Abstract
The use of immunostimulants as an alternative to the drugs, chemicals and antibiotics currently being used to control fish diseases in fish culture is attracting the attention of many researchers. In this context, many have focused on the use of medicinal plant products as potential therapeutic measures for modulating the immune response and, specifically, on the use of herbs to prevent and control fish diseases. Medicinal plants (plant remedies) are a deeply rooted component of the cultural heritage of many people from diverse cultures and countries and are, as such, closely linked to the maintenance of good health. The aim of this paper is to review research currently being carried out on the herbs and herbal extracts that have been shown to modulate the immune system of fish. Special attention is given to the use of Chinese and Indian herbs.
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References
Agarwal SS, Singh VK (1999) Immunomodulators studies on Indian medicinal plants and synthetic Part1: medicinal plants. Proc Indian Natl Sci Acad 65:179–204
Ahmad I, Mehmood Z, Mohammed F (1998) Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 62:183–193. doi:10.1016/S0378-8741(98)00055-5
Anderson DP (1992) Immunostimulants, adjuvants and vaccine carriers in fish: application to aquaculture. In: Faisal M, Hetrick FM (eds) Annual review of fish diseases. Pergamon Press, New York, pp 281–307
Ardó L, Yin G, Xu P, Váradi L, Szigeti G, Jeney Z, Jeney G (2008) Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture 275:26–33. doi:10.1016/j.aquaculture.2007.12.022
Auro de Ocampo A, Jimenez EM (1993) Herbal medicines in the treatment of fish diseases in Mexico. Veterinaria (Mex) 24:291–295
Balasubramani SP, Michael RD (2002) Immunomodulation by the fruit extract of Indian Gooseberry, Phyllanthus emblica (Linn). in Oreochromis mossambicus (Peters). MSc thesis. The American College, Madurai
Bhattacharya A, Ghosal S (2000) Antioxidant activity of tannoid principles of Emblica officinalis (amla) in chronic stress induced changes in rat brain. Indian J Exp Biol 38:877–880
Cao LZ, Lin ZB (2003) Regulatory effect of Ganoderma lucidum polysaccharides on cytotoxic T-lymphocytes induced by dendritic cells in vitro. Acta Pharmacol Sin 24:312–326
Cao G, Sun X, Sheng J, Zhao H, Xie J, Luo L, Zhang J, Li J (1999) The effects of ginseng and Radix Astragali on function of alveolus macrophage in pneumoconiesis rats. Chin Public Health 15:1064
Cao L, Ding W, Zhang L, Jeney G, Yin G (2008) Effect of the activation of immunological cells in the common carp after stimulation by Lentinan and Astragalus polysaccharides. J Anhui Agric Univ 35:219–223
Castro R, Lamas J, Morais P, SanmartÃn ML, Orallo F, Leiro J (2008) Resveratrol modulates innate and inflammatory responses in fish leucocytes. Vet Immunol Immunopathol 126:9–19
Chansue N, Ponpornpisit A, Endo M, Sakai M, Satoshi Y (2000) Improved immunity of tilapia Oreochromis niloticus by C-UP III, a herb medicine. Fish Pathol 35:89–90
Chen X, Wu Z, Yin J, Li L (2003) Effects of four species of herbs on immune function of Carassius auratus gibelio. J Fish Sci China 10:36–40
Chopra RN, Chopra IC, Handa KL, Kapur LD (1956) Chopra’s indigenous drugs of India. UN Dhur and Sons, Calcutta
Dahanukar SA, Thatte UM (1997) Current status of ayurveda. Phytomedicine 4:297–306
Devasagayam TPA, Sainis KB (2002) Immune system and antioxidants, especially those derived from Indian medicinal plants. Indian J Exp Biol 40:639–655
Devi PU, Ganasoundari A (1995) Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum. Indian J Exp Biol 33:205–208
Direkbusarakom S, Herunsalee A, Yoshimizu M, Ezura Y (1996) Antiviral activity of several Thai traditional herb extracts against fish pathogenic viruses. Fish Pathol 31:209–213
Divyagnaneswari M, Christybapita D, Michael RD (2007) Enhancement of nonspecific immunity and disease resistance in Oreochromis mossambicus by Solanum trilobatum leaf fractions. Fish Shellfish Immunol 23:249–259. doi:10.1016/j.fsi.2006.09.015
Dügenci SK, Arda N, Candan A (2003) Some medicinal plants as immunostimulant for fish. J Ethnopharmacol 88:99–106. doi:10.1016/S0378-8741(03)00182-X
Dutta BK, Rahman I, Das TK (1998) Antifungal activity of Indian plant extracts. Mycoses 41:535–536
Edahiro T, Hamoguchi M, Kusda R (1990) Effect of glycryrrhizine against streptococcal infection of young yellowtail. Seriola quinqueradiata (in Japanese). Suisanzoshoku 38:239–243
Engstadt R, Robertsen B (1993) Recognition of yeast wall glucan by Atlantic salmon (Salmo salar). Dev Comp Immunol 17:319–330. doi:10.1016/0145-305X(93)90004-A
Evans M (1994) A guide to herbal remedies. Orient paperbacks, India
Galeotti M (1998) Some aspects of the application of immunostimulants and a critical review of methods for their evaluation. J Appl Ichthyol 14:189–199. doi:10.1111/j.1439-0426.1998.tb00641.x
Gao X, Wu W (1994) The effect of Dang-Gui (Angelica Sinensis) and ferulic acid on mice immune system. Chin J Biochem Pharmaceutics 15:107–110
Goetz FW, Iliev DB, McCauley LAR, Liarte CQ, Tort LB, Planas JV, MacKenzie S (2004) Analysis of genes isolated from lipopolysaccharide-stimulated rainbow trout (Oncorhynchus mykiss) macrophages. Mol Immunol 41:1199–1210. doi:10.1016/j.molimm.2004.06.005
Govindachari TR (1992) Chemical and biological investigations on Azadirachta indica (the neem tree). Curr Sci 63:117
Govindan S, Viswanathan S, Vijayasekaran V, Alagappan R (2004) Further studies on the clinical efficacy of Solanum xanthocarpum and Solanum trilobatum in bronchial asthma. Phytother Res 18:805–809. doi:10.1002/ptr.1555
Hemapriya VS (1997) Immunostimulatory effect of leaf extracts of few medicinal plants in Oreochromis mossambicus (Peters), MSc thesis. The American College, Madurai
Horvath CR, Martos PA, Saxena PK (2005) Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant Huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection. J Chromatogr A 1062:199–207. doi:10.1016/j.chroma.2004.11.030
Ihantola-Vormisto A, Summanen J, Kankaanranta H, Vuorela H, Asmawi ZM, Moilanen E (1997) Anti inflammatory activity of extracts from leaves of Phyllanthus emblica. Planta Med 63:518–524. doi:10.1055/s-2006-957754
Jeney G, Anderson DP (1993) Enhanced immune response and protection in rainbow trout to Aeromonas salmonicida bacterin following prior immersion in immunostimulants. Fish Shellfish Immunol 3:51–58. doi:10.1006/fsim.1993.1005
Jian J, Wu Z (2003) Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture 218:1–9. doi:10.1016/S0044-8486(02)00192-8
Jian J, Wu Z (2004) Influences of traditional Chinese medicine on non-specific immunity of Jian carp (Cyprinus carpio var. Jian). Fish Shellfish Immunol 16:19–185. doi:10.1016/S1050-4648(03)00062-7
Khanna D, Sethi G, Ahn KS, Pandey MK, Kunnumakkara AB, Sung B, Aggarwal A, Aggarwal BB (2007) Natural products as a gold mine for arthritis treatment. Curr Opin Pharmacol 7:344–351. doi:10.1016/j.coph.2007.03.002
Khopde SM, Priyadarsini KI, Mohan H, Gowandi VB, Satav JG, Yakhmi JV, Banavaliker MM, Biyani MK, Mittal JP (2001) Characterizing the antioxidant activity of amla (Phyllanthus emblica) extract. Curr Sci 81:185–190
Kim H, Moor E, Li E, Kim K, Nam S, Chung C (1999) The nitric oxide-producing activities of Scutellaria baicalensis. Toxicity 135:109–117. doi:10.1016/S0300-483X(99)00047-5
Kokate CE, Purohit AP, Gokhale SB (1999) Pharmacognosy. Nirali Prakashan, Pune
Kong X, Hu Y, Song D (2003) Research progress of immunopharmacology of Astragalus polysaccharide. J Chin Vet 3:34–37 China
Lee JH, Ko WS, Kim YH, Kang HS, Kim HD, Choi BT (2001) Anti-inflammatory effect of the aqueous extract from lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaB-alpha degradation in rat liver. Int J Mol Med 7:79–83
Lee YS, Han OK, Park CW, Yang CH, Jeon TW, Yoo WK, Kim SH, Kim HJ (2005) Pro-inflammatory gene expression and nitric oxide regulation of aqueous extracted Astragali radix in RAW 264.7 macrophage cells. J Ethnopharmacol 100:289–294. doi:10.1016/j.jep.2005.03.009
Li Y, Lovell T (1985) Elevated levels of dietary ascorbic acid increased immune response in channel cat fish. J Nutr 115:123–131
Lin ZB, Zhang HN (2004) Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms. Acta Pharmacol Sin 25:1387–1395
Liu HB (2002) Research status of Chinese herbal immunostimulants and their application in aquaculture. J Fish 15:91–94
Liu C, Tsai W, Lin Y, Liao J, Chen C, Kuo Y (2004) The extracts from Nelumbo nucifera suppress cell cycle progression, cytokine genes expression, and cell proliferation in human peripheral blood mononuclear cells. Life Sci 75:699–716. doi:10.1016/j.lfs.2004.01.019
Logambal SM, Michael RD (2000) Immunostimulatory effect of azadirachtin in Oreochromis mossambicus (Peters). Indian J Exp Biol 38:1092–1096
Logambal SM, Michael RD (2001) Azadirachtin—an immunostimulant for Oreochromis mossambicus. J Aquacult Trop 16:339–347
Logambal SM, Venkalalakshmi S, Michael RD (2000) Immunostimulatory effect of leaf extract of Ocimum sanctum Linn. in Oreochromis mossambicus (Peters). Hydrobiologia 430:113–120. doi:10.1023/A:1004029332114
Magnadóttir B (2006) Innate immunity of fish (overview). Fish Shellfish Immunol 20:137–151. doi:10.1016/j.fsi.2004.09.006
Misra CK, Das BK, Mukherjee SC, Meher PK (2006) The immunomodulatory effects of tuftsin on the non-specific immune system of Indian Major car, Labeo rohita. Fish Shellfish Immunol 20:728–738. doi:10.1016/j.fsi.2005.09.004
Mohan PV, Devi KS (1997) Effect of Sobatum on tumour development and chemically induced carcinogenesis. Cancer Lett 112:219–223. doi:10.1016/S0304-3835(96)04574-0
Mohan PV, Rao JM, Kutty MAS, Devi KS (1998) Cytotoxicity of extracts of Solanum trilobatum and anticarcinogenic activity of Sobatum. Biomedicine 18:106–111
Peddie S, Secombes CJ (2003) The immunostimulatory effects of Chevimmun in the rainbow trout (Oncorhynchus mykiss). Bull Eur Assoc Fish Pathol 23:48–51
Ponpornpisit A, Endo M, Murata H (2001) Prophylactic effects of chemicals and immunostimulants in experimental Tetrahymena infection of guppy. Fish Pathol 36:1–6
Qiu Y, Cui B (2008) Effects of four polysaccharides on antibody titer and T-lymphocyte in vaccinated chicken. J Nanjing Agric Univ 31(1):77–78
Raa J (2000) The use of immune-stimulants in fish and shellfish feeds. In: Cruz-Suarez LE, Ricque-Marie D, Tapia-Salazar M, Olvera-Novoa MA, Civera-Cerecedo R (eds) Advance en Nutricion Acuicola V. Memorias del V Simposium Internacional de Nutrcion Acouicola. Merida, Yucatan, pp 47–56
Raa J, Roerstadt G, Engstadt R, Robertsen B (1992) The use of immunostimulants to increase resistance of aquatic organisms to microbial infections. In: Shariff M, Subasinghe RP, Arthur JR (eds) Diseases in Asian aquaculture. I. Proc 1st Symp Dis Asian Aquacult. Manila, Philippines, pp 39–50
Ray A, Banerjee BD, Sen P (1996) Modulation of humoral and cell mediated immune response by Azadirachta indica (Neem) in mice. Indian J Exp Biol 34:698–701
Sadekar RD, Kolte AY, Barmase BS, Desai VF (1998) Immunopotentiating effects of Azadirachta indica (Neem) dry leaves powder in broilers, naturally infected with IBD virus. Indian J Exp Biol 36:1151–1153
Sakai M (1999) Current research status of fish immunostimulant. Aquaculture 172:63–92. doi:10.1016/S0044-8486(98)00436-0
Secombes CJ, Fletcher TC (1992) The role of phagocytes in the protective mechanisms of fish. Annu Rev Fish Dis 2:53–71. doi:10.1016/0959-8030(92)90056-4
Sen P, Medirapta P, Ray A, Puri S (1996) An experimental evaluation of Azadirachta indica in normal and stressed rats and adaptogenic effect. In: Chary MS, Singh PR, Kraus W, Saxena RC (eds) Abstracts World Neem Conf. Oxford and IBH Publ, London
Shahjahan M, Sabitha KE, Jainu M, Shyamala Devi CS (2004) Effect of Solanum trilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian J Med Res 120:194–198
Shan BE, Yoshida Y, Sugiura T, Yamashita U (1999) Stimulating activity of Chinese medicinal herbs on human lymphocytes in vitro. Int J Immunopharmacol 21:149–159. doi:10.1016/S0192-0561(98)00074-5
Shan J, Wang S, Liu D, Hu Z (2000) Progress of chemical and pharmacological study of Astragalus polysaccharide. Acta Univ Traditionis Med Sin Pharmacol Shanghai 14:61–65
Shao B, Xu W, Dai H, Tu P, Li Z, Gao X (2004) A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochem Biophys Res Commun 320:1103–1111. doi:10.1016/j.bbrc.2004.06.065
Song QH, Kobayashi T, Xiu LM, Tie H, Cyong JC (2000) Effects of Astragali root and Hedysari root on the murine B and T cell differentiation. J Ethnopharmacol 73(1–2):111–119. doi:10.1016/S0378-8741(00)00273-7
Tan BKH, Vanitha J (2004) Immunomodulatory and antimicrobial effect of some traditional Chinese medicinal herbs. Curr Med Chem 11:1423–1430
Upadhyay SN, Dawan S, Garg S, Talwar GP (1992) Immunomodulatory antifertility effect of neem (Azadirachta indica) oil. Int J Immunopharmacol 14:1187–1193. doi:10.1016/0192-0561(92)90054-O
Venkatalakshmi S, Michael RD (2001) Immunostimulation by leaf extract of Ocimum sanctum L. in Oreochromis mossambicus (Peters). J. Aquat Trop 16:1–10
Wang SY, Hsu ML, Hsu HC, Tzeng CH, Lee SS, Siao MS, Ho CK (1997) The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int J Cancer 70:699–705. doi:10.1002/(SICI)1097-0215(19970317)70:6<699::AID-IJC12>3.0.CO;2-5
Wang R, Li D, Bourne S (1999) Can 2000 years of herbal medicine history help us to solve problems in the year 2000? In: Biotechnology in the feed industry. Proc Alltech’s 14th Ann Symp. University Press, Nottingham, pp 273–291
Wu W, Ye J, Lu Q, Wu H, Pan Q (1998) Studies on Gynostemma pentaphyllum used as fish feed additives. J Shanghai Fish Univ 7:367–370
Wu H, Luo J, Yin Y, Wei Q (2004) Effects of chlorogenic acid, an active compound activating calcineurin, purified from Flos Lonicerae on macrophage. Acta Pharmacol Sin 12:1685–1689
Yin G, Wiegertjes G, Li Y, Schrama J, Verreth J, Xu P, Zhou H (2004) Effect of Astragalus radix on proliferation and nitric oxide production of head kidney macrophages in Cyprinus carpio: an in vitro study. J Fish China 28(6):628–632
Yin G, Jeney G, Rácz T, Pao X, Jeney Z (2006) Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus. Aquaculture 253:39–47. doi:10.1016/j.aquaculture.2005.06.038
Yin G, Ardó L, Thompson KD, Adams A, Jeney Z, Jeney G (2009) Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila. Fish Shellfish Immunol 26:140–145. doi:10.1016/j.fsi.2008.08.015
You YH, Lin ZB (2002) Protective effects of Ganoderma lucidum polysaccharides peptide on injury of macrophages induced by reactive oxygen species. Acta Pharmacol Sin 23:787–791
Yuan C, Li D, Chen W, Sun F, Wu G, Gong Y, Tang J, Shen M, Han X (2007) Administration of a herbal immunoregulation mixture enhances some immune parameters in carp (Cyprinus carpuio). Fish Physiol Biochem 33:93–101. doi:10.1007/s10695-006-9120-7
Yuan C, Pan X, Gong Y, Xia A, Wu G, Tang J, Han X (2008) Effects of Astragalus polysaccharides (APS) on the expression of immune response genes in head kidney, gill and spleen of the common carp, Cyprinus carpio L. Int Immunopharmacol 8:51–58. doi:10.1016/j.intimp.2007.10.009
Zhang J, Tang Q, Zimmerman-Kordman M, Reutter W, Fan H (2002) Activation of B lymphocytes by GLIS, a bioactive proteglucan from Ganoderma lucidum. Life Sci 71:623–638. doi:10.1016/S0024-3205(02)01690-9
Zhou X, Niu C, Sun R (2003) The effects of Astragalus radix on immunity and anti-stress ability in soft-shelled turtles (Trionyx Sinensis). Acta Hydrobiol Sin 27:110–112
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Galina, J., Yin, G., Ardó, L. et al. The use of immunostimulating herbs in fish. An overview of research. Fish Physiol Biochem 35, 669–676 (2009). https://doi.org/10.1007/s10695-009-9304-z
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DOI: https://doi.org/10.1007/s10695-009-9304-z
Keywords
- Aquaculture
- Chinese herbs
- Fish culture
- Immunostimulants
- Indian herbs
- Innate immunity
- Medicinal herbs