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Effects of light and temperature conditions on the expression of GnRH and GtH genes and levels of plasma steroids in Odontesthes bonariensis females

Fish Physiology and Biochemistry volume 35, pages 101–108 (2009)Cite this article

Abstract

In this study we examined the endocrine mediation between environmental factors (temperature and photoperiod) and the brain–pituitary–gonadal axis in females of pejerrey Odontesthes bonariensis. Changes in the expression of brain gonadotropin-releasing hormones (GnRHs) and gonadotropin (GtH) subunit [follicle stimulating-β (FSH-β), luteinizing hormone-β (LH-β), glycoprotein hormone-α (GPH-α)] genes, plasma gonadal steroids [estradiol (E2) and testosterone (T)], gonadal histology, and gonadosomatic index (GSI) in adult females exposed to combinations of short-day (8 h) or long-day (16 h) photoperiods and low (12°C) or high (20°C) temperatures after winter conditions (8 h light, 12°C) were analyzed. Pejerrey females kept under the short photoperiod had low GSIs, and their ovaries contained only previtellogenic oocytes regardless of the experimental temperature. In contrast, females exposed to the long photoperiod had high GSIs and ovaries with vitellogenic oocytes at both temperatures. These fish also showed a significantly higher expression of sGnRH, pjGnRH, cGnRH-II (the three different GnRH variants found to date in the pejerrey brain), FSH-β, LH-β and GPH-α genes and plasma E2 levels than those at the shorter photoperiod. No significant changes were observed in plasma T levels. Based on these results, we concluded that the increase in day length but not that of temperature triggers the maturation of pejerrey females after the winter period of gonadal rest and that this occurs by an integrated stimulation of the various components of the brain–pituitary–gonad axis.

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Acknowledgments

The authors would like to thank Gabriela López and Takahashi Shinoda for their technical assistance. This work was partially supported by grants given to L.A.M (University of San Martín #SA06/009), C.A.S. (Ministry of Education, Culture, Sports, Science and Technology of Japan #15201003), and G.M.S. (CONICET PIP #5425 and ANPCyT PICTR-528).

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Authors and Affiliations

  1. Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas–Instituto Tecnológico de Chascomús, (B7130IWA) Chascomus, Buenos Aires, Argentina

    L. A. Miranda & G. M. Somoza

  2. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-Ku, Tokyo, Japan

    C. A. Strüssmann

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  1. L. A. Miranda
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  2. C. A. Strüssmann
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  3. G. M. Somoza
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Correspondence to L. A. Miranda.

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Miranda, L.A., Strüssmann, C.A. & Somoza, G.M. Effects of light and temperature conditions on the expression of GnRH and GtH genes and levels of plasma steroids in Odontesthes bonariensis females. Fish Physiol Biochem 35, 101–108 (2009). https://doi.org/10.1007/s10695-008-9232-3

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  • DOI: https://doi.org/10.1007/s10695-008-9232-3

Keywords

  • Environmental cues
  • Gonadotropin-releasing hormone
  • Gonadotropins
  • Pejerrey
  • Reproduction
  • Sex steroids

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