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Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps

Fish Physiology and Biochemistry volume 35, pages 661–668 (2009)Cite this article

Abstract

Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15°C (cold water (CW) group), whereas the second group was subjected to a temperature of 20°C (warm water (WW) group) before sperm sampling. Reactivation were achieved after incubation of firstly activated sperm in media with osmotic pressure adjusted up to 300 mOsm*kg−1 by increasing K+ concentration. Statistically significant reduction of spermatozoa concentration in CW samples versus WW (from 46.0 ± 12.5 (15°C) to 59.3 ± 7 109 (20°C) spermatozoa /ml) have been observed. The sperm of the CW group required a significantly longer incubation time (37 min) under isotonic conditions to achieve a maximum percentage of potent motility at repeated activation than the WW group (23 min). After activation of sperm motility, an increase of respiration rate up to maximum level has been found, this level remained the same under condition of recovering the potential to repeated activation. During the sperm movement respiration rate, in CW group (6.1 nmolO2/min/109spermatozoa) and WW (3.9 nmolO2/min/109spermatozoa), was significant higher compared to nonactivated sperm (2.4 nmolO2/min/109spermatozoa for CW and 1.1 nmolO2 /min/109spermatozoa for WW). And keeping males for 7 days at 15°C increase the respiration rate of sperm.

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Acknowledgements

Thanks are expressed to projects: USB RIFCH MSM 6007665809, IAA608030801 and QH82119 from Czech Republic. The authors deeply thank Prof. O. Linhart, S.M.H. Alavi, and J. Sales for their helpful comments and discussions.

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Authors and Affiliations

  1. University of South Bohemia in Ceske Budejovice Research Institute of Fish Culture and Hydrobiology at Vodnany (USB RIFCH), Zatisi 728/II, 389 25, Vodnany, Czech Republic

    Sergey Boryshpolets & Boris Dzyuba

  2. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 23 Pereyaslavskaya str., Kharkov, 61015, Ukraine

    Boris Dzyuba & Sergey Drokin

Authors
  1. Sergey Boryshpolets
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  2. Boris Dzyuba
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  3. Sergey Drokin
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Correspondence to Sergey Boryshpolets.

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Boryshpolets, S., Dzyuba, B. & Drokin, S. Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps. Fish Physiol Biochem 35, 661–668 (2009). https://doi.org/10.1007/s10695-008-9292-4

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  • DOI: https://doi.org/10.1007/s10695-008-9292-4

Keywords

  • Carp
  • Sperm
  • Physiology
  • Aquaculture

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