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Fish Physiology and Biochemistry

, Volume 38, Issue 3, pp 635–643 | Cite as

The protective role of ascorbic acid (vitamin C) against chlorpyrifos-induced oxidative stress in Oreochromis niloticus

  • Ferbal ÖzkanEmail author
  • Suna Gül Gündüz
  • Mehmet Berköz
  • Arzu Özlüer Hunt
  • Serap Yalın
Article

Abstract

Ability of ascorbic acid (vitamin C) to attenuate oxidative damage was evaluated in liver and brain tissues of Oreochromis niloticus (O. niloticus) experimentally exposed to sublethal concentrations of chlorpyrifos (CPF). O. niloticus was exposed to sublethal concentrations of CPF at 12 μg/L (CPF1) and 24 μg/L (CPF2) for 96 h. The fish of vitamin C (Vit C) and CPF2 + Vit C groups were fed with Vit C supplemented diet (200 mg Vit C/100 g feed). A significant increase in thiobarbituric acid-reactive substances (TBARS) level (P < 0.05) was observed in brain of CPF-exposed fish although liver TBARS level was not changed compared to control group. This result showed that lipid peroxidation (LPO) was elevated in brain of fish exposed to CPF. Glutathione peroxidase (GSH-Px) activity in liver and brain tissues was significantly elevated (P < 0.05) by exposure to CPF1 and CPF2. Catalase (CAT) activity was significantly increased (P < 0.05) in liver but decreased in brain of treated fish by CPF2 concentration. Superoxide dismutase (SOD) activity was decreased in liver, but increased in brain by exposure to CPF1 and CPF2 concentrations. Levels of TBARS were increased in brain of CPF-treated animals, but tended to decrease by the effect of Vit C. Vit C treatment for CPF-intoxicated animals normalized the otherwise raised activities of GSH-Px, CAT, and SOD within normal limits. The results clearly indicate that exposure to CPF caused a dose-dependent increase in oxidative stress brain and to a lesser extend in liver of fish and the ability of Vit C to attenuate CPF-induced oxidative damage.

Keywords

Chlorpyrifos Oreochromis niloticus Oxidative stress Antioxidant Vitamin C 

References

  1. Aebi H (1974) Catalase. In: Bergmayer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 671–684Google Scholar
  2. Bagnyukova TB, Vasylkiva OY, Storeyb KB, Lushchak VI (2005) Catalase inhibition by amino triazole induces oxidative stress in goldfish brain. Brain Res 1052:180–186PubMedCrossRefGoogle Scholar
  3. Ballesteros ML, Wunderlin DA, Bistoni MA (2009) Oxidative stres responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotox Environ Safe 72:199–205CrossRefGoogle Scholar
  4. Bebe FN, Panemangalore M (2003) Exposure to low doses of endosulfan and chlorpyriphos modifies endogenous antioxidants in tissues of rats. J Environ Sci Heal 38:349–363CrossRefGoogle Scholar
  5. Craig PM, Chris M, Wood CM, McClelland GB (2007) Oxidative stress response and gene expression with acute copper exposure in zebrafish (Danio rerio). Am J Physiol Regul Integr Comp Physiol 293:1882–1892CrossRefGoogle Scholar
  6. Datta M, Kaviraj A (2003) Ascorbic acid supplementation of diet for reduction of deltamethrin induced stress in freshwater catfish Clarias gariepinus. Chemosphere 53:883–888PubMedCrossRefGoogle Scholar
  7. Dimitrova MST, Tsinova V, Velcheva V (1994) Combined effect of zinc and lead on the hepatic superoxide dismutase–catalase system in carp (Cyprinus carpio). Comp Biochem Phys 108:43–46Google Scholar
  8. El-Gendy SK, Aly NM, Mahmoud FH, Kenawy A, El-Sebae AKH (2010) The rol of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food Chem Toxicol 48:215–221PubMedCrossRefGoogle Scholar
  9. Fetoui H, Garoui EM, Makni-ayadi F, Zeghal N (2008) Oxidative stress induced by lambda-cyhalothrin (LTC) in rat erythrocytes and brain: attenuation by vitamin C. Environ Toxicol Phar 26:225–231CrossRefGoogle Scholar
  10. Galloway T, Handy R (2003) Immunotoxicity of organophosphorous pesticides. Ecotoxicol 12:345–363CrossRefGoogle Scholar
  11. Guha D, Dutta K, Das M (1993) Vitamin C as antitoxic factor in DDT induced haematotoxicity in Clarias batrachus. Proc Zool Soc Calcutta 46:11–15Google Scholar
  12. Gupta RC (2004) Brain regional heterogeneity and toxicological mechanisms of organophosphates and carbamates. Toxicol Mech and Methods 14:103–143CrossRefGoogle Scholar
  13. Hai DQ, Varga SI, Matkovics B (1997) Organophosphate effects on antioxidant system of carp (Cyprinus carpio) and catfish (Ictalurus nebulosus). Comp Biochem Phys C 117:83–88Google Scholar
  14. Huculeci R, Dinu D, Staicu AC, Munteanu MC, Costache M, Dinischiotu A (2008) Malathion-induced alteration of the antioxidant defence system in kidney, gill, and intestine of Carassius auratus gibelio. Environ Toxicol 24:523–530CrossRefGoogle Scholar
  15. Hussain AL (2008) Subchronic intoxication with chlorfenvinphos, an organophosphate insecticide, affects rat brain antioxidative enzymes and glutathione level. Food Chem Toxicol 46:82–86CrossRefGoogle Scholar
  16. Jocely PC (1970) The enzymic oxidation of glutathione in rat liver homogenates. Biochem J 117:947–949Google Scholar
  17. Kavitha P, Rao V (2008) Toxic effects of chlorpyrifos on antioxidant enzymes and target enzyme acetylcholinesterase interaction in mosquito fish, Gambusia affinis. Environ Toxicol Phar 26:192–198CrossRefGoogle Scholar
  18. Korkmaz N, Cengiz EI, Unlu E, Uysal E, Yanar M (2009) Cypermethrin-induced histopathological and biochemical changes in Nile tilapia (Oreochromis niloticus), and the protective and recuperative effect of ascorbic acid. Environ Toxicol Phar 28:198–205CrossRefGoogle Scholar
  19. Kwonq TC (2002) Organophosphate pesticides: biochemistry and clinical toxicology. Ther Drug Monit 24:144–149CrossRefGoogle Scholar
  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin Phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  21. Lushchak OV, Olha I, Kubrak OI, Storey JM, Storey KB, Lushchak VI (2009) Low toxic herbicide Roundup induces mild oxidative stress in goldfish tissues. Chemosphere 76:932–937PubMedCrossRefGoogle Scholar
  22. Mehta A, Verma RS, Srivastava N (2005) Chlorpyrifos induced alterations in rat brain ATPases, acetylcholinesterase and lipid peroxidation. Indian J Biochem Bio 42:54–58Google Scholar
  23. Mehta A, Verma RS, Srivastava N (2009) Chlorpyrifos induced alterations in the levels of hydrogen peroxide, nitrate and nitrite in rat brain and liver. Pestic Biochem and Phys 94:55–59CrossRefGoogle Scholar
  24. Monteiro DA, Almeida JA, Rantin FT, Kalinin AL (2006) Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Com Biochem Phys C 143:141–149CrossRefGoogle Scholar
  25. Ortuno J, Esteban MA, Meseguer J (2003) The effect of dietary intake of vitamins C and E on the stress response of gilthead seabream (Sparus aurata L.). Fish Shellfish Immun 14:145–156CrossRefGoogle Scholar
  26. Oruç EO (2010) Oxidative stress, steroid hormone concentrations and acetylcholinesterase activity in Oreochromis niloticus exposed to chlorpyrifos. Pestic Biochem Phys 96:160–166CrossRefGoogle Scholar
  27. Oruç EO, Usta D (2007) Evaluation of oxidative stres responses and neurotoxicity potential of diazinonin different tissues of Cyprinus carpio. Environ Toxicol Phar 23:48–55CrossRefGoogle Scholar
  28. Padayatty SJ, Katz A, Wang Y et al (2003) Vitamin C as an antioxidant: evaluation of its role in disease prevention. J Am Coll Nutr 22:18–35PubMedGoogle Scholar
  29. Palace VP, Dick TA, Brown SB, Baron CL, Klaverkamp JF (1996) Oxidative stress in Lake Sturgeon (Acipenser fulvescens) orally exposed to 2,3,7,8-tetrachlorodibenzofuran. Aquat Toxicol 35:79–92CrossRefGoogle Scholar
  30. Pandey S, Parvez S, Sayeed I, Haquer R, Bin-Hafeez B, Raisuddin S (2003) Biomarkers of oxidative stress: a comparative study of river Yamunafish Wallagoattu (Bl. Schn). Sci Total Environ 309:105–115PubMedCrossRefGoogle Scholar
  31. Peixoto F, Alves-Fernandes D, Santos D, Fontainhas-Fernandes A (2006) Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromis niloticus. Pestic Biochem Phys 85:91–96CrossRefGoogle Scholar
  32. Song SB, Xu Y, Zhou BS (2006) Effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (Cyprinus carpio). Chemosphere 65:699–706PubMedCrossRefGoogle Scholar
  33. Sun Y, Oberley LW, Ying L (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500PubMedGoogle Scholar
  34. Thomaz JM, Martins ND, Monteiro DA, Rantin FT, Kalinin AL (2009) Cardio-respiratory function and oxidative stres biomarkers in Nile tilapia exposed to the organophosphate insecticide trichlorfon (NEGUVONs). Ecotox Environ Safe 72:1413–1424CrossRefGoogle Scholar
  35. Valavanidis A, Vlahogianni T, Dassenakis M, Scoullos M (2006) Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotox Environ Safe 64:178–189CrossRefGoogle Scholar
  36. Van der Oost R, Beyer J, Vermeulen NP (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Phar 13:57–149CrossRefGoogle Scholar
  37. Varo I, Serrano R, Pitarch E, Amat F, Lopez FJ, Navarro JC (2002) Bioaccumulation of chlorpyrifos through an experimental food chain: study of protein HSP70 as biomarker of sublethal stress in fish. Arch Environ Con Tox 42:229–235CrossRefGoogle Scholar
  38. Verma RS, Mehta A, Srivastava N (2007) In vivo chlorpyrifos induced oxidative stress: attenuation by antioxidant vitamins. Pestic Biochem Phys 88:191–196CrossRefGoogle Scholar
  39. Verma RS, Mehta A, Srivastava N (2009) Comparative studies on chlorpyrifos and methyl parathion induced oxidative stress in different parts of rat brain: attenuation by antioxidant vitamins. Pestic Biochem Phys 95:152–158CrossRefGoogle Scholar
  40. Yagi K (1998) Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods Mol Biol 108:107–110PubMedGoogle Scholar
  41. Zhang J, Shen H, Wang X, Wu J, Xue Y (2004) Effects of chronic exposure of 2, 4- dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 55:167–174PubMedCrossRefGoogle Scholar
  42. Zhang X, Yang F, Zhang X, Xu Y, Liao T, Song S, Wang J (2008) Induction of hepatic enzymes and oxidative stress in Chinese rare minnow (Gobiocypris rarus) exposed to waterborne hexabromocyclododecane (HBCDD). Aquat Toxicol 86:4–11PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Ferbal Özkan
    • 1
    Email author
  • Suna Gül Gündüz
    • 1
  • Mehmet Berköz
    • 2
  • Arzu Özlüer Hunt
    • 1
  • Serap Yalın
    • 2
  1. 1.Department of Basic Sciences, Faculty of FisheriesMersin UniversityMersinTurkey
  2. 2.Faculty of PharmacyMersin UniversityMersinTurkey

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