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Fish Physiology and Biochemistry

, Volume 41, Issue 1, pp 61–71 | Cite as

Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea

  • Muhammad Badruzzaman
  • Satoshi Imamura
  • Yuki Takeuchi
  • Taro Ikegami
  • Akihiro Takemura
Article

Abstract

In the neuroendocrine system controlling fish reproduction, dopamine (DA) acts as a gonadotropin inhibitory factor and plays a role in regulating gonadal development of certain species. The present study examined the effects of chemical destruction of dopaminergic neurons in the brain on DA production and ovarian development in the sapphire devil Chrysiptera cyanea, a reef-associated damselfish. The avidin–biotin–peroxidase complex method using an antibody against tyrosine hydroxylase (TH), a critical enzyme in the DA synthesis pathway, identified a population of dopaminergic neurons with somata in the anteroventral preoptic nucleus of the diencephalon and fibers terminating in the proximal pars distalis of the pituitary. Maintaining fish in seawater containing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at 0.02 and 0.2 µg/mL for 2 days resulted in decreases in DA, 3,4-dihydroxyphenylacetic acid (DOPAC; DA metabolite), and DA metabolic rate in the whole brain. The number of TH-positive neurons in the diencephalon decreased after 0.02 µg/mL MPTP treatment for 2 days. These results suggest that MPTP treatment destroys TH-positive neurons in the diencephalon, thereby decreasing the synthesis and release of DA from the brain. This treatment rescued ovarian development in fish with artificially retracted ovaries during the spawning season. The gonadosomatic index of MPTP-treated fish 5 and 7 days after treatment was significantly higher than that of control fish. Oocytes in the vitellogenic stages were observed in the ovaries of MPTP-treated fish, but not in control fish. These results suggest that DA in the brain drives ovarian development in the sapphire devil.

Keywords

Damselfish Dopamine MPTP Ovary Reproduction Tyrosine hydroxylase Vitellogenesis 

Notes

Acknowledgments

We thank the staff of Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, for their technical assistance. This study was supported in part by a Grant-in-Aid for Scientific Research (A) (JSPS KAKENHI Grant Number: 23248033) and for Challenging Exploratory Research (JSPS KAKENHI Grant Number: 25660170) from the Japan Society for the Promotion of Science (JSPS) to AT.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Muhammad Badruzzaman
    • 1
  • Satoshi Imamura
    • 1
  • Yuki Takeuchi
    • 1
  • Taro Ikegami
    • 1
  • Akihiro Takemura
    • 1
  1. 1.Department of Chemistry, Biology and Marine ScienceUniversity of the RyukyusNishiharaJapan

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