118:1–5

    PubMed Central  PubMed  Google Scholar 

  • Veiseth-Kent E, Grove H, Faergestad EM, Fjaera SO (2010) Changes in muscle and blood plasma proteomes of Atlantic salmon (Salmo salar) induced by crowding. Aquaculture 309:272–279

    Article  CAS  Google Scholar 

  • Williams TL, Andrzejewski D, Lay JO, Musser SM (2003) Experimental factors affecting the quality and reproducibility of MALDI TOF mass spectra obtained from whole bacteria cells. J Am Soc Mass Spectrom 14:342. doi:10.1016/s1044-0305(03)00065-5

    Article  CAS  PubMed  Google Scholar 

  • Wolfe RR (2006) The underappreciated role of muscle in health and disease. Am J Clin Nutr 84:475–482

    CAS  PubMed  Google Scholar 

  • Xu XY, Shen YB, Fu JJ, Liu F, GuoSZ Yang XM, Li JL (2011) Molecular cloning, characterization and expression patterns of HSP60 in the grass carp (Ctenopharyngodon idella). Fish Shellfish Imunol 31:864–870

    Article  CAS  Google Scholar 

  • Yarsan E, Yipel M (2013) The important terms of marine pollution “biomarkers and biomonitoring, Bioaccumulation, bioconcentration, biomagnification”. J Mol Biomark Diagn S1:003

    Google Scholar 

  • Zhou Q, Zhang J, Fu J, Shi J, Jiang G (2008) Biomonitoring: an appealing tool for assessment of metal pollution in the aquatic ecosystem. Anal Chim Acta 606:135–150

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Indian Council of Agricultural Research, New Delhi, under CIFRI Core Project No. FHE/ER/07/06/005 to BPM. The authors are thankful to the Director, CIFRI, Barrackpore, for the facilities and encouragement. The authors wish to record their sincere appreciation to the unknown reviewers for meticulously reading the manuscript and offering suggestions that helped in substantially improving it.

Conflict of interest

The authors declare that they have no conflict of interests.

Author information

Authors and Affiliations

  1. Proteomics Unit, Biochemistry Laboratory, Fishery Resource and Environmental Management Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata, 700 120, India

    Bimal Prasanna Mohanty, Tandrima Mitra, Sudeshna Banerjee, Soma Bhattacharjee, Arabinda Mahanty, Satabdi Ganguly & Dhanasekar Karunakaran

  2. KIIT School of Biotechnology, KIIT University, Bhubaneswar, 751 024, Odisha, India

    Gopal Krishna Purohit & Sasmita Mohanty

Authors
  1. Bimal Prasanna Mohanty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tandrima Mitra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sudeshna Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Soma Bhattacharjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Arabinda Mahanty
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Satabdi Ganguly
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gopal Krishna Purohit
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Dhanasekar Karunakaran
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Sasmita Mohanty
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bimal Prasanna Mohanty.

Electronic supplementary material

Below is the link to the electronic supplementary material.

10695_2015_46_MOESM1_ESM.pdf

ESM_1 The homology of mapped peptides with other species used for identification, the amino acid sequences of identified peptides and MALDI-TOF/TOF mass spectra of Rita rita (PDF 1378 kb)

10695_2015_46_MOESM2_ESM.xlsx

ESM_2 The homology of mapped peptides with other species used for identification, the amino acid sequences of identified peptides, i.e., Hsp90 sequence from LC-MS/MS (XLSX 117 kb)

10695_2015_46_MOESM3_ESM.xlsx

ESM_3 The homology of mapped peptides with other species used for identification, the amino acid sequences of identified peptides, i.e., α-actinin sequence from LC-MS/MS (XLSX 192 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mohanty, B.P., Mitra, T., Banerjee, S. et al. Proteomic profiling of white muscle from freshwater catfish Rita rita . Fish Physiol Biochem 41, 789–802 (2015). https://doi.org/10.1007/s10695-015-0046-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-015-0046-9

Keywords

Search

Navigation