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Fish Physiology and Biochemistry

, Volume 39, Issue 2, pp 299–308 | Cite as

Carbon nanotube enhanced label-free immunosensor for amperometric determination of oocyte maturation-inducing hormone in fish

  • Mutsuko Hirai
  • Tadayoshi Muramatsu
  • Hitoshi Ohnuki
  • Kyoko Hibi
  • Huifeng Ren
  • Hideaki Endo
Article
  • 388 Downloads

Abstract

Maintaining high-quality fish eggs stably and efficiently is important for aquaculture. We developed a label-free immunosensor system for measuring 17,20β-dihydroxy-4-pregnen-3-one (DHP). DHP is suddenly secreted before ovulation as a maturation-inducing hormone in fish, and therefore, DHP levels are an indicator for predicting ovulation. The method is based on immunologic reactions and amperometric measurement using cyclic voltammetry (CV). For biomolecular immobilization on the surface of sensing electrode, Au electrode, we used self-assembled monolayers of thiol-containing compounds to fix anti-DHP immunoglobulin. In addition, we used a single-walled carbon nanotube to improve sensitivity. Using this electrode, we were able to determine the CV signal change caused by the antigen–antibody complex. The proposed immunosensor system showed a linear correlation (correlation coefficient: 0.9827) between the anodic peak current of the CV and the DHP level in range from 15.6 to 50,000 pg ml−1. The sensor system was then applied to monitor DHP of goldfish (Carassius auratus). Blood plasma of fish was collected every 3 h after administering a DHP inducer. In the measurement, the anodic peak current of the CV showed distinct changes depending on DHP levels in the blood plasma. A good relationship was observed between DHP levels determined by our proposed system and the conventional method (correlation coefficient: 0.9351).

Keywords

17,20β-Dihydroxy-4-pregnen-3-one Ovulation Biosensor Carbon nanotube Immunoassay Label-free Fish 

Notes

Acknowledgments

This research was supported in part by a Grant-in-Aid for Scientific Research (B) from The Ministry of Education, Culture, Sports, Science, and Technology. We wish to thank Mr. Junzo Yana (Institute of Carbon Science and Technology, Shinshu University) for their helpful discussions.

References

  1. Asahina K, Kambegawa A, Higashi T (1995) Development of a microtiter plate enzyme-linked immunosorbent assay for 17α,20β-21-trihydroxy-4-pregnen-3-one, a teleost gonadal steroid. Fish Sci 61:491–494Google Scholar
  2. Bromage N, Porter M, Randall C (2001) The environmental regulation of maturation in farmed finfish with special reference to the role of photoperiod and melatonin. Aquaculture 197:63–98CrossRefGoogle Scholar
  3. Bulukin E, Meucci V, Minunni M, Pretti C, Soldani G, Masdini M (2007) An optical immunosensor for rapid vitellogenin detection in plasma from carp (Cyprinus carpio). Talanta 72:785–790PubMedCrossRefGoogle Scholar
  4. Carmona-Osalde C, Rodriguez-Serna M, Olvera-Novoa MA, Gutierrez-Yurrita PJ (2004) Gonadal development, growth and survival of the crayfish Procambarus llamasi at three different water temperature. Aquaculture 232:305–316CrossRefGoogle Scholar
  5. Currie LA (1968) Limits for qualitative detection and quantita-tive determination: application to radiochemistry. Anal Chem 40:586–593CrossRefGoogle Scholar
  6. Duncan N, Mitchell D, Bromage N (1999) Post-smolt growth and maturation of out-of-season 0 + Atlantic salmon (Salmo salar) reared under different photoperiods. Aquaculture 177:61–71CrossRefGoogle Scholar
  7. Endo H, Igarashi M, Banba A, Ohnuki H, Ushio H, Hayashi T, Ren H, Yoshizaki G (2011) Electrode-based immunologic assay system to monitor oocyte maturation-inducing hormone in fish. Int J Environ Anal Chem 91:174–184CrossRefGoogle Scholar
  8. Endo H, Muramatsu T, Yoshizaki G, Ren H, Ohnuki H (2012) Development of a label-free immunosensor system for detecting oocyte maturation-inducing hormone in fish. Fish Sci 78:391–398CrossRefGoogle Scholar
  9. Haussmann MF, Vleck CM, Farrar ES (2007) A laboratory exercise to illustrate increased salivary cortisol in response to three stressful conditions using competitive ELISA. Adv Physiol Educ 31:110–115PubMedCrossRefGoogle Scholar
  10. Huang CH, Sedlak DL (1998) Analysis of estrogenic hormones in municipal wastewater effluent and surface water using enzyme-linked immunosorbent assay and gas chromatography/tandem mass spectrometry. Environ Toxicol Chem 20:133–139CrossRefGoogle Scholar
  11. Idler DR, Fagerlund UHM, Ronald AP (1960) Isolation of pregn-4-ene-17-alpha, 20beta-diol-3-one from the plasma of Pacific salmon (Oncorhynchus nerka). Biochem Biophys Res Commun 2:133–137CrossRefGoogle Scholar
  12. Iijima S (1991) Helical microtubules of graphitic carbon. Nature 354:56–58CrossRefGoogle Scholar
  13. Jarboe HH, Romaire RP (1995) Effects of density reduction and supplemental feeding on stunted crayfish Procambarus clarkii populations in earthen ponds. J World Aquac Soc 26:29–37CrossRefGoogle Scholar
  14. Kagawa H, Young G, Nagahama Y (1983) Changes in plasma steroid hormone levels during gonadal maturation in female goldfish Carassius auratus. Bull Jpn Soc Sci Fish 49:1783–1787CrossRefGoogle Scholar
  15. King VW, Berlimsky LD, Sullivan CV (1995) Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies. Fish Physiol Biochem 14:489–500CrossRefGoogle Scholar
  16. Kobayashi M, Adachi N (2002) The foundation of ichthyology. Kouseisha Kouseikaku Co., Ltd., Tokyo (in Japanese)Google Scholar
  17. Li ZL, Wang S, Lee NA (2004) Development of a solid-phase extraction—enzyme-linked immunosorbent assay method for the determination of estrone in water. Anal Chim Acta 503:171–177CrossRefGoogle Scholar
  18. Mason DW, Williams AF (1980) The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J 187:1–20PubMedGoogle Scholar
  19. Matsuyama M (1997) Maturation inducing hormone of fish. Radioisotopes 46:263–264CrossRefGoogle Scholar
  20. Moriwaki T, Kobayashi M, Aida K, Hanyu I (1991) Changes in plasma gonadotropin and steroid hormone levels during ovulation induced by HCG treatment in female goldfish. Bull Jpn Soc Sci Fish 57:41–43CrossRefGoogle Scholar
  21. Nagahama Y, Yamashita M (1989) Mechanisms of synthesis and action of 17α, 20β-dihydroxy-4-pregnen-3-one, a teleost maturation-inducing substance. Fish Physiol Biochem 7:193–200CrossRefGoogle Scholar
  22. Ohta K, Yamaguchi S, Yamaguchi A, Matsuyama M (2002) Biosynthesis of steroids in ovarian follicles of red seabream, Pagrus major (Sparidae, Teleostei) during final oocyte maturation and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro. Comp Biochem Physiol B 133B:45–54CrossRefGoogle Scholar
  23. Qiu LP, Wang CC, Hu P, Wu ZS, Shen GL, Yu RQ (2010) A label-free electrochemical immunoassay for IgG detection based on the electron transfer. Talanta 83:42–47PubMedCrossRefGoogle Scholar
  24. Radi AE, Muñoz-Berbel X, Lates V, Marty JL (2009) Label-free impedimetric immunosensor for sensitive detection of ochratoxin A. Biosens Bioelectron 24:1888–1892PubMedCrossRefGoogle Scholar
  25. Rivas GA, Rubianes MD, Rodriguez MC, Ferreyra NF, Luque GL, Pedano ML, Miscoria SA, Parrado C (2007) Carbon nanotubes for electrochemical biosensing. Taranta 74:291–307Google Scholar
  26. Saito R, Shinohara H (2004) Foundation and application of carbon nanotubes. Baifukan Co., Ltd., Tokyo (in Japanese)Google Scholar
  27. Su X, Chew FT, Li SFY (2000) Piezoelectric quartz crystal based label-free analysis for allergy disease. Biosens Bioelectron 15:629–639PubMedCrossRefGoogle Scholar
  28. Suzuki R, Yamaguchi M (1977) Effect of temperature on maturation of a cyprinid loach. Bull Jpn Soc Sci Fish 43:367–373CrossRefGoogle Scholar
  29. Tamura T, Itazawa Y, Oguri M, Hanyu I (1991) Fish physiology introduction. Kouseisha Kouseikaku Co., Ltd., TokyoGoogle Scholar
  30. Ulman A (1998) Thin films: self-assembled monolayers of thiols (thin films). Academic Press, New York, pp 45–65Google Scholar
  31. Vidal JC, Espuelas J, Garcia-Ruiz E, Castillo JR (2004) Amperometric cholesterol biosensors based on the electropolymerization of pyrrole and the electrocatalytic effect of Prussian-Blue layers helped with self-assembled monolayers. Talanta 64:655–664PubMedCrossRefGoogle Scholar
  32. Viswanathan S, Wu L, Huang M, Ho JA (2006) Electrochemical immunosensor for cholera toxin using liposomes and poly (3, 4-ethylenedioxythiophene)-coated carbon nanotubes. Anal Chem 78:115–1121CrossRefGoogle Scholar
  33. Wang D, Chen L (2009) Facile direct electron transfer in glucose oxidase modified electrodes. Electrochim Acta 54:4316–4320CrossRefGoogle Scholar
  34. Zhang L, Yuan R, Huang X, Chai Y, Tang D, Cao S (2005) A new label-free amperometric immunosensor for rubella vaccine. Anal Bioanal Chem 381:1036–1040PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Mutsuko Hirai
    • 1
  • Tadayoshi Muramatsu
    • 1
  • Hitoshi Ohnuki
    • 2
  • Kyoko Hibi
    • 1
  • Huifeng Ren
    • 1
  • Hideaki Endo
    • 1
  1. 1.Faculty of Marine ScienceTokyo University of Marine Science and TechnologyMinato-ku, TokyoJapan
  2. 2.Faculty of Marine TechnologyTokyo University of Marine Science and TechnologyKoto-ku, TokyoJapan

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