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Molecular cloning of grass carp (Ctenopharyngodon idellus) T-bet and GATA-3, and their expression profiles with IFN-γ in response to grass carp reovirus (GCRV) infection

Fish Physiology and Biochemistry volume 39, pages 793–805 (2013)Cite this article

Abstract

Both T-bet and GATA-3, Th1/Th2 lineage-specific transcription factors, play important roles in the development of T cells and Th1/Th2 differentiation. In this study, T-bet and GATA-3 genes were cloned from grass carp (Ctenopharyngodon idellus). The putative primary structure of the polypeptide deduced from the cDNA sequence of grass carp T-bet contained 608 aa, which possessed a T-box DNA binding domain. The putative primary structure of the polypeptide deduced from the cDNA sequence of grass carp GATA-3 contained 396 aa, which possessed two consensus zinc finger domains (C-X(2)-C-X(17)-C-X(2)-C). The YxKxHxxxRP motif, KRRLSA and LMEKs/n sequences were also conserved in this GATA-3. Phylogenetic analysis indicated that grass carp T-bet and GATA-3 group with their known counterparts with zebrafish T-bet and GATA-3 as the closest neighbor, respectively. RT-qPCR results showed that grass carp T-bet gene was highly expressed in head kidney, followed by spleen, and low expressed in gill, liver, kidney, and intestine, while GATA-3 gene was highly expressed in intestine, followed by spleen, and low expressed in gill, liver, kidney, and head kidney. Grass carp is one of the “four important domestic fish” in China and often infected by grass carp reovirus (GCRV). As yet, there is no evidence that T-bet and GATA-3 (Th1/Th2 subsets) are involved in anti-virus immune of teleost fish. In this study, by RT-qPCR, we analyzed the expression dynamics of grass carp T-bet and GATA-3 genes with IFN-γ gene in response to GCRV infection for the first time. The expression dynamics showed that three genes might be crucially modulated by in vivo GCRV infection: (1) GCRV mainly induced a T-bet expression profile comparing to the GATA-3 expression, while the higher expression profiles of IFN-γ correlated with the up-regulation of T-bet; (2) T-bet/IFN-γ and GATA-3 expression changes suggest that in GCRV-infected grass carp, the common immune state of head kidney further heightens, whereas the common physiological state of intestine transforms to an anti-virus immune state. From this finding, we realize that GCRV mainly induces a Th1 response, and Th1 cell-mediated recognition mechanisms play very important roles in anti-virus cellular immune of grass carp.

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Acknowledgments

This work was supported by the National Basic Research Program of China (Grant No. 2009CB118704), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (Grant No. 2012FB19), and the National Natural Science Foundation of China (Grant No. 30871912). We also thank Prof. Xudong Xu and Zhan Yin (Institute of Hydrobiology, Chinese Academy of Sciences) for their technique supports.

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Authors and Affiliations

  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Lu Wang, Na Shang, Hong Feng, Qionglin Guo & Heping Dai

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100039, China

    Lu Wang & Na Shang

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  1. Lu Wang
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  2. Na Shang
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  3. Hong Feng
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  4. Qionglin Guo
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  5. Heping Dai
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Correspondence to Qionglin Guo or Heping Dai.

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Wang, L., Shang, N., Feng, H. et al. Molecular cloning of grass carp (Ctenopharyngodon idellus) T-bet and GATA-3, and their expression profiles with IFN-γ in response to grass carp reovirus (GCRV) infection. Fish Physiol Biochem 39, 793–805 (2013). https://doi.org/10.1007/s10695-012-9741-y

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  • DOI: https://doi.org/10.1007/s10695-012-9741-y

Keywords

  • T-bet/GATA-3
  • Th1/Th2 subsets
  • IFN-γ
  • Th1 response
  • Grass carp reovirus (GCRV)
  • Grass carp