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Domain composition of rhamnose-binding lectin from shishamo smelt eggs and its carbohydrate-binding profiles

Fish Physiology and Biochemistry volume 39, pages 1619–1630 (2013)Cite this article

Abstract

Osmerus (Spirinchus) lanceolatus egg lectin (OLL) is a member of the rhamnose-binding lectin (RBL) family which is mainly found in aqueous beings. cDNA of OLL was cloned, and its genomic architecture was revealed. The deduced amino acid (aa) sequence indicated that OLL was composed of 213 aa including 95 aa of domain N and 97 aa of domain C. N and C showed 73 % sequence identity and contained both -ANYGR- and -DPC-KYL-peptide motifs which are conserved in most of the RBL carbohydrate recognition domains. The calculated molecular mass of mature OLL was 20,852, consistent with the result, and 20,677.716, from mass spectrometry. OLL was encoded by eight exons: exons 1 and 2 for a signal peptide; exons 3–5 and 6–8 for N- and C-domains, respectively. Surface plasmon resonance spectrometric analyses revealed that OLL showed comparable affinity for Galα- and β-linkages, whereas Silurus asotus lectin (SAL), a catfish RBL, bound preferentially to α-linkages of neoglycoproteins. The Kd values of OLL and SAL against globotriaosylceramide (Gb3) were 1.69 × 10−5 M for and 2.81 × 10−6 M, respectively. Thus, the carbohydrate recognition property of OLL is slightly different from that of SAL. On the other hand, frontal affinity chromatography revealed that both OLL and SAL interacted with only glycolipid-type oligosaccharides such as Gb3 trisaccharides, not with N-linked oligosaccharides. The domain composition of these RBLs and an analytical environment such as the “cluster effect” of a ligand might influence the binding between RBL and sugar chains.

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Abbreviations

RBL:

Rhamnose-binding lectin

OLL:

Osmerus lanceolatus lectin

SAL:

Silurus asotus lectin

SPR:

Surface plasmon resonance

FAC:

Frontal affinity chromatography

CRD:

Carbohydrate recognition domain

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Acknowledgments

A part of this work was supported by a Grant-in-Aid for Frontier Research Program from The Ministry of Education, Culture, Sports, Science, and Technology of Japan. We thank Dr. Stephen Anderson for editing of the manuscript.

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Authors and Affiliations

  1. Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, 981-8558, Japan

    Masahiro Hosono, Shigeki Sugawara, Takeo Tatsuta & Kazuo Nitta

  2. Department of Pediatrics, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan

    Toshiyuki Hikita

  3. Research Center for Medical Glycosciences, National Institute of Advanced Industrial Science and Technology, 1-1-1, Umezono, Tsukuba, Ibaraki, 305-8568, Japan

    Junko Kominami & Sachiko Nakamura-Tsuruta

  4. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, 1-1-1, Higashi, Tsukuba, Ibaraki, 305-8562, Japan

    Jun Hirabayashi

  5. Laboratory of Carbohydrate and Protein Chemistry, Department of Chemistry, Faculty of Science, University of Chittagong, Chittagong, 4331, Bangladesh

    Sarkar M. A. Kawsar

  6. Laboratory of Glycobiology and Marine Biochemistry, Department of Life and Environmental System Science, Graduate School of NanoBio Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, 236-0027, Japan

    Yasuhiro Ozeki

  7. Division of Biomembrane Research, Pacific Northwest Research Institute, Seattle, WA, 98122-4302, USA

    Sen-itiroh Hakomori

  8. Departments of Pathobiology and Microbiology, University of Washington, Seattle, WA, 98195, USA

    Sen-itiroh Hakomori

Authors
  1. Masahiro Hosono
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  2. Shigeki Sugawara
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  3. Takeo Tatsuta
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  4. Toshiyuki Hikita
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  5. Junko Kominami
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  6. Sachiko Nakamura-Tsuruta
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  7. Jun Hirabayashi
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  8. Sarkar M. A. Kawsar
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  9. Yasuhiro Ozeki
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  10. Sen-itiroh Hakomori
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  11. Kazuo Nitta
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Corresponding author

Correspondence to Masahiro Hosono.

Additional information

The nucleotide sequence reported in this paper has been submitted to the DDBJ/EMBL/GenBank Data Bank with accession number AB047559 and AB444586.

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Hosono, M., Sugawara, S., Tatsuta, T. et al. Domain composition of rhamnose-binding lectin from shishamo smelt eggs and its carbohydrate-binding profiles. Fish Physiol Biochem 39, 1619–1630 (2013). https://doi.org/10.1007/s10695-013-9814-6

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