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Effect of in vitro exposure to Vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (Sparus aurata L.) intestine

Fish Physiology and Biochemistry volume 35, pages 541–549 (2009)Cite this article

Abstract

The everted gut sac technique has been used to investigate the effect of Vibrio vulnificus on water and electrolyte (Na+, K+, Cl−, HCO3 −) transport on the intestine of sea bream (Sparus aurata L.). Both the anterior and the posterior intestine were incubated in a medium containing 108 V. vulnificus cells ml−1 at 25°C for 2 h. The presence of V. vulnificus resulted in a significant reduction (P < 0.05) of water absorption in the anterior intestine, while sodium absorption in the anterior (P < 0.01) and posterior (P < 0.05) intestine was elevated. Chloride absorption was increased, but the changed was not significant, while potassium absorption decreased significantly (P < 0.05), but only in the posterior intestine. Incubation the sea bream intestine with V. vulnificus did not affect carbonate secretion in the anterior segment, whereas high secretion was stimulated in the posterior segment (P < 0.01). Histological evaluations demonstrated damage in the anterior intestine of sea bream that was characterized by the detachment of degenerative enterocytes, alterations in the microvilli, and the presence of a heterogenous cell population, indicating inflammation. Based on our results, we conclude that V. vulnificus caused cell damage to the intestine of sea bream and that the anterior intestine is more susceptible than the posterior part of the intestine. Several hypotheses are suggested to explain our observations, such as the presence of higher numbers of villosities in the anterior intestine than in the posterior one and/or the presence of endogenous bacteria in the posterior intestine which may have a protector role.

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Acknowledgments

The authors thank Mr. Boukottaya Samir for his careful presentation of the manuscript.

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Authors and Affiliations

  1. Laboratory of Physiology, Faculty of Dental Medicine, Monastir, Tunisia

    Fathia Khemiss

  2. Laboratory of Physiology, Faculty of Pharmacy, Monastir, Tunisia

    Salwa Ahmadi, Raja Massoudi & Dalila Saïdane

  3. Laboratory of Histology–Embryology, Faculty of Dental Medicine, Monastir, Tunisia

    Sonia Ghoul-Mazgar & Sihem Safta

  4. School of Pharmacy Isfahan Medical Sciences, Iran University, Isfahan, Iran

    Ali Asghar Moshtaghie

Authors
  1. Fathia Khemiss
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  2. Salwa Ahmadi
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Correspondence to Fathia Khemiss.

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Khemiss, F., Ahmadi, S., Massoudi, R. et al. Effect of in vitro exposure to Vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (Sparus aurata L.) intestine. Fish Physiol Biochem 35, 541–549 (2009). https://doi.org/10.1007/s10695-008-9265-7

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  • DOI: https://doi.org/10.1007/s10695-008-9265-7

Keywords

  • Electrolyte transport
  • Everted gut sac
  • Sparus aurata (L.)
  • Vibrio vulnificus
  • Water transport

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