Abstract
In this study, loach (Paramisgurnus dabryanus) were fed artificial diets containing 0.31 (control), 0.39, 0.48, 0.50 and 0.62 mg kg−1 of selenium (Se) for 60 days, respectively. Liver histopathology, hepatocyte ultrastructure, blood indices, biochemical parameters of liver functions and oxidative stress in the Se-treated loach were then assayed. The results showed the following: histopathological and ultrastructural lesions in liver were only observed in loach fed the 0.62 mg Se kg−1 diet; Haemoglobin and total protein were significantly increased in the 0.50 mg Se kg−1 group; albumin and high-density lipoprotein were increased significantly in the 0.48–0.50 mg Se kg−1 groups. However, white blood cell count was significantly decreased in the 0.48 mg Se kg−1 group; alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase were decreased in the 0.39–0.50 mg Se kg−1 groups. In liver tissue, the content of hydrogen peroxide was lower than that of controls in the 0.48–0.50 mg Se kg−1 groups, and the malondialdehyde level was lowest in the 0.48 mg Se kg−1 group. The activities of superoxide dismutase and glutathione peroxidase were significantly increased in the 0.50 mg Se kg−1 group; catalase and total antioxidant capacity were markedly increased in the 0.48–0.50 mg Se kg−1 group. These present results indicated that the dietary Se requirement for loach is 0.48–0.50 mg Se kg−1 diet.
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References
Abdel-Tawwab M, Wafeek M (2010) Response of Nile tilapia, Oreochromis niloticus (L.) to environmental cadmium toxicity during organic selenium supplementation. J World Aquacult Soc 41:106–114
Atencio L, Moreno I, Jos Á, Prieto AI, Moyano R, Blanco A, Cameán AM (2009) Effects of dietary selenium on the oxidative stress and pathological changes in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. Toxicon 53:269–282
Biasi F, Albano E, Chiarpotto E, Corongiu FP, Pronzato MA, Marinari UM, Parola M, Dianzani MU, Poli G (1991) In vivo and in vitro evidence concerning the role of lipid peroxidation in the mechanism of hepatocyte death due to carbon tetrachloride. Cell Biochem Funct 2:111–118
Borges A, Scotti LV, Siqueira DR, Zanini R, Do Amaral F, Jurinitz DF, Wassermann GF (2007) Changes in hematological and serum biochemical values in jundiá Rhamdia quelen due to sub-lethal toxicity of cypermethrin. Chemosphere 69:920–926
Cao YL, Ke T, Liu ZX, Lin M (2010) The studies on the effect of selenium on fish biology. Feed Ind Mag 31:52–56
Cho YS, Kim BS, Kim DS, Nam YK (2012) Modulation of warm-temperature-acclimation- associated 65-kDa protein genes (Wap65-1 and Wap65-2) in mud loach (Misgurnus mizolepis, Cypriniformes) liver in response to different stimulatory treatments. Fish Shellfish Immun 32:662–669
Deng YQ, Huang XL (2005) The studies on functions of selenium and its utilization in aquaculture. China Feed 16:22–24
Deng DF, Teh FC, Teh SJ (2008) Effect of dietary methylmercury and seleno-methionine on Sacramento splittail larvae. Sci Total Environ 407:197–203
Dutta HM, Adhikari S, Singh NK, Roy PK, Munshi JSD (1993) Histopathological changes induced by malathion in the liver of a freshwater catfish, Heteropneustes fossilis (Bloch). Bull Environ Contam Toxicol 51:895–900
Feng RH, Wang PH (2009) Studies on metabolism of serm lipids of rats with different doses of organic selenium. J Tianjing Med Univ 15:729–730
Gatlin DM, Wilson RP (1984) Dietary selenium requirement of fingerling channel catfish. J Nutr 114:627–633
Gusmão Affonso E, Costa Silva E, Tavares-Dias M, Menezes GC, Carvalho CSM, Silva Santiago Nunes É, Rebelo Ituassú D, Roubach R, Akifumi Ono E, Indrusiak Fim JD, Marcon JL (2007) Effect of high levels of dietary vitamin C on the blood responses of matrinxã (Brycon amazonicus). Comp Biochem Phys A 147:383–388
Hamilton SJ (2004) Review of selenium toxicity in the aquatic food chain. Sci Total Environ 326:1–31
Han D, Xie S, Liu M, Xiao X, Liu H, Zhu X, Yang Y (2011) The effects of dietary selenium on growth performances, oxidative stress and tissue selenium concentration of gibel carp (Carassius auratus gibelio). Aquacult Nutr 17:741–749
Hilton JW, Hodson PV, Slinger SJ (1980) The requirement and toxicity of selenium in rainbow trout (Salmo gairdneri). J Nutr 110:2527–2535
Hugla JL, Thomé JP (1999) Effects of polychlorinated biphenyls on liver ultrastructure, hepatic monooxygenases, and reproductive success in the barbel. Ecotox Environ Safe 42:265–273
Islinger M, Willimski D, Völkl A, Braunbeck T (2003) Effects of 17a-ethinylestradiol on the expression of three estrogen-responsive genes and cellular ultrastructure of liver and testes in male zebrafish. Aquat Toxicol 62:85–103
Kavitha C, Malarvizhi A, Kumaran SS, Ramesh M (2010) Toxicological effects of arsenate exposure on hematological, biochemical and liver transaminases activity in an Indian major carp, Catla catla. Food Chem Toxicol 48:2848–2854
Knook DL, Bosma A, Seifert WF (1995) Role of vitamin A in liver fibrosis. J Gastroen Hepatol 10:1440–1756
Köprücü SS, Köprücü K, Ural MS, İspir Ü, Pala M (2006) Acute toxicity of organophosphorous pesticide diazinon and its effects on behavior and some hematological parameters of fingerling European catfish (Silurus glanis L.). Pestic Biochem Phys 86:99–105
Li ZH, Velisek J, Zlabek V, Grabic R, Machova J, Kolarova J, Randak T (2010) Hepatic antioxidant status and hematological parameters in rainbow trout, Oncorhynchus mykiss, after chronic exposure to carbamazepine. Chem-Biol Interact 183:98–104
Li ZH, Velisek J, Zlabek V, Grabic R, Machova J, Kolarova J, Li P, Randak T (2011a) Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): effects on morphological indices, hematological parameters and antioxidant responses. J Hazard Mater 185:870–880
Li ZH, Zlabek V, Velisek J, Grabic R, Machova J, Kolarova J, Li P, Randak T (2011b) Acute toxicity of carbamazepine to juvenile rainbow trout (Oncorhynchus mykiss): effects on antioxidant responses, hematological parameters and hepatic EROD. Ecotox Environ Safe 74:319–327
Liang MQ, Wang JL, Chang Q, Liu XD, Mai KS (2006) Effects of dietary Se on growth performance and activities of related enzymes in juvenile Japanese sea bass (Lateolabrax japonicas). J Fish Sci China 13:1017–1022
Lin YH, Shiau SY (2005) Dietary selenium requirements of juvenile grouper, Epinephelus malabaricus. Aquaculture 250:356–363
Lin YH, Shiau SY (2007) The effects of dietary selenium on the oxidative stress of grouper, Epinephelus malabaricus, fed high copper. Aquaculture 267:38–43
Liu XJ, Luo Z, Li CH, Xiong BX, Zhao YH, Li XD (2011) Antioxidant responses, hepatic intermediary metabolism, histology and ultrastructure in Synechogobius hasta exposed to waterborne cadmium. Ecotox Environ Safe 74:1156–1163
Muscatello JR, Belknap AM, Janz DM (2008) Accumulation of selenium in aquatic systems downstream of a uranium mining operation in northern Saskatchewan, Canada. Environ Pollut 156:387–393
Ozardalı İ, Bitiren M, Karakılçık AZ, Zerin M, Aksoy N, Musa D (2004) Effects of selenium on histopathological and enzymatic changes in experimental liver injury of rats. Exp Toxicol Pathol 56:59–64
Parola M, Leonarduzzi G, Biasi F, Albano E, Biocca ME, Poli G, Dianzani MU (1992) Vitamin E dietary supplementation protects against carbon tetrachloride-induced chronic liver damage and cirrhosis. Hepatology 16:1014–1021
Qiu T, Xie P, Ke ZX, Li L, Guo LG (2007) In situ studies on physiological and biochemical responses of four fishes with different trophic levels to toxic cyanobacterial blooms in a large Chinese lake. Toxicon 50:365–376
Saravanan M, Karthika S, Malarvizhi A, Ramesh M (2011) Ecotoxicological impacts of clofibric acid and diclofenac in common carp (Cyprinus carpio) fingerlings: hematological, biochemical, ionoregulatory and enzymological responses. J Hazard Mater 195:188–194
Su CF, Li L, Li Q, Wen H, Wang FB (2008) Effects of dietary selenium on the anti-oxidation capability and histological structure of grass carp, Cenopharyngodon idellus. J Southwest China Norm Univ (Nat Sci E) 33:69–75
Sudová E, Piačková V, Kroupová H, Pijáček M, Svobodová Z (2009) The effect of praziquantel applied per os on selected haematological and biochemical indices in common carp (Cyprinus carpio L.). Fish Physiol Biochem 35:599–605
Svoboda M, Lusková V, Drastichová J, Žlábek V (2001) The effect of diazinon on haematological indices of common carp (Cyprinus carpio L.). Acta Vet Brno 70:457–465
Svobodova Z, Pravda D, Palackova J (1991) Unified methods of haematological examination of fish. Research Institute of Fish Culture and Hydrobiology, Vodnany, Methods No. 20, p 31
Sylvie BR, Pairault C, Vernet G, Boulekbache H (1996) Effect of lindane on the ultrastructure of the liver of the rainbow trout, Oncorhynchus mykiss, sac-fry. Chemosphere 33:2065–2079
Talas ZS, Orun I, Ozdemir I, Erdogan K, Alkan A, Yılmaz I (2008) Antioxidative role of selenium against the toxic effect of heavy metals (Cd2+, Cr3+)on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792). Fish Physiol Biochem 34:217–222
Tashjian DH, Teh SJ, Sogomonyan A, Hung SSO (2006) Bioaccumulation and chronic toxicity of dietary L-selenomethionine in juvenile white sturgeon (Acipenser transmontanus). Aquat Toxicol 79:401–409
Teh SJ, Deng X, Deng DF, Teh FC, Hung SS, Fan TW, Liu J, Higashi RM (2004) Chronic effects of dietary selenium on juvenile Sacramento splittail (Pogonichthys amcrolepidotus). Environ Sci Technol 38:6085–6093
Van-Dyk JC, Pieterse GM, Van-Vuren JHJ (2007) Histological changes in the liver of Oreochromis mossambicus (Cichlidae) after exposure to cadmium and zinc. Ecotox Environ Safe 66:432–440
Velisek J, Stara A, Kolarova J, Svobodova Z (2011) Biochemical, physiological and morfological responses in common carp (Cyprinus carpio L.) after long-term exposure to terbutryn in real environmental concentration. Pestic Biochem Phys 100:305–313
Velisek J, Stara A, Machova J, Svobodova Z (2012) Effects of long-term exposure to simazine in real concentrations on common carp (Cyprinus carpio L.). Ecotox Environ Safe 76:79–86
Wang C, Lovell RT (1997) Organic selenium sources, selenomethionine and selenoyeast, have higher bioavailability than an inorganic selenium source, sodium selenite, in diets for channel catfish (Ictalurus punctatus). Aquaculture 152:223–234
Watanabe T, Kiron V, Satoh S (1997) Trace minerals in fish nutrition. Aquaculture 151:185–207
Wilber CG (1980) Toxicology of selenium: a review. Clin Toxicol 17:171–230
Xia XH, Zhao J, Du QY, Zhi JH, Chang ZJ (2011) Cloning and identification of a female-specific DNA marker in Paramisgurnus dabryanus. Fish Physiol Biochem 37:53–59
Xie QX, Zhu XL, Lu QZ (1996) The role of sodium selenite in protection of primary hepatocytes and liver against carbon tetrachloride toxicity in grass carp. Acta Hydrobiol Sin 20:229–235
Yang YL, He ZA (2007) Laboratory diagnosis of clinical liver disease. China Tradit Chinese Med Press, Beijing
You LJ, Zhao MM, Liu RH, Regenstein JM (2011) Antioxidant and antiproliferative activities of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion. J Agr Food Chem 59:7948–7953
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This work was supported by Science Fund of Jiangsu Province (BE2012354) and by Aquatic Three Project of Jiangsu Province (PJ2011-62, DY2012-3).
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Hao, X., Ling, Q. & Hong, F. Effects of dietary selenium on the pathological changes and oxidative stress in loach (Paramisgurnus dabryanus). Fish Physiol Biochem 40, 1313–1323 (2014). https://doi.org/10.1007/s10695-014-9926-7
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DOI: https://doi.org/10.1007/s10695-014-9926-7