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Effect of substituting live feed with formulated feed on the reproductive performance and fry survival of Siamese fighting fish, Betta splendens (Regan, 1910)

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Abstract

This study aimed to elucidate the effect of partial or complete replacement of live feed (LF) (Tubifex) with formulated feed (FF) on the reproductive performance of Betta splendens. Three hundred B. splendens fry (average weight 0.19 ± 0.01 g) were equally distributed into five different groups, each with three replicates. They were fed for 105 days with following different diets: control (C)—100% LF; T1—75% LF, 25% FF; T2—50% LF, 50% FF; T3—25% LF, 75% FF, and T4—100% FF. Results showed that the average number of hatched larvae (654 ± 101) and fry survival after 2 weeks of rearing (428 ± 70), after completion of three spawning, were recorded highest in the control group, which was, however, not significantly different from the T1, T2, and T3 groups. At the end of the feeding trial, the highest hatching percentage (90.3%) was registered in the T2 group, which was not significantly different from the control and T1 groups. The T2 group also recorded highest fry survival (65.54%) after completion of three spawning, which was not significantly (P < 0.05) different from the T1 and T3 groups. Control diet contained higher saturated fatty acid (63.23%) than formulated diet (29.80%). In the whole-body tissue, highest level of EPA (0.42%) and DHA (3.13%) were found in the T4 group followed by T3 group. The DHA/EPA ration was recorded highest in the T2 group (10.96%), which did not differ significantly from the T1 and T3 groups. Significant positive correlation was observed between saturated fatty acid levels in fish whole-body tissue and number of hatched larvae (Y = 30.81 × −825.3, R 2 = 0.968) and fry survival after 2 weeks of rearing (Y = 21.38 × −580.9, R 2 = 0.967). Considering all these factors, it can be concluded that the live feed Tubifex can be replaced up to 50% without any adverse effect on the reproductive performance and fry survival of B. splendens.

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Acknowledgment

The first author is thankful to the Director, Central Institute of Fisheries Education (ICAR), Mumbai, India, for providing facilities and the Vice-chancellor, Central Agricultural University, Imphal, India, for granting permission to carry out the research.

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Authors and Affiliations

  1. Department of Aquaculture, College of Fisheries, CAU, Lembucherra, Tripura, 799210, India

    Sagar C. Mandal

  2. Division of Aquaculture, Central Institute of Fisheries Education, Mumbai, 400061, India

    Mahinder P. S. Kohli, Pronob Das & Soibam K. Singh

  3. Division of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Mumbai, 400061, India

    Sukham Munilkumar

  4. Division of Fisheries Science, Central Agricultural Research Institute, ICAR, Port Blair, 744101, India

    Kamal Sarma

  5. Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, 9000, Ghent, Belgium

    Kartik Baruah

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  1. Sagar C. Mandal
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  2. Mahinder P. S. Kohli
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  3. Pronob Das
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  4. Soibam K. Singh
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  5. Sukham Munilkumar
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  6. Kamal Sarma
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  7. Kartik Baruah
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Correspondence to Kartik Baruah.

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Mandal, S.C., Kohli, M.P.S., Das, P. et al. Effect of substituting live feed with formulated feed on the reproductive performance and fry survival of Siamese fighting fish, Betta splendens (Regan, 1910). Fish Physiol Biochem 38, 573–584 (2012). https://doi.org/10.1007/s10695-011-9539-3

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  • DOI: https://doi.org/10.1007/s10695-011-9539-3

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