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Curcumin analogue inhibits lipid peroxidation in a freshwater teleost, Anabas testudineus (Bloch)—an in vitro and in vivo study

Fish Physiology and Biochemistry volume 35, pages 413–420 (2009)Cite this article

Abstract

The effect of a synthetic curcumin analogue (salicylcurcumin) on fish lipid peroxidation was investigated in both in vitro and in vivo conditions using a teleost model Anabas testudineus (Bloch). Curcumin analogue inhibited the formation of lipid peroxidation products and thiobarbituric acid reactive substances (TBARS) content at the three concentrations (10−2 M, 10−3 M and 10−4 M) in vitro. TBARS content was reduced by 80% in the liver and 68% in brain by the higher concentration of salicylcurcumin. For in vivo study, salicylcurcumin (0.5%) was supplemented along with the basal feed for a period of 60 days. It produced a 60% reduction in liver TBARS content. The antioxidant enzyme superoxide dismutase (SOD) was stimulated, whereas catalase (CAT) and glutathione peroxidase (GPx) were inhibited. Glutathione (GSH) was reduced and glutathione reductase (GR) unchanged. Even though there was an increase in SOD activity, the CAT and GPx did not increase accordingly, maybe due to the direct scavenging of H2O2 by salicylcurcumin. The protein content also increased in the curcumin-fed animals, indicating a positive growth-promoting effect. Therefore, it would be beneficial to supplement salicylcurcumin along with the aquaculture feed in order to help the fish to cope with adverse conditions in the environment. This would increase the survival rate, disease resistance and ultimately the growth rate.

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Acknowledgements

The authors thank The Council of Scientific and Industrial Research (CSIR), New Delhi, for providing the Senior Research Fellowship (MM) and University Grants Commission-Special Assistance Programme (UGC-SAP) for the infrastructural facilities.

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Authors and Affiliations

  1. Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, 695581, India

    Maniyan Manju & Oommen Vilaverthottathil Oommen

  2. Department of Chemistry, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, 695581, India

    Thomas G. Sherin & Kallikat N. Rajasekharan

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  1. Maniyan Manju
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  2. Thomas G. Sherin
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  3. Kallikat N. Rajasekharan
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  4. Oommen Vilaverthottathil Oommen
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Correspondence to Oommen Vilaverthottathil Oommen.

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Manju, M., Sherin, T.G., Rajasekharan, K.N. et al. Curcumin analogue inhibits lipid peroxidation in a freshwater teleost, Anabas testudineus (Bloch)—an in vitro and in vivo study. Fish Physiol Biochem 35, 413–420 (2009). https://doi.org/10.1007/s10695-008-9266-6

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Keywords

  • Anabas testudineus
  • Antioxidant
  • Reactive oxygen species
  • Growth

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