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Polymorphisms within promoter of Japanese flounder (Paralichthys olivaceus) ovary cytochrome P450-c19 (CYP19a) gene associated with reproductive traits

Fish Physiology and Biochemistry volume 35, pages 333–340 (2009)Cite this article

Abstract

Cytochrome P450 aromatase, which is encoded by the CYP19a gene, converts androgens to estradiol. Considerable evidence suggests that estrogens play an important role in fish reproductive process. Therefore CYP19a is an excellent candidate gene for reproductive traits. Variants in the promoter of the CYP19a gene might also be involved in the control of aromatase expression and affect regulatory mechanism linking cholesterol metabolism to the synthesis of sex steroids. In this study, nine single-nucleotide polymorphisms (SNPs) were detected with polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP), namely A-680G, G-672A, AGTAGT-649 inserting or deleting, T-623C, C-410A, T7-454A, T-402C, TTTCCAGACTGA-345 inserting or deleting, and G-297C. Nine SNPs within the promoter of the CYP19a gene were tested for association with four reproductive traits [serum testosterone (T), serum 17β-estradiol (E2), hepatosomatic index (HSI), and gonadosomatic index (GSI)] in a population of 50 female Japanese flounder individuals. A locus, P3 (TTTCCAGACTGA-345 inserting or deleting, G-297C), was significantly associated with 17β-estradiol (E2) level (P < 0.05) in female Japanese flounder. In addition, there was significant association between one diplotype based on nine SNPs and reproductive trait. The genetic effect for E2 level of diplotype D3 was significantly higher than those of other diplotypes (P < 0.05). Results indicate that these genetic effects of those variants on E2 level may help to explain CYP19a gene status in the reproductive endocrinology of Japanese flounder.

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Acknowledgments

This work was supported by the National Key Technologies R & D Program (2006BAD09A01).

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Authors and Affiliations

  1. Key Laboratory of Mariculture Ministry of Education, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Feng He, Hai Shen Wen, Shuang Lin Dong, Bao Shi, Cai Fang Chen, Lian Shun Wang, Jun Yao, Xing Jiang Mu & Yu Guo Zhou

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  1. Feng He
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  2. Hai Shen Wen
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  3. Shuang Lin Dong
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  8. Xing Jiang Mu
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Correspondence to Hai Shen Wen.

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He, F., Wen, H.S., Dong, S.L. et al. Polymorphisms within promoter of Japanese flounder (Paralichthys olivaceus) ovary cytochrome P450-c19 (CYP19a) gene associated with reproductive traits. Fish Physiol Biochem 35, 333–340 (2009). https://doi.org/10.1007/s10695-008-9237-y

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  • DOI: https://doi.org/10.1007/s10695-008-9237-y

Keywords

  • Japanese flounder
  • CYP19a gene promoter
  • SNPs
  • Diplotype
  • Reproductive traits

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