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Influence of acclimation temperature on the induction of heat-shock protein 70 in the catfish Horabagrus brachysoma (Günther)

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Abstract

Every organism responds to heat stress by synthesizing a group of evolutionarily conserved proteins called the heat-shock proteins (HSPs) that, by acting as molecular chaperones, protect the cell against the aggregation of denatured proteins and play a significant role in adaptation to temperature. The present study aimed to investigate the critical thermal maxima (CTMax) and the expression of HSP70 in different tissues (gill, brain, muscle and liver) of an endemic catfish Horabagrus brachysoma acclimated at either 20 or 30°C for 30 days. To understand the HSP70 response, fish acclimated to the two temperatures were exposed to preset temperatures (26, 30, 34, 36 and 38°C for 20°C acclimated fish and 32, 34, 36, 38 and 40°C for 30°C acclimated fish) for 2 h, followed by 1 h recovery at their respective acclimation temperatures. The HSP70 levels in the gill, brain, muscle and liver tissues were determined by Western blotting of one-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis. A significant (P < 0.05) increase in the CTMax values was observed for fish acclimated at 30°C (41.86 ± 0.39°C) than those acclimated at 20°C (39.13 ± 0.18°C). HSP70 was detected in all the tissues with the highest level in the liver followed by intermediate levels in muscle and brain, and lowest level in gill tissue, irrespective of the acclimation temperatures (20 or 30°C). The HSP70 levels were significantly higher (P < 0.05) in the tissues of fish acclimated at 30°C than those acclimated at 20°C. The mean induction temperature of HSP70 in all the tissues of fish acclimated at either 20 or 30°C was 30 and 34°C, respectively. The optimum temperature for HSP70 induction in all the tissues of fish acclimated at 20°C was 36°C, whereas for fish acclimated at 30°C was 36°C for gill and 38°C for brain, muscle and liver. Decreased levels of HSP70 were noted in all the tissues of fish when exposed to temperatures that exceeded the optimum temperatures for HSP70 inductions. Overall results indicated that acclimation temperature influences both temperature tolerance and induction of HSP70 in H. brachysoma.

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Acknowledgments

The first author was senior research fellow in the thermal ecology project funded by the Board of Research in Nuclear Sciences (BRNS). The authors acknowledge the financial support from BRNS, Department of Atomic Energy, Government of India (BRNS Sanction No. 99/36/22/BRNS, Grant No. 089).

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Authors and Affiliations

  1. Division of Fish Nutrition Biochemistry and Physiology, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    Rishikesh S. Dalvi & Asim K. Pal

  2. Department of Zoology, Maharshi Dayanand College, Parel, Mumbai, 400012, India

    Lalchand R. Tiwari

  3. Laboratory of Aquaculture Artemia Reference Centre, Ghent University, Rozier 44, 9000, Ghent, Belgium

    Kartik Baruah

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  1. Rishikesh S. Dalvi
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Correspondence to Rishikesh S. Dalvi.

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Dalvi, R.S., Pal, A.K., Tiwari, L.R. et al. Influence of acclimation temperature on the induction of heat-shock protein 70 in the catfish Horabagrus brachysoma (Günther). Fish Physiol Biochem 38, 919–927 (2012). https://doi.org/10.1007/s10695-011-9578-9

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