Fish Physiology and Biochemistry

, Volume 39, Issue 2, pp 215–220 | Cite as

Sperm production and quality in brill Scophthalmus rhombusL.: relation to circulating sex steroid levels

  • Ismael Hachero-Cruzado
  • Asunción Forniés
  • Marcelino Herrera
  • Juan Miguel Mancera
  • Gonzalo Martínez-Rodríguez
Article

Abstract

The aims of the present study were to characterize sperm quality and to quantify seasonal changes in sexual hormone (testosterone [T], 11-ketotestosterone [11-KT] and 17,20β-dihydroxypregn-4-en-3-one [17,20β-P]) levels in male brill (Scophthalmus rhombus) plasma, as well as to test a more intensive sampling strategy to establish relationships between sex steroid levels and sperm production parameters. Sperm concentration ranged from 0.5 to 3.1 × 109 spermatozoa mL−1, and changes in sperm quality parameters depending on sampling date were observed. Plasma sexual steroid levels remained high and changed in parallel during the spawning season and afterwards decreased to very low levels in summer. The analysis of annual changes of 11-KT and T ratios suggests that 11-KT can be the main circulating androgen for stimulating spermatogenesis in S. rhombus and that T could be involved in the beginning of spermatogenesis through the positive feedback on brain-pituitary-gonad axis. Finally, daily 11-KT and T levels showed similar patterns of variation in males sampled, whereas 17,20β-P amounts showed somewhat opposite trends. These differences could be related with the different role of androgens and progestin during the spermatogenesis.

Keywords

Motility Sperm concentration Scophthalmus rhombus Sex steroids 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Ismael Hachero-Cruzado
    • 1
  • Asunción Forniés
    • 2
  • Marcelino Herrera
    • 1
  • Juan Miguel Mancera
    • 3
  • Gonzalo Martínez-Rodríguez
    • 2
  1. 1.Instituto de Investigación y Formación Agraria y Pesquera de Andalucía (IFAPA)Centro Agua del PinoCartaya, HuelvaSpain
  2. 2.Instituto de Ciencias Marinas de Andalucía (ICMAN)Consejo Superior de Investigaciones Científicas (CSIC)CádizSpain
  3. 3.Departamento de Biología, Facultad de Ciencias del Mar y AmbientalesUniversidad de Cádiz (UCA)CádizSpain

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