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High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock

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Abstract

The amelioration effect of high dietary protein against stress was evaluated in Labeo rohita fingerlings, exposed to heat shock. Two hundred and forty fingerlings (6.57 ± 0.04 g, average weight ± SE) were randomly distributed into 4 treatment groups, each with 4 replicates was fed with either of four diets containing different levels of protein (20, 30, 40 or 45%). Water temperatures of all the treatments were within the range of 25.5–26.5°C throughout the experimental period of 30 days. After 30 days of feeding, fish were given heat shock by exposing to 38°C for 2 h. Heat shock significantly decreased (P < 0.05) liver glycogen content in treatment groups fed with 20 and 30% dietary protein, whereas unaffected in the 40 and 45% protein-fed groups. Heat shock significantly increased (P < 0.05) serum glucose and cortisol level in all the treatments. The 40 and 45% dietary protein–fed groups registered significantly higher survival (%) after the heat shock compared with their lower-protein counterparts. Heat shock increased the glycolytic, gluconeogenic, protein metabolic and antioxidative enzymes to cope up with thermal stress. Our results indicate that high-protein diet (≥40%) combats the stress due to heat shock in Labeo rohita.

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Acknowledgments

The first author is thankful to the Director, Central Institute of Fisheries Education for providing financial assistance in the form of an Institutional fellowship during the research period.

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  1. Krishi Vigyan Kendra, Rajendra Agricultural University, Madhopur, Bihar, 845454, India

    Shivendra Kumar

  2. Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    Shivendra Kumar, N. P. Sahu, A. K. Pal & Saravanan Subramanian

  3. College of Fisheries, Lembuchera, Central Agricultural University, Imphal, Manipur, India

    Himanshu Priyadarshi

  4. Department of Aquaculture System and Animal Nutrition in the Tropics and Subtropics (480b), University of Hohenheim, 70599, Stuttgart, Germany

    Vikas Kumar

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Kumar, S., Sahu, N.P., Pal, A.K. et al. High dietary protein combats the stress of Labeo rohita fingerlings exposed to heat shock. Fish Physiol Biochem 37, 1005–1019 (2011). https://doi.org/10.1007/s10695-011-9504-1

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