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Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with Enterococcus sp.

Fish Physiology and Biochemistry volume 35, pages 219–222 (2009)Cite this article

Abstract

This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 × 103 and 1 × 106 colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 103 and 1 × 106 CFU/ml.

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Acknowledgments

The authors thank “National Council of Scientific and Technologic Development/CNPq-300281/04-8) for the grant to M.L. Martins, Microbiology Laboratory of Marine Shrimp Laboratory, UFSC, SC, for aid in bacteria isolation and Roberto Hoppe (Fundação 25 de julho, Joinville, SC) for fish donation.

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Authors and Affiliations

  1. Laboratory of Diagnosis and Pathology in Aquaculture, Aquaculture Department, Federal University of Santa Catarina (UFSC), Rod. SC 404, Km 3, Florianópolis, SC, 88040-900, Brazil

    Maurício L. Martins

  2. Aquaculture Department, Federal University of Santa Catarina (CCA, UFSC), Rod. SC 404, Km 3, Florianópolis, SC, 88040-900, Brazil

    Felipe N. Vieira, Gabriela T. Jerônimo, Geovana Dotta, Gisele M. Speck & Gilberto Pereira Jr

  3. Marine Shrimp Laboratory, Aquaculture Department, CCA, UFSC, Beco dos Coroas, Barra da Lagoa, Florianópolis, SC, 88062-601, Brazil

    Felipe N. Vieira, José L. P. Mouriño, Adolfo J. M. Bezerra, Fabíola S. Pedrotti & Celso C. Buglione-Neto

Authors
  1. Maurício L. Martins
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  2. Felipe N. Vieira
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  3. Gabriela T. Jerônimo
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  4. José L. P. Mouriño
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  5. Geovana Dotta
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  6. Gisele M. Speck
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  7. Adolfo J. M. Bezerra
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  8. Fabíola S. Pedrotti
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  9. Celso C. Buglione-Neto
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  10. Gilberto Pereira Jr
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Correspondence to Maurício L. Martins.

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Martins, M.L., Vieira, F.N., Jerônimo, G.T. et al. Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with Enterococcus sp.. Fish Physiol Biochem 35, 219–222 (2009). https://doi.org/10.1007/s10695-008-9262-x

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  • DOI: https://doi.org/10.1007/s10695-008-9262-x

Keywords

  • Infection
  • Leukocyte
  • Phagocytosis
  • Tilapia

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