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Effect of vitamin C dietary supplementation in reducing the alterations induced by fenitrothion in Oreochromis niloticus

Fish Physiology and Biochemistry volume 40, pages 787–796 (2014)Cite this article

Abstract

The objective of this study was to investigate the dietary effect of vitamin C in amelioration some of studied alterations induced by fenitrothion in Nile tilapia (Oreochromis niloticus). Nile tilapia was exposed to sub-lethal concentration of fenitrothion 0.04 mg/l (96 h LC50 value was 0.8 mg/l), and basal diet was supplemented with two different dose of vitamin C (500 and 1200 mg/kg B wt/day) for 30 days. Vitamin C supplemented groups showed significant decrease in plasma cortisol and glucose level, enzymes activity of liver and gills (catalase, glutathione-S-transferase and superoxide dismutase) and % of tail DNA damage compared to exposed group. Moreover, fish revealed significant increase in total plasma protein, albumin, globulin and A/G ratio. High dose of vitamin C dietary supplementation (1200 mg/kg B wt/day) returns these parameters to its normal levels with no significant difference compared to non exposed control group. These results indicated that incorporation of high dose of vitamin C (1200 mg) in aqua feed for 30 days could be potentially less expensive and effective in reducing the alterations induced by fenitrothion in Nile tilapia.

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References

  • Abalaka SE, Esievo KAN, Shoyinka SVO (2011) Evaluation of biochemical changes in Clarias gariepinus adults exposed to aqueous and ethanolic extracts of Parkia biglobosa pods. Afr J Biotechnol 10:234–240. doi:10.5897/AJB10.331

    Google Scholar 

  • Alazemi BM, Lewis JW, Andrews EB (1996) Gill damage in the freshwater fish Gnathonemus petersii (Family: Mormyridae) exposed to selected pollutants: an ultra structural study. Environ Technol 17:225–238. doi:10.1080/09593331708616381

    Article  CAS  Google Scholar 

  • Al-Ghanim KA (2012) Acute toxicity and effects of sub-lethal malathion exposure on biochemical and haematological parameters of Oreochromis niloticus. Sci Res Essays 7(16):1674–1680. doi:10.5897/SRE12.039

    CAS  Google Scholar 

  • Ali D, Nagpure NS, Kumar S, Kumar R, Kushwaha B, Lakra WS (2009) Assessment of gentoxic and mutogenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single cell gel electrophoresis. Food Chem Toxicol 47:650–656. doi:10.1016/j.fct.2008.12.021

    Article  CAS  PubMed  Google Scholar 

  • American Public Health Association, APHA (1998) Standard method for the examination of water and wastewater. American Public Health Association, APHA, Washington

    Google Scholar 

  • Ameur WB, Lapuente JD, El Megdiche Y, Barhoumi B, Trabelsi S, Camps L, Serret J, Romos-Lopez D, Gonzalez-Linares J, Driss MR, Borras M (2012) Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia). Mar Pollut Bull 64:241–251. doi:10.1016/j.marpolbul.2011.11.026

    Article  PubMed  Google Scholar 

  • Amin KA, Hashem KS (2012) Deltamethrin induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol. Vet Res 45:1–8. doi:10.1186/1746-6148-8-45

    Google Scholar 

  • Barcellos LJG, Nicolaiewsky S, De Souza SMG, Lulhier F (1999) Plasmatic levels of cortisol in the response to acute stress in Nile tilapia, Oreochromis niloticus (L.), previously exposed to chronic stress. Aquac Res 30:437–444. doi:10.1046/j.1365-2109.1999.00348.x

    Article  Google Scholar 

  • Bendich A (1990) Antioxidant micro nutrients and immune responses. In: Bendich A, Butterworth CE (eds) Micronutrients and immune functions, vol 87. New York Academy of Science, New York, pp 168–180

    Chapter  Google Scholar 

  • Benli ACK, Ozkul A (2010) Acute toxicity and histopathological effects of sub-lethal fenitrothion on Nile tilapia, Oreochromis niloticus. Pestic Biochem Physiol 97:32–35. doi:10.1016/j.pestbp.2009.12.001

    Article  CAS  Google Scholar 

  • Bradbury SP, Makim JR, Coats JR (1987) Physiological responses of rainbow trout, Salmo gairdneri to acute fenvalerate intoxication. Pestic Biochem Physiol 27:275–285. doi:10.1016/0048-3575(87)90057-5

    Article  CAS  Google Scholar 

  • Brinelli A, Provini A (2004) Risk for human health of some POPs due to fish from Lake Iseo. Ecotoxicol Environ Saf 58(1):139–145. doi:10.1016/j.ecoenv.2003.09.014

    Article  Google Scholar 

  • Brown JA (1993) Endocrine responses to environmental pollutants. In: Rankin JC, Jensen FB (eds) Fish ecophysiology. Chapman & Hall, London, pp 276–296

    Chapter  Google Scholar 

  • Cericato L, Neto JG, Fagundes M, Kreutz LC, Quevedo RM, Finco J, Da Rosa JG, Koakoski G, Centenaro L, Potter E, Anziliero D, Barcellos LJ (2008) Cortisol response to acute stress in jundiá Rhamdia quelen acutely exposed to sublethal concentration of agrichemicals. Comp Biochem Physiol 148:281–286. doi:10.1016/j.cbpc.2008.06.008

    Google Scholar 

  • Cheung CCC, Zheng GJ, Li AMY, Richardson BJ, Lam PKS (2001) Relationships between tissue concentration of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquat Toxicol 52:189–203. doi:10.1016/S0166-445X(00)00145-4

    Article  CAS  PubMed  Google Scholar 

  • Dabrowska H, Dabrowski K, Meyer-Burgdorff K, Hanke W, Gunther KD (1991) The effect of large doses of vitamin C and magnesium on stress responses in common carp Cyprinus carpio. Comp Biochem Physiol 99A:681–685. doi:10.1016/0300-9629(91)90150-B

    Article  CAS  Google Scholar 

  • Datta M, Kaviraj A (2003) Ascorbic acid supplementation of diet for reduction of deltamethrin induced stress in freshwater catfish Clarias gariepinus. Chemosphere 53:883–888. doi:10.1016/S0045-6535(03)00557-5

    Article  CAS  PubMed  Google Scholar 

  • Di Giulio RT, Washburn PC, Wenning RJ, Winston GW, Jewell CS (1989) Biochemical responses in aquatic animals: a review of oxidative stress. Environ Toxicol Chem 8:1103–1123. doi:10.1002/etc.5620081203

    Article  Google Scholar 

  • Dogan D, Can C, Kocyigit A, Dikilitas M, Taskin A, Bilinc H (2011) Dimethoate-induced oxidative stress and DNA damage in Oncorhynchus mykiss. Chemosphere 84:39–46. doi:10.1016/j.chemosphere.2011.02.087

    Article  CAS  PubMed  Google Scholar 

  • Eipper BA, Milgram SL, Husten EJ, Yun HY, Mains RE (1993) Peptidylglycine alpha-amidating monooxygenase: a multifunctional protein with catalytic, processing, and routing domains. Protein Sci 2(4):489–497. doi:10.1002/pro.5560020401

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Figueiredo-Fernandes A, Ferreira-Cardoso JV, Gar-cia-Santos S, Monteiro SM, Carrola J, Matos P, Fontainhas-Fernandes A (2007) Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus, exposed to waterborne copper. Pesqui Vet Bras 27(3):103–109. doi:10.1590/S0100-736X2007000300004

    Article  Google Scholar 

  • Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, pp 50–80

    Google Scholar 

  • Firat O, Cogun Y, Yuzereroglu TA, Gok G, Firat O, Kargin F, Kotemen Y (2011) A comparative study on the effect of pesticide (Cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiol Biochem 37(3):657–666. doi:10.1007/s10695-011-9466-3

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Garcia-Santos S, Fontainhas-Fernandes A, Wilson JM (2006) Cadmium tolerance in the Nile tilapia (Oreochromis niloticus) following acute exposure: assessment of some ionoregulatory parameters. Environ Toxicol 1:33–46. doi:10.1002/tox.20152

    Article  Google Scholar 

  • Gernhöfer M, Pawet M, Schramm M, Müller E, Triebskorn R (2001) Ultrastructural biomarkers as tools to characterize the health status of fish in contaminated stream. J Aquat Ecosyst Stress Recover 8:241–260. doi:10.1023/A:1012958804442

    Article  Google Scholar 

  • Gill TS, Leitner G, Porta S, Epple H (1993) Response of plasma cortisol to environmental cadmium in the eel, Anguilla rostraralesueur. J Comp Biochem Physiol 104:489–495. doi:10.1016/0742-8413(93)90023-E

    CAS  Google Scholar 

  • Habig WH, Pabst MN, Bjacoby W (1974) Glutathion, transferase, the first enzymatic step in mercatpopunc acid formation. J Biol Chem 249:7130–7138

    CAS  PubMed  Google Scholar 

  • Halver JE (1995) Vitamin requirement study techniques. J Appl Ichthyol 11:215–224. doi:10.1111/j.1439-0426.1995.tb00021.x

    Article  CAS  Google Scholar 

  • Hardie LJ, Fletcher TC, Secombes CJ (1991) The effect of dietary vitamin C on the immune response of the Atlantic salmon (Salmo salar L.). Aquaculture 21:39–53. doi:10.1016/0044-8486(91)90087-N

    Google Scholar 

  • Iwama GK, Vijayan MM, Forsyth RB, Ackerman PA (1999) Heat shock proteins and physiological stress in fish. Am Zool 39:901–909. doi:10.1093/icb/39.6.901

    CAS  Google Scholar 

  • Jacobes E, Kasten PL, Demoott WR, Wolfson WL (1990) Laboratory test handbook, 2nd edn. Williams and Wilkins, Baltimore

    Google Scholar 

  • Kaplan A, Szabo LL (1983) Clinical chemistry: interpretation and techniques, 2nd edn. Lea & Febiger, Philadelphia, p 157

    Google Scholar 

  • Knobil K (1980) Hormonal researches. Prog Horm Res 36:52–58

    Google Scholar 

  • Korkmaz N, Cengiz El, Unlu E, Uysal E, Yanar M (2009) Cypermethrin-induced histopathological and biochemical changes in Nile tilapia (Oreochromis niloticus) and the protective and recuperative effect of ascorbic acid. Environ Toxicol Pharmacol 28:198–205. doi:10.1016/j.etap.2009.04.004

    Article  CAS  PubMed  Google Scholar 

  • Kumar R, Nagpure NS, Kushwaha B, Srivastava SK, Lakra WS (2010) Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus and comet assay. Arch Environ Contam Toxicol 58(1):123–130. doi:10.1007/s00244-009-9354-3

    Article  CAS  PubMed  Google Scholar 

  • Kumar N, Antony Jesu Prabhu P, Pal AK, Remya S, Aklakur Md, Rana RS, Gupta S, Raman RP, Jadhao SB (2011) Anti-oxidant and immuno-hematological status of tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pestic Biochem Physiol 99:45–52. doi:10.1016/j.pestbp.2010.10.003

    Article  CAS  Google Scholar 

  • Livingston DR (2001) Contaminate-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar Pollut Bull 42:656–666. doi:10.1016/S0025-326X(01)00060-1

    Article  Google Scholar 

  • May JM (2000) How dose ascorbic acid prevent endothelium dysfunction? Free Radic Biol Med 28:14–21

    Article  Google Scholar 

  • Mazeoud MM, Mazeoud F, Donaldson EM (1977) Primary and secondary effect of stress in fish, some new data with a general review. Trans Am Fish Soc 106:201–212. doi:10.1577/1548-8659(1977)106<201

    Article  Google Scholar 

  • Meister RT (1994) Farm chemicals handbook. Meister publishing Co., Willoughby

    Google Scholar 

  • Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175. http://www.jbc.org/content/247/10/3170

  • Mitchelmore CL, Chipman JK (1998) DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat Res 399:135–147

    Article  CAS  PubMed  Google Scholar 

  • Monteiro DA, Almeida JA, Rantin FT, Kalinin AL (2006) Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus exposed to organophosphorus insecticide folisuper 600 (methyl parathion). Comp Biochem Physiol C 143:141–149. doi:10.1016/j.cbpc.2006.01.004

    Article  Google Scholar 

  • Montero D, Marrero M, Izquierdo MS, Robaina L, Vergara JM, Tort L (1999) Effect of vitamin E and C dietary supplementation on some immune parameters of gilthead seabream (Sparus aurata) juveniles subjected to crowding stress. Aquaculture 171:269–278. doi:10.1016/S0044-8486(98)00387-1

    Article  CAS  Google Scholar 

  • Nayak AK, Das BK, Demoott WR, Wolfson WL (2004) The immunosuppressive effect of apermethrin on indian major carp, rohu (Labeo rohita). Fish Shellfish Immunol 16(1):41–50. doi:10.1016/S1050-4648(03)00029-9

    Article  CAS  PubMed  Google Scholar 

  • Niki E (1991) Action of ascorbic acid as a scavenger of active and stable oxygen radicals. Am J Clin Nutr 54:11195–245. http://www.ncbi.nlm.nih.gov/pubmed/1962557

    Google Scholar 

  • Nogueira P, Pacheco M, Pereira ML, Mendo S, Rotchell S (2009) Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination. Environ Pollut 158:1783–1790. doi:10.1016/j.envpol.2009.11.007

    Article  PubMed  Google Scholar 

  • Ohe T, Watanabe T, Wakabayashi K (2004) Mutagenes in surface water: a review. Mutat Res 567:109–149. doi:10.1016/j.mrrev.2004.08.003

    Article  CAS  PubMed  Google Scholar 

  • Özcan Oruc E (2010) Oxidative stress, steroid hormone concentration and acetylcholinesterase activity in Oreochromis niloticus exposed to chlorpyrifos. Pestic Biochem Physiol 96:160–166. doi:10.1016/j.pestbp.2009.11.005

    Article  Google Scholar 

  • Pandey S, Nagpure NS, Kumar R, Sharma S, Srivastava SK, Verma MS (2006) Genotoxicity evaluation of acute doses of endosulfan to freshwater teleost Channa punctatus (Bloch) by alkaline single cell gel electrophoresis. Ecotoxicol Environ Saf 65:56–61. doi:10.1016/j.ecoenv.2005.06.007

    Article  CAS  PubMed  Google Scholar 

  • Pandey AK, Nagpure NS, Trivedi SP, Kumar R, Kushwaha B (2011) Profenofos induced DNA damage in freshwater fish, Channa punctatus (Bloch) using alkaline single cell gel electrophoresis. Mutat Res 726:209–214. doi:10.1016/j.mrgentox.2011.09.011

    Article  CAS  PubMed  Google Scholar 

  • Pickering AD (1981) Introduction: the concept of biological stress. In: Pickering AD (ed) Stress and fish. Academic Press, London

    Google Scholar 

  • Ramdas SB (2013) Ascorbic effect on the endosulphan induced alterations in blood glucose level of the freshwater fish, Channa orientalis (Schneider). Central Eur J Exp Biol 2:1–3

    Google Scholar 

  • Roy RN, Guma BC (1958) Species difference in regard to the biosynthesis of ascorbic acid. Nature 182:319–320. doi:10.1038/182319a0

    Article  CAS  PubMed  Google Scholar 

  • Sancho E, Ferrando MD, Gamon M, Andreu-Moliner E (1998) Uptake and elimination kinetics of a pesticide in the liver of the European eel. J Environ Sci Health B 33:83–98. doi:10.1080/03601239809373131

    Article  CAS  PubMed  Google Scholar 

  • Sarikaya R, Selve M, Erkoc F (2004) Investigation of acute toxicity of fenitrothion on peppered corydoras (Corydoras paleatus) (Jenyns, 1842). Chemosphere 56:697–700. doi:10.1016/j.chemosphere.2004.04.008

    Article  CAS  PubMed  Google Scholar 

  • Sepici-Dincel A, Benli ACK, Selvi M, Sarikaya R, Sahin D, Ozkull A, Erkoc F (2009) Sublethal cyfluthrin toxicity to carp (Cyprinus carpio L.) fingerlings: biochemical, haematological, histopathological alteration. Ecotoxicol Environ Saf 72:1433–1439. doi:10.1016/j.ecoenv.2009.01.008

    Article  CAS  PubMed  Google Scholar 

  • Sies H (1986) Biochemistry of oxidative stress. Angew Chem Int Ed 25:1058–1071

    Article  Google Scholar 

  • Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191. doi:10.1016/0014-4827(88)90265-0

    Article  CAS  PubMed  Google Scholar 

  • Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47:389–394. doi:10.1016/0003-2697(72)90132-7

    Article  CAS  PubMed  Google Scholar 

  • Thangavel P, Sumathiral K, Karthikeyan S, Ramaswamy M (2005) Endocrine response of the freshwater teleost, Sarotherodon mossambicus (peters) to dimecron exposure. Chemosphere 61(8):1083–1092. doi:10.1016/j.chemosphere.2005.03.045

    Article  CAS  PubMed  Google Scholar 

  • Thomaz JM, Martins ND, Monteiro DA, Rantin FT, Kalinin AL (2009) Cardio-respiratory function and oxidative stress biomarkers in Nile tilapia exposed to the organophosphate insecticides trichlorfon (NEGUVON). Ecotoxicol Environ Saf 72:1413–1424. doi:10.1016/j.ecoenv.2008.11.003

    Article  CAS  PubMed  Google Scholar 

  • Tietz NW (1994) Textbook of clinical chemistry, 2nd edn. Saunders WB. Company, Philadelphia

  • Trinder P (1969) Determination of blood glucose using 4-Aminophenazone as oxygen acceptor. J Clin Pathol 22(2):246

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tripathi G, Shasmal J (2010) Reparation of chlorpyrifos-induced impairment by thyroxine and vitamin C in fish. Ecotoxicol Environ Saf 73:1397–1401. doi:10.1016/j.ecoenv.2010.07.022

    Article  CAS  PubMed  Google Scholar 

  • Vani T, Saharan N, Mukherjee SC, Ranjan R, Kumar R, Brahmchari RK (2011) Deltamethrin induced alteration of haematological and biochemical parameters in fingerlings of Catla catla (Ham.) and their amelioration by dietary supplement of vitamin C. Pestic Biochem Physiol 101:16–20. doi:10.1016/j.pestbp.2011.05.007

    Article  CAS  Google Scholar 

  • Wendelaar Bonga SE (1997) The stress response in fish. Physiol Rev 77:591–625

    CAS  PubMed  Google Scholar 

  • Wilhelm-Filho D, Giulivi C, Boveris A (1993) Antioxidant defences in marine fish. I. Teleosts. Comp Biochem Physiol C 106:409–413. doi:10.1016/0742-8413(93)90154-D

    Google Scholar 

  • Winkalar EU, Santosh TRM, Machdo-Neto JG, Martinez CBR (2007) Acute lethal and sublethal effects of neem leaf extracts on neotropical freshwater fish. Prochilodus lineatus. Comp Biochem Physiol C 145:236–244

    Google Scholar 

  • Worthing CR (1987) The pesticide manual: a world compendium, 8th edn. Published by the British Crop Protection Council, Hampshire, UK

  • Yang Y, Cheng JZ, Singhal SS, Sain M, Pandya U, Awasthu S, Awasthi YC (2001) Role of glutathione S-transverse in protection against lipid peroxidation. Overexpression of hGSTA2-2 in K562 cells protects against hydrogen peroxide-induced apoptosis and inhibits JNK and caspase 3 activation. J Biol Chem 276:19220–19230. doi:10.1074/jbc.M100551200

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are thankful for Head of Department of Fish Diseases and Management, Faculty of Vet. Med. Benha University, Egypt Prof. Dr. Adel A. Shaheen for his continuous advice.

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  1. Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Kalubia, 13736, Egypt

    Eman A. Abd El-Gawad

  2. Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Kalubia, 13736, Egypt

    Omnia M. Abdel Hamid

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Correspondence to Eman A. Abd El-Gawad.

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Abd El-Gawad, E.A., Abdel Hamid, O.M. Effect of vitamin C dietary supplementation in reducing the alterations induced by fenitrothion in Oreochromis niloticus . Fish Physiol Biochem 40, 787–796 (2014). https://doi.org/10.1007/s10695-013-9885-4

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Keywords

  • Vitamin C
  • Fenitrothion
  • Antioxidant enzymes
  • Biochemical parameters
  • DNA damage
  • Nile tilapia