Molecular cloning, characterization, and gene expression of the androgen receptor in the large yellow croaker, Larimichthys crocea
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Androgens mediate a wide range of physiological responses and developmental processes in vertebrates, involving both reproductive and nonreproductive systems. The activity of androgens is mediated by the androgen receptor (AR), a member of the nuclear receptor superfamily. In this study, an AR gene was cloned from the large yellow croaker (Larimichthys crocea) for the first time. qRT-PCR revealed ubiquitous expression of AR in all adult tissues examined, with higher expression in the gonad and liver of both sexes and highest expression in the blastula stage of embryonic development. Using in situ hybridization, we detected positive signals of AR in the spermatogonium, spermatocyte, spermatid, and spermatozoon during spermatogenesis, in the cytoplasm of all oocytes during oogenesis and in the follicle cells of stage IV oocytes. Our findings support the important role that AR plays in gametogenesis, gonadal development, and the early stages of embryonic development.
KeywordsLarimichthys crocea AR Gametogenesis Gonadal development Embryonic development
This project was supported by the National Natural Science Foundation of China (No. 30600467) and the Innovation Team Foundation of Jimei University (2010A001).
- Chen H, Chen W, Lin GW, Liu ZK, Xie YQ, Gao ZG (2007) The morphological characteristics and growth pattern of cage-cultured large yellow croaker (Larimichthys crocea) in Guanjing-Yang population. Hai Yang Ke Xue 29:331–336 (In Chinese)Google Scholar
- Fang YQ, Weng YZ, Zhou J, Liu JF, Xie FJ (2000) Study on earlier gonadal maturation in cultured large yellow croaker, Pseudosciaena crocea I. Status of gonadal development in culture of net cages. Tai Wan Hai Xia 19:354–359 (In Chinese)Google Scholar
- Lin D, Zhan J, Luo J, Zheng ZY, Shi XW (1992) Studies on the gonadal development and the annual reproductive cycle of the cultured large yellow croaker, Pseudosciaena crocea (Richardson). J Fujian Normal Univ (Nat Sci) 8:81–87 (In Chinese)Google Scholar
- Lutz LB, Cole LM, Gupta MK, Kwist KW, Auchus RJ, Hammes SR (2001) Evidence that androgens are the primary steroids produced by Xenopus laevis ovaries and may signal through the classical androgen receptor to promote oocyte maturation. Proc Natl Acad Sci USA 98:13728–13733PubMedCrossRefGoogle Scholar
- Pasmanik M, Callard G (1985) Identification and characterization of an androgen receptor in the brain of goldfish (Carassius auratus). Am Zool 25:115AGoogle Scholar
- Shu H, Zhang Y, Liu XC, Li GL, Lin HR (2006) Effects of ADSD implantation on endocrine and gonadal development in red-spotted grouper Epinephelus akaara. Curr Zool 52:316–327Google Scholar
- Wen HS, Zhang XR, Chen CF, Jin GX, Chen XY, Shen B, He F (2009) cloning and expression analysis of male half-smooth tongue solt (Cynoglossus semilaevis)’s androgen receptor gene. J Ccean Univ China 39:387–391 (In Chinesis)Google Scholar
- Wilson VS, Cardon MC, Thornton J, Korte JJ, Ankley GT, Welch J, Gray LE, Hartig PC (2004) Cloning and in vitro expression and characterization of the androgen receptor and isolation of estrogen receptor α from the fathead minnow (Pimephales promelas). Environ Sci Technol 38:6314–6321PubMedCrossRefGoogle Scholar
- Yeh S, Tsai MY, Xu Q, Mu XM, Lardy H, Huang KE, Lin H, Yeh SD, Altuwaijri S, Zhou X, Xing L, Boyce BF, Hung MC, Zhang S, Gan L, Chang C (2002) Generation and characterization of androgen receptor knockout (ARKO) mice: an in vivo model for the study of androgen functions in selective tissues. Proc Natl Acad Sci USA 99:13498–13503PubMedCrossRefGoogle Scholar
- You YL, Lin DJ (1997) The ultrastructure of the spermatozoon of the teleost, Pseudosciaena crocea (richardson). Dong Wu Xue Bao 43:119–126 (In Chinese)Google Scholar
- You YL, Lin DJ, Chen LY (2001) Spermatogenesis of teleosts, Pseudosciaena crocea. Dongwuxue Yanjiu 22:461–466 (In Chinese)Google Scholar