Abstract
In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons benzo[a]pyrene (B[a]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of B[a]P (20 mg kg−1) for 6, 12, 24, and 48 h. B[a]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS) after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel electrophoresis comet assay. B[a]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up to 5.55 ± 0.67 μg g−1 dry weight after only 6 h exposure and 4.67 ± 0.68 μg g−1 dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after 48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to 12.17 %DNA in the tail.
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Acknowledgements
Special thanks are due to the Ministry of higher Education, Scientific Research and Technology of Tunisia Unité de Recherche en Biochimie et Toxicologie Environnementale who allowed this study to be carried out. This research was supported by Cooperation Inter-Universitaire Franco-Tunisienne (CMCU) within the framework of project 04G0907.
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Banni, M., Bouraoui, Z., Ghedira, J. et al. Acute effects of benzo[a]pyrene on liver phase I and II enzymes, and DNA damage on sea bream Sparus aurata . Fish Physiol Biochem 35, 293–299 (2009). https://doi.org/10.1007/s10695-008-9210-9
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DOI: https://doi.org/10.1007/s10695-008-9210-9