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Heat shock protein 70 kDa (HSP70) increase in sea bass (Dicentrarchus labrax, L 1758) thymus after vaccination against Listonella anguillarum

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Abstract

Heat shock proteins 70 kDa (HSP70) and apoptosis were investigated in thymus of sea bass juveniles (Dicentrarchus labrax) subsequently to a vaccination against Listonella (syn. Vibrio) anguillarum. HSP70 expression was measured by immunohistochemistry and immunoenzymatic methods, resulting in increase in HSP70 after bath immunization and persistent in fish exposed to an intraperitoneal (i.p.) booster. The HSP70 increase in thymus was suggested as induction in lymphocytic cells, to be related to immune system stimulation after vaccination. However, a thymic recruitment of lymphocyte subpopulations, characterized by higher expression of HSP70, was also hypothesized after vaccination. No apparent relationships were found between HSP70 and apoptosis. In fact, the vaccination did not modulate the apoptosis response, as measured by TUNEL assay and by immunohistochemistry for active caspase-3 expression. The lack of apoptosis effects could be ascribed to the use of inactivated bacteria that appeared not able to interfere with programmed cell death mechanisms. This manuscript aims to contribute to the knowledge of some biochemical features underlying the immunization, with a particular emphasis on the modulation of HSP70. However, further parameters involved in innate/adaptative immunity and apoptosis pathways have to be taken into account to well establish the functional role of HSP70 in fish vaccination.

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Acknowledgments

This study was funded by the Italian Ministry of Education, University and Research (MIUR-PRIN 2006) “Modulation of immune response in sea bass (D. labrax L.) submitted to infection with a bacterial agent and vaccination.”

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  1. Dipartimento di Scienze Biomediche Comparate, P.zza A. Moro 45, 64100, Teramo, Italy

    Francesco Mosca, Daniela Malatesta, Anastasia Falconi & Pietro G. Tiscar

  2. Dipartimento di Ecologia e Biologia, Via S. Maria in Gradi 4, 1100, Viterbo, Italy

    Nicla Romano, Giuseppina Ceccarelli & Andrea Brunetti

  3. Dipartimento di Scienze degli Alimenti, Via Sondrio 2, 33100, Udine, Italy

    Chiara Bulfon, Donatella Volpatti & Marco Galeotti

  4. Dipartimento di Scienze della Vita e Biotecnologie, Via Borsari 43, 44121, Ferrara, Italy

    Luigi Abelli

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  1. Francesco Mosca
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Correspondence to Pietro G. Tiscar.

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Francesco Mosca and Nicla Romano equally contributed to this paper.

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Mosca, F., Romano, N., Malatesta, D. et al. Heat shock protein 70 kDa (HSP70) increase in sea bass (Dicentrarchus labrax, L 1758) thymus after vaccination against Listonella anguillarum . Fish Physiol Biochem 39, 615–626 (2013). https://doi.org/10.1007/s10695-012-9724-z

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