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Increased growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor I (IGF-I) gene transcription after hyperosmotic stress in the Brazilian flounder Paralichthys orbignyanus

Fish Physiology and Biochemistry volume 35, pages 501–509 (2009)Cite this article

Abstract

Growth hormone (GH) action is the result of an intracellular cascade initiated just after its interaction with the growth hormone receptor (GHR) located on the surface of target cells. This cascade culminates with the transcription of target genes, such as the insulin-like growth factors (IGFs), which are responsible for most GH biological effects. In addition to its central role in growth, fish GH is also involved with osmoregulatory control. Within this context, the objective of the present work was to isolate GH, GHR, and IGF-I cDNAs from the Brazilian flounder Paralichthys orbignyanus and evaluate whether these genes are induced by hyperosmotic stress. The obtained results indicated that GH mRNA had a significant peak only 24 h after hyperosmotic stress. In gills, GHR mRNA was significantly increased after 7 days. In liver, GHR and IGF-I mRNAs were significantly increased in 72 h and both reached even higher levels after 7 days. These results indicate that hyperosmotic stress can increase GH sensitivity in the gills and liver of P. orbignyanus and, consequently, improve IGF-I production. The management of this parameter could be useful in achieving better growth performance for this and other commercially important species in which GH has a direct correlation with osmoregulatory mechanisms.

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Acknowledgments

This work was supported by Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Proc. No. 01/1602.5—Brazil), Universidade Federal do Rio Grande (FURG, Brazil) and Programa de Pós-Graduação em Aqüicultura (PPGAq-FURG).

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Author notes

  1. Rodrigo Maggioni

    Present address: School of Forensic and Investigative Science, University of Central Lancashire, Preston, PR1 2HE, UK

Authors and Affiliations

  1. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande—FURG, Rio Grande, RS, Brazil

    Karina M. Meier, Márcio A. Figueiredo, Michel T. Kamimura & Luis F. Marins

  2. Faculdade de Biociências, Centro de Biologia Genômica e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Jomar Laurino

  3. Faculdade de Educação Ciências e Letras do Sertão Central, Universidade Estadual do Ceará, Quixada, CE, Brazil

    Rodrigo Maggioni

  4. Centro de Biotecnologia, Universidade Federal de Pelotas, RS, Pelotas, Brazil

    Luciano S. Pinto & Odir A. Dellagostin

  5. Instituto de Oceanografia, Universidade Federal do Rio Grande—FURG, Rio Grande, RS, Brazil

    Marcelo B. Tesser & Luís A. Sampaio

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  1. Karina M. Meier
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Correspondence to Luis F. Marins.

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Meier, K.M., Figueiredo, M.A., Kamimura, M.T. et al. Increased growth hormone (GH), growth hormone receptor (GHR), and insulin-like growth factor I (IGF-I) gene transcription after hyperosmotic stress in the Brazilian flounder Paralichthys orbignyanus . Fish Physiol Biochem 35, 501–509 (2009). https://doi.org/10.1007/s10695-008-9287-1

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Keywords

  • Hyperosmotic stress
  • Growth
  • Osmoregulation
  • Semi-quantitative RT-PCR

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