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Determination of hormones inducing oocyte maturation in Chalcalburnus tarichi (Pallas, 1811)

Fish Physiology and Biochemistry volume 34, pages 447–454 (2008)Cite this article

Abstract

Chalcalburnus tarichi is an endemic cyprinid species living in the Lake Van basin, in eastern Anatolia, Turkey. The present study was undertaken to determine which hormones induce oocyte maturation in C. tarichi. The levels of 17α,20β,21-trihydroxyprogesterone (20β-S), progesterone (P), 17α-hydroxyprogesterone (17α-HOP), 11-deoxycortisol (11-DOC), and 17α-hydroxy-20β-dihydroprogesterone (17,20β-P) were measured in fish caught from Lake Van and the Karasu River, and injected with human chorionic hormone (hCG) (1,000 and 1,500 IU/kg). Oocytes of fish caught from the lake were also incubated in vitro with different doses (50, 200, and 1,000 ng/ml) of 20β-S, 17α-HOP, 11-DOC, and 17,20β-P. 11-DOC was found to be the most effective hormone among those measured for inducing oocyte maturation in vivo and in vitro. 17,20β-P could not be determined in the plasma of any fish in vivo (P < 0.05). 1,000 IU/kg dose of hCG given by injection caused a statistically significant increase in all plasma hormone levels (P < 0.05). It was found that there was a significant decrease in the P level only at 1,500 IU/kg dose of hCG injected (P < 0.05), while the level of other hormones increased at this dose (P < 0.05). It was also determined that all the hormones were effective in germinal vesicle breakdown (GVBD) in in vitro oocyte culture (P < 0.05). However, 11-DOC was found to be the most effective hormone in GVBD at a dose of 200 ng/ml (70% GVBD). In conclusion, 11-DOC synthesized during final oocyte maturation in C. tarichi was found to be a potent inducer of GVBD, which shows that 11-DOC may be described as an oocyte maturation steroid in this species.

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Acknowledgements

The writers wish to express their heartfelt thanks to the Directorate of Scientific Research Projects of Yüzüncü Yıl University for kindly providing the financial support required for this study to be conducted (2003 FED-035). The authors thank Dr. Peyami BATTAL and research assistant M. Emre EREZ, who conducted the HPLC analysis of plasma steroid levels. Additionally, the manuscript was greatly improved by recommendations from the anonymous referees.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Literature, Yüzüncü Yıl University, 65080, Van, Turkey

    G. Ünal, A. R. Oğuz & B. Kaptaner

  2. Department of Histology and Embryology, Faculty of Medicine, Yüzüncü Yıl University, 65080, Van, Turkey

    E. Erdoğan

  3. Department of Fisheries, Faculty of Agriculture, Yüzüncü Yıl University, 65080, Van, Turkey

    E. Kankaya & M. Elp

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  1. G. Ünal
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  2. E. Erdoğan
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  3. A. R. Oğuz
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  4. B. Kaptaner
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  5. E. Kankaya
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  6. M. Elp
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Correspondence to G. Ünal.

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Ünal, G., Erdoğan, E., Oğuz, A.R. et al. Determination of hormones inducing oocyte maturation in Chalcalburnus tarichi (Pallas, 1811). Fish Physiol Biochem 34, 447–454 (2008). https://doi.org/10.1007/s10695-008-9203-8

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  • DOI: https://doi.org/10.1007/s10695-008-9203-8

Keywords

  • Chalcalburnus tarichi
  • hCG
  • MIS
  • Oocyte maturation

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