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Fish Physiology and Biochemistry

, Volume 39, Issue 2, pp 131–142 | Cite as

Molecular cloning and expression analysis of major histocompatibility complex class IIB gene of the Whitespotted bambooshark (Chiloscyllium plagiosum)

  • Qian Ma
  • Yong-Quan Su
  • Jun Wang
  • Zhi-Meng Zhuang
  • Qi-Sheng Tang
Article

Abstract

Major histocompatibility complex (MHC) plays an important role in the immune response to antigenic peptides in vertebrates. In this study, the full length of MHC IIB cDNA was isolated from the Whitespotted bambooshark (Chiloscyllium plagiosum) by homology cloning, and the rapid amplification of cDNA ends polymerase chain reaction. As a result, the MHC IIB cDNA is 1,407 bp, which contains an open reading frame (ORF) of 831 bp encoding a protein of 276 amino acids. Furthermore, seven alleles of the complete MHC IIB ORF were detected and the variable sites were mainly located in the immunoglobulin-like (β2) region. Tissue distribution analysis showed that MHC IIB can be detected in all the ten tissues examined, with the highest expression in the spleen and gill. Challenge of C. plagiosum with the pathogenic bacteria, Vibrio harveyi, resulted in significant changes in the expression of MHC IIB mRNA in the three immune-related tissues (gill, liver and spleen). These results show that the MHC IIB plays an important role in response to bacterial infection in elasmobranches.

Keywords

Chiloscyllium plagiosum MHC IIB Sequence analysis mRNA expression Vibrio harveyi 

Notes

Acknowledgments

The research was financially supported by the National Natural Science Foundation of China (Grant No.: 40876080).

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Qian Ma
    • 1
    • 2
  • Yong-Quan Su
    • 2
  • Jun Wang
    • 2
  • Zhi-Meng Zhuang
    • 1
  • Qi-Sheng Tang
    • 1
  1. 1.Key Laboratory for Fishery Resources and Eco-environment, Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoChina
  2. 2.College of Oceanography and Environmental ScienceXiamen UniversityXiamenChina

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