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Morphological and histochemical characteristics of the epithelium of ovarian lamellae of Genypterus blacodes (Schneider, 1801)

Fish Physiology and Biochemistry volume 35, pages 359–367 (2009)Cite this article

Abstract

The physiological significance of the glycoproteins (GPs) secreted by the epithelium of ovarian lamellae is discussed in reference to the reproductive biology of G. blacodes. Histochemical procedures for localising and characterising GPs were used to determine the cytoplasmic components of cells of the epithelium that covers the ovarian lamellae of pink cuskeel, Genypterus blacodes (Schneider, 1801) (Pisces, Ophidiidae), during spawning. This species is one of the most valuable demersal fish resources in the Argentine Sea, mainly due its large size and flesh quality. GPs with oxidizable vicinal diol groups, sialic acid with or without O-acyl substituents, O-acyl sugars, neutral sugars and GPs with carboxyl and sulphate groups were detected. Light microscope examination showed morphological changes in the epithelium of ovarian lamellae during the spawning season, associated with a secretory activity of mucus. Optical density studies revealed the presence of polyploid cells encompassing those morphological changes. Results of the present study suggest that the epithelium of ovarian lamellae of G. blacodes performs a secretory role, which is intensified during ovarian maturity, suggesting that G. blacodes could release masses of eggs enveloped in mucus.

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Acknowledgments

This work was partially supported by a grant from the University of Mar del Plata, Argentina. ELP, CGB and GJM are research members of CONICET.

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Authors and Affiliations

  1. Departamento de Biología. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, 3 piso, 7600, Mar del Plata, Argentina

    R. O. Freijo, A. M. García & A. O. Díaz

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    A. M. García, E. L. Portiansky, C. G. Barbeito & G. J. Macchi

  3. Instituto de Patología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina

    E. L. Portiansky & C. G. Barbeito

  4. Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Mar del Plata, Argentina

    G. J. Macchi

Authors
  1. R. O. Freijo
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  2. A. M. García
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  3. E. L. Portiansky
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  4. C. G. Barbeito
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  5. G. J. Macchi
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  6. A. O. Díaz
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Correspondence to A. O. Díaz.

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Freijo, R.O., García, A.M., Portiansky, E.L. et al. Morphological and histochemical characteristics of the epithelium of ovarian lamellae of Genypterus blacodes (Schneider, 1801). Fish Physiol Biochem 35, 359–367 (2009). https://doi.org/10.1007/s10695-008-9256-8

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  • DOI: https://doi.org/10.1007/s10695-008-9256-8

Keywords

  • Genypterus blacodes
  • Epithelium of ovarian lamellae
  • Glycoproteins
  • Histochemistry
  • Ovary

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